CURRICULUM VITAE Ms. AYESHA IHSAN Tel: off:(92-41) 2651475-312 Email: aishaehsan@gmail.com PERSONAL PARTCULARS Father’s Name: Date of Birth: N.I.C. Religion: Nationality: Postal Address: Permanent address: Ihsan Ullah 13-06-1980 33100-0566734-0 Islam Pakistani Industrial Biotechnology Division, P.O. Box # 577, NIBGE, Jhang Road, Faisalabad, Pakistan. 18-C, GTL, Steam Power Colony, Mananwala, Faisalabad ACADEMIC RECORD PhD Quaid-e-Azam Univeristy (Thesis Submitted) M. Phil NIBGE (School of Biotechnology) affiliated with Quaid-i-Azam University, Islamabad, 2003-2005 Biotechnology 1st div. M. Sc. University of Agriculture, Faisalabad, 2002, Chemistry Ist div. B. Sc. University of The Punjab, Lahore, 1999, Botany, Chemistry, Zoology. Ist div. Board of Intermediate & Secondary Education, Faisalabad 1997, Pre-Medical, 2nd div. F. Sc. Secondary School Certificate Board of Intermediate & Secondary Education, Faisalabad, 1995, Science Subjects, Ist div. M.Sc THESIS TITLE Validation of gel permeation chromatography for the clean up of pesticide contaminated fatty food commodities M. Phil THESIS TITLE Development of an enzyme biosensor employing different matrices. PhD Thesis Title: Synthesis of functionalized metal nanoparticles/nanoclusters for application in gene/drug delivery system SKILLS ON TECHNIQUES Field Emission Scanning electron Microscopy Atomic Force Microscopy Transmission Electron Microscopy Photon correlation spectroscopy Particles Size Distribution Analyzer PGSTAT (Potentiostat Galvanostat) HPLC (High Performance Liquid Chroamtography) GPC (Gel Permeation Chromatography) FTIR (Fast Fourier Transform Infrared Spectroscopy) FPLC (Fast Protein Liquid Chromatography) UV-Vis Spectrophotometer Helios Gene Gun Isolation of bacterial cultures Genomic and Plasmid DNA extraction from bacterial cultures PCR (Polymerase Chain Reaction) Agarose Gel Electrophoresis. Phase contrast microscopy. Paper chromatography. Cloning of bacterial gene Double beam spectrophotometer Academic Honours: Merit Scholarship from Punjab University in B Sc Higher Education Commission Scholarship in M Sc Foreign Trainings Six Month Training at University of Liverpool, UK for the synthesis and characterization of Nanoparticles especially for the purpose of gene delivery Six Month Training at University of Massachusetts USA to develop fluorescent clusters for bio-imaging/biolabelling and drug delivery. One week operational training on Field Emission Scanning Electron Microscopy at JEOL Factory, Tokyo, Japan One week operation training on Atomic Force Microscope at Shimadzu factory, Japan Publication: S. Rauf, A. Ihsan, K. Akhtar, M.A. Ghauri, M. Rahman, M.A. Anwar and A.M. Khalid, (2006) Glucose oxidase immobilization on a novel cellulose acetate–polymethylmethacrylate membrane. Journal of Biotechnology, 121, 3, 351-360 Irshad Hussain, Syed Zajif Hussain, Habib-ur-Rehman, Ayesha Ihsan, Asma Rehman, Zafar M. Khalid, Mathias Brust and Andrew I. Cooper. In situ growth of gold nanoparticles on latent fingerprints— forensic applications to inkjet printed nanoparticle patterns. Nanoscale, 2010, 2, 2575-2578 Asma Rehman, Muhammad Irfan Majeed, Ayesha Ihsan, Syed Zajif Hussain, Saif-ur-Rehman, Muhammad Afzal Ghauri, Zafar M. Khalid and Irshad Hussain. Living fungal hyphae - templated porous gold microwires using nanoparticles as building blocks. Journal of Nanoparticle Research (25 September 2011), pp. 1-8. Ayesha Ihsan, Irshad Hussain, Waheed S. Khan, Asma Rehman, M. Afzal Ghauri and Chuanbao Cao, Facile synthesis of metallized nanocontainers for the entrapment and controlled release of hydrophobic compounds. Material Letters. Accepted Waheed S. Khana, Chuanbao Cao, Faheem K. Butt, Zulfiqar Ali, Zahid Usman, Ghulam Nabi, Ayesha Ihsan, Asma Rehman, Irshad Hussain, M. Tanveer, Sajad Hussain. Hydrogen storage and PL properties of novel Cd/CdO shelled hollow microspheres prepared under NH3 gas environment. International Journal of Hydrogen Storage (In Press) Waheed S. Khan, Chuanbao Cao, Faheem K. Butt, Zulfiqar Ali, Ayesha Ihsan, M. Tanveer, Imran Aslam, Ghulam Nabi, Asma Rehman, Tariq Mahmood, Sajad Hussain. Preparation of highly pure CdSe hollow structures: Their PL and hydrogen absorption properties. Material Letters (In Press) Waheed S. Khan, Chuanbao Cao, Imran Aslam, Zulfiqar Ali, Faheem K. Butt, Tariq Mahmood, Ghulam Nabi, Ayesha Ihsan, Zahid Usman, Asma Rehman. Single crystalline multipetal Cd nanoleaves prepared by thermal reduction of CdO. Material Research Bulletin (In Press) Waheed S. Khan, Chuanbao Cao, , Faheem K. Butt, Zulfiqar Ali, Ghulam Nabi, Asma Rehman, Ayesha Ihsan, M. Tanveer, Imran Aslam, Sajad Hussain, Zahid Usman, and Tariq Mehmood. Preparation of Single-Crystalline Zinc Nitride Dandelion-Like Nanostructures: Their Photoluminescence and Hydrogen-Absorption Properties. Science of Advance Materials (American Scientific Publishers). (In Press Research Interests: My area of research is nanomaterial synthesis and their applications in biotechnology and advance material fabrication. Being close to the molecular level, nanomaterials have remarkable potential to be used as a tool of molecular biologists and also have remarkable potential to improve the sensing applications. We have screened a phospholipid from human sweat which was found responsible for in situ development of finger prints. This molecule has ability to selfassemble itself in a hollow lamellar fashion. We have successfully template the architecture of this self-assembly using gold nanoparticles as building blocks, and synthesized porous nanocontainers in the size regime of ~100 nm and successfully demonstrated their potential for the encapsulation and controlled release of hydrophobic compounds. Using this approach we have also templated fungal hyphe using gold nanoparticles for the synthesis of gold micro-wires. We have synthesized hollow and porous nanostrucutures using wet chemical and solid phase synthetic approaches and successfully studied their potential for hydrogen storage. Also I have interest to develop fluorescent gold and silver nanoclusters (less than 1nm) for sensing applications. In this regard, my focus is to use protein protected fluorescent clusters for the advance material fabrication while using modern biotechnological tools. Also I am working to develop heavy metal ions sensor using these fluorescent clusters based on the fluorescence quenching. REFEREES Dr. Irshad Hussain, Chair Chemistry Department, LUMS School of Science and Engineering. DHA, Lahore Cant. Office Ph # 042-35608133 Dr. M.A.Ghauri, Principle Scientific Officer, National Institute For Biotechnology & Genetic Engineering (NIBGE), P. O. Box No.577, Jhang Road, Faisalabad. Pakistan. Office Ph. #. 0092-041-651475/252.