Supporting Information, Figure S1. Principal components analyses (PCA) of biweekly samples collected May – August for all Qu’Appelle lakes except Pasqua during 2008 (a) and 2009 (b). Symbols and abbreviations as in Fig. 5. 1.0 DOC SRP TDP EDA (a) TDN DIC DON Urea Lept Diatom All(chla) Siliceous NH4 Green ResTime NO3 pH Cyano Cop Vol:Area All(Bcar) Vol Crypto O2 Temp Zmx Cyano(c) Daph Zmn Area Secchi Axis 2 (19.92%) Cond Long -1.0 -1.0 1.0 Axis 1 (27.3%) 1.0 Temp Cyano Cop Cond All(Bcar) Cyano(c) DON PH DOC EDA WRT All(chla) TDP SRP Area TDN NH4 DIC Vol Diatom Urea Secchi Green Lept Crypto Zmx Vol:Area Siliceous Long Zmn NO3 O2 Axis 2 (16.2%) (b) Daph -1.0 -1.0 1.0 Axis1 (32.1%) Bogard et al. Supporting Information, Fig. S1 Supporting Information, Table T1. Urea concentrations, uptake or decomposition (sum of uptake and breakdown) and release rates from different freshwater locations. Data are presented as means (+ S.D.) or as a range. Data were obtained both from published tables and independent interpretation of display items, depending on source. Data was constrained to daytime samples from freshwater sources obtained during spring or summer months, except for groundwater means which represent an annual range, benthic sediment data which are derived from marine sources, and wetland data collected in October. ‘Status’ refers to the trophic status of ecosystem, and includes O = oligotrophic, M = mesotrophic, E = eutrophic, and H = hypereutrophic sites. Urea Location Groundwater Total Plankton (pelagic) Concentration Uptake or Decomposition Regeneration Water Body Status Sample Period (g N L-1) (g N L-1 h-1) (g N L-1 h-1) 43.1 (+ 28.9 ) Well & Spring E-H Annual 28.8 (+ 2.9) Well O-M Jul. 52.5 4.2 Lake Nakaumi O-H Nov. 11.5 - 33.6 1.2 Lake Balaton M-E May-Jun. 24.2 (+ 16.1) 2.9 (+ 0.3) Lake Balaton Eutrophic Basin E Jul.-Sept. 0.9 (+ 0.4) Lake Balaton Mesotrophic Basin M MayAug. 17 Lakes M-H Jun. Pond H Jun. Lake Biwa M-H Jun. Lake Okeechobee H Jun. 29.9 (+ 13.6) 0.288 - 21.6 173.0 1.1 0.7 - 3.7 Phytoplankton (pelagic) 39.8 0.03 2.7 14.4 - 72.0 49.3 Smith Lake H May-Jun. Zooplankton (pelagic) ~0.7 - 3.7 0.02 Lake Biwa M-E Jun. Bacteria (pelagic) 0.7 - 3.7 0.01 Lake Biwa M-E Jun. Pond H Jun. Estuary H MayAug. 173.0 Sediment* 0.7 0.006 12734.4 7207.2 - 13213.4 Macrophyte biofilm Flowing water Oct. 67.2 - 128.8 Lake - benthic E Summer 95.2 - 162.4 Lake - littoral E Summer 132.5 60.0 - 120.1 Wetland 36.3 - 256.3 7.8 - 219.4 2 rivers O Mar. 2 streams O May 23.2 Oct. 9.1 0.1 Han River M 83.4 0.0 Han River H *. Sedimentary rates are µg N m-2 h-1. Table T1 References Wetland Gu B. & Alexander V (1993) Dissolved nitrogen uptake by a cyanobacterial bloom (Anabaena flos-aquae) in a subarctic lake. Applied Environmental Microbiology 59: 422-430. Gu B., Havens K.E., Schelske C.L. & Rosen B.H. (1997) Uptake of dissolved nitrogen by phytoplankton in a eutrophic subtropical lake. 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