Core Facilities at Indiana University
Mass Spectrometry
Website: http://msf.chem.indiana.edu
The MSF is located in A411 and is staffed from 9:30 to 5:30 daily and facility houses
the following equipment: One Thermo MAT-95 XP magnetic sector mass
spectrometer (EI and CI sources available, staff use only), two Waters LCT Classic
electrospray/time-of-flight LC-MS systems (available for walk-up use), one Bruker
Biflex III MALDI-TOF instrument (available for walk-up use), one Agilent
6890/5973 GC-MS (available for walk-up use), and one soon to be installed Agilent
1200/6310 ESI-quadrupole LC-MS system (available for walk-up use). See Dr.
Jonathan Karty (facility manager, jkarty@indiana.edu) or Angela Hansen (Senior
Mass Spectroscopist, asorg@indiana.edu) for more information or to schedule
training. Rates for IU faculty research groups can be found on the Instrumentation
page of the MSF website.
Services provided:
 Walk-up electrospray ionization (ESI) and matrix-assisted laser
desorption/ionization (MALDI) mass spectrometry for both small molecules
and biological samples
 Training for the IU community in the theory and practice of modern mass
spectrometry
 High resolution, high accuracy (±5 parts per million, ppm) mass
spectrometry of small molecules by electron impact, chemical ionization, and
electrospray ionization
 High mass resolution, high mass accuracy gas chromatographic mass
spectrometry
 Low mass resolution gas chromatographic mass spectrometry with both
electron impact and chemical ionization sources
 Capillary-scale (minimum column i.d. = 0.3 mm) high performance liquid
chromatographic mass spectrometry (HPLC-MS) with simultaneous
detection of up to two UV-VIS wavelengths
 Analysis of air and water sensitive compounds by electron impact, chemical
ionization, fast atom bombardment, electrospray ionization, and matrix
assisted laser desorption/ionization.
Biological Mass Spectrometry
Website: http://www.chem.indiana.edu/bms/
The Laboratory for Biological Mass Spectrometry is distributed between the
Chemistry building (C235/C233) and Simon Hall (Si115) on the campus of Indiana
University Bloomington. The facility houses the following equipment: Waters
Synapt G2 HDMS, Thermo Scientific LTQ Orbitrap XL, Thermo Scientific LTQ Velos
Pro, AB SCIEX 4000 QTRAP, Bruker Daltonics micro TOF, and Bruker Daltronics HCT
Ultra. They specialize in the analysis of more complicated (less routine) projects.
Examples of such research-based projects include:
1. Characterization of the post-translational modification states of
a target protein.
2. Large-scale quantification of cell-wide protein levels using
stable isotopes.
3. The enrichment/characterization/and quantification of posttranslationally modified peptides from complex mixtures.
4. Large-scale metabolite profiling as a function of cellular state.
For assistance in experimental design of mass spectrometry-based projects or to
inquire about establishing a research-based collaboration, contact the Director of
Biological Mass Spectrometry: Jonathan Trinidad (trinidad@indiana.edu)
Nanoscale Characterization Facility
Website: http://nano.indiana.edu/Welcome.html
The Nanoscale Characterization Facility (NCF) is housed on the ground floor in
Simon Hall, our multidisciplinary sciences building, and has 2000 ft2 of laboratory
space and 1300 ft2 of cleanroom space. The NCF provides faculty, staff, postdoctoral
fellows, and graduate and undergraduate students with state-of-the-art
instrumentation for generating and characterizing materials having features with
nanometer dimensions. We expect the NCF to grow over the next three to five years
as a university resource, and the instrumentation within the facility will be available
to all research groups at IU. For questions, contact Lyudmilia Bronstein at 812-8553727 or at lybronst@indiana.edu.
Molecular Structure Center
Website: http://www.iumsc.indiana.edu/
The Indiana University Molecular Structure Center laboratory has a full complement
of single crystal and powder diffraction equipment used to characterize crystalline
materials using the techniques of X-ray crystallography. Researchers in the
laboratory can determine the three-dimensional structure of nearly any material
that can be crystallized.
The results of a crystallographic study is a set of atomic coordinates which locate
the atoms of a molecule in the "unit cell" of the crystal. This information can then be
used to generate images of the molecule and to determine distances and angles in
the molecule. In addition, the data allows one to examine the packing of the
molecules in the crystal - information which can often lead to understanding the
properties of the material.
IUMSC Server allows rapid access to the data that are generated in the IUMSC.
Nearly all of the materials studied have been synthesized or isolated by researchers
from other laboratories, usually within the Indiana University system, but often
from laboratories throughout the world. This server supports these researchers to
access to the data as soon as the study is complete.
For questions, please email iumsc@indiana.edu.
Nuclear Magnetic Resonance Facility
Website: http://nmr.chem.indiana.edu/
The NMR Facility provides 7 Varian/Agilent high resolution NMR spectrometers in
from 200 MHz to 800 MHz to perform all modern solution-state experiments. Stateof-the-art 600 MHz and 800 MHz instruments with HCN cold probes dedicated to
BioNMR are housed in the METACyt Biomolecular NMR Laboratory in Simon Hall.
500 MHz, 300 MHz and 2 400 MHz spectrometers reside in Chemistry for walkup
use. A variety of probes are available for these instruments including HCN, HCX and
HCNP and broadband tunable probes as well as the default (1H, 13C) and (1H, 19F,
13C, 31P) probes. All of these instruments have variable temperature systems. The
500 MHz instrument has a 3 axis gradient driver and probe for microimaging. A 200
MHz instrument is also available primarily for undergraduate instruction.
Workstations for offline use are provided in the Chemistry Facility. In addition the
facility maintains site licenses for Mestrenova offline processing software for PC and
Mac and ACD chemical shift prediction.
The NMR facility also features numerous training resources, a wet lab for sample
preparation, a comprehensive web reservation system, easy remote access to data
and data archiving.
Electron Paramagnetic Resonance Room
Website: http://www.chem.indiana.edu/faculty-research/researchfacilities/eprr.asp
The Electron Paramagnetic Resonance Room provides support to research projects
of the chemistry department as well as other departments in chemistry, physics,
material science, and biology. Electron Paramagnetic Resonance is a spectroscopic
technique for studying molecules that contain unpaired electrons, typically
transition-metal ions and organic radicals, yielding structural and dynamic
information. The EPR room is equipped with a Bruker EMX X-band EPR
spectrometer with liquid-helium low temperature (down to 4K) accessories. Frank
Gao (xgao@indiana.edu) is currently in charge of the spectrometer, with
Professor Kenneth Caulton (Caulton@indiana.edu) as the faculty adviser.
Physical Biochemistry Instrumentation Facility
Website: http://www.indiana.edu/~physbio/
Over the past several years there has been a rapid increase on the IUB campus of
research into the structures, stabilities and interactions of biomolecules. The
Physical Biochemistry Instrumentation Facility was established to facilitate and
encourage these research endeavors and to provide a centralized resource for
training and education in modern physical biochemistry. Located in the new, stateof-the-art Simon Hall, it houses state-of-the-art biophysical instrumentation that is
available for use by faculty and students.
Available Equipment:
o NanoTemper Monotlith NT.115 Blue/Red Microscale Thermophoresis
o Perkin-Elmer LS50B Luminescence Spectrometer
o Beckman Coulter Optima XL-I Analytical Ultracentrifuge
o Microcal VP-ITC Isothermal Titration Calorimeter
o Amersham Biosciences Typhoon 9210 Variable Mode Imager
o Jasco J-715 Circular Dichroism Spectropolarimeter
o Varian Cary 100 Bio UV / Visible Spectrometer
o Malvern Instruments Zetasizer Nano-S Dynamic Light Scatterer
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BIACORE 3000
Packard 1600TR Liquid Scintillation Counter
KinTek SF-300X Stopped Flow Spectrometer
Wyatt Technology DAWN HELEOS II (MALS)/OptiLab rEX Multi-angle Light
Scattering Instrument and Refractive Index Detector
Applied Biosystems StepOne Plus RT-PCR Real-time Polymerase Chain Reaction
Instrument
Beckman XPN Preparative Ultracentrifuge
The Center for Genomics and Bioinformatics
Website: http://cgb.indiana.edu/
The CGB was created in 2000 by the Office of the Vice Provost for Research (OVPR)
and the College of Arts and Sciences at Indiana University. It receives additional
support from the School of Informatics and Computing (SOIC), the School of Public
Health, the School of Public and Environmental Affairs (SPEA), and IU Medical
Sciences. The CGB has also received financial support from Lilly Endowment, Inc.
awards to Indiana University.
The primary missions of the CGB are to:
o Act as a service facility that provides IU faculty access to genome technologies
and bioinformatic support.
o Provide consulting and training that supports the development of genomeenabled research programs and grant proposals.
o Develop new genome technologies and bioinformatics tools that are not easily
purchased as a fee for service elsewhere.
The CGB houses the following set of equipment:
Sonication/Shearing
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Covaris S220 (single sample)
Covaris E220 (up to 96 samples)
Robotics
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Beckman Coulter Biomek FX
PCR and Real-time PCR
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8 Eppendorf Mastercyclers
Stratagene Mx3000P Quantitative PCR Thermal Cycle
Quantification and quality control
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Molecular Devices Spectra Max 190 microplate spectrophotometer
NanoDrop ND-1000 Spectrophotometer
Turner BioSystems TBS-380 Mini Fluorometer
Agilent Bioanalyzer 2100
Agilent 2200 TapeStation Nucleic Acid System
Centrifugation
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Beckman Coulter Allegra 25R tabletop centrifuge
Eppendorf Microcentrifuges Model 5424 and Model 5804 tabletop centrifuge
Eppendorf Vacufuge (can spin/dry microtiter plates)
Flow Cytometry Core Facility
Website: http://facs.bio.indiana.edu/
The IU Bloomington Flow Cytometry Core Facility (FCCF) offers flow cytometry
analysis and cell sorting services. It houses the FACS Aria II, a state-of-the-art
sorter/analyzer capable of analyzing fourteen colors and seventeen parameters, the
COPAS Select, a Drosophila embryo and large particle sorter, the LSRII, a thirteen
color, sixteen parameter analyzer with HTS option, the FACSCalibur, a four-color
analyzer, as well as the Z2 Coulter counter technology used for determining cell
concentration nd size distribution. Services include technitian assisted sorting, as
well as training for analyzers, PCR and Coulter instruments. Help with the
experimental design is also available. The facility maintains a workstation with flow
cytometry data analysis software, and also has a copy of this software for use
outside of the facility. For moreinformation and for any questions, please contact
Christiane Hassel (Manager, chassel@indiana.edu).
Light Microscopy Imaging Center
Website: http://www.indiana.edu/~lmic/
Electron Microscopy Center
Website: http://sites.bio.indiana.edu/~cryo/