Supplementary data for Bennett et al. Carbon adsorption by Fe oxyhydroxide stalks produced by a
lithotrophic iron-oxidizing bacteria
Figure S1 - Raw C 1s NEXAFS spectra of M. ferrooxydans stalks during various stages of the bacteria’s
growth. Data processing was carried out using cluster analysis carried out by IDL aXis2000 software.
Clusters with similar spectral properties were identified (i) and their raw NEXAFS spectra are shown(ii).
a) Early log phase, b) Mid-log phase c) Stationary phase d) 2.5 Week old sample and e) 5 month old
sample
a) Early log phase
i)Cluster 1
Cluster 2
Cluster 3
ii)
1.2
Absorbance
1
0.8
0.6
0.4
Cluster 2
0.2
Cluster 3
0
282
287
292
Energy (eV)
297
b) Mid-log phase
i)Cluster 1
Cluster 2
Cluster 3
ii)
1.4
1.2
Absorbance
1
0.8
0.6
0.4
Cluster 2
0.2
Cluster 3
0
282
287
292
Energy (eV)
297
c) Stationary phase
i)Cluster 1
Cluster 2
Cluster 3
ii)
1.6
1.4
Absorbance
1.2
1
0.8
0.6
0.4
Cluster 2
0.2
Cluster 3
0
282
287
292
Energy (eV)
297
d) 2.5 Week old sample
i)Cluster 1
Cluster 2
Cluster 3
ii)
1.6
1.4
Absorbance
1.2
1
0.8
0.6
0.4
Cluster 2
0.2
Cluster 3
0
282
287
292
Energy (eV)
297
e) 5 month old sample
i)Cluster 1
Cluster 2
Cluster 3
ii)
1.2
Absorbance
1
0.8
0.6
0.4
Cluster 2
0.2
Cluster 3
0
282
287
292
Energy (eV)
297
Figure S2 - Raw C 1s NEXAFS spectra of stationary phase (5 day) M. ferrooxydans stalks prepared at the
beamline. Data processing was carried out using cluster analysis carried out by IDL aXis2000 software.
Clusters with similar spectral properties were identified (i) and their raw NEXAFS spectra are shown(ii).
i)Cluster 1
ii)
Cluster 2
Cluster 3
Figure S3 - Raw C 1s NEXAFS spectra of stationary phase (5 day) M. ferrooxydans stalks prepared at the
beamline and sandwiched between two membrane windows. Data processing was carried out using
cluster analysis carried out by IDL aXis2000 software. Clusters with similar spectral properties were
identified (i) and their raw NEXAFS spectra are shown(ii).
i)Cluster 1
Cluster 2
Cluster 3
ii)
Absorbance
1
Cluster 1
0.8
Cluster 2
0.6
Cluster 3
0.4
0.2
0
-0.2
282
287
292
-0.4
-0.6
Energy (eV)
297
Figure S4 - Raw Ca 1s NEXAFS spectra of stationary phase (5 day) M. ferrooxydans stalks prepared using
the two preparation procedures (Prep 1 and Prep 2 – see methods). Data processing was carried out
using cluster analysis carried out by IDL aXis2000 software.
0.8
0.7
Absorbance
0.6
0.5
0.4
0.3
0.2
Prep 1
0.1
Prep 2
0
346
348
350
Energy (eV)
352
354