Supplemental Tables and Figures
Table S1. “Candidatus ciliate genera” that were detected through V4 SSU rDNA
amplicons in datasets from the three different depths screened for ciliate phylotype
diversity in Piburgersee in March 2011. Amplicons were assigned to a “candidatus
genus”-status, when the maximum sequence similarity to a reference sequence
database of described ciliate morphotypes did not exceed 95%.
Table S2. Ciliate genera and number of species (in brackets) detected in
Piburgersee on 1st March, 2011 in order of relative frequency (mean abundance in
ind. l-1 over the three sampling depths) and abundance in the respective depth. n=6
(0m, 9m), n=3 (21 m), n.d. – not detected.
Fig. S1. Rank-abundance distribution of amplicon datasets from the three different
depths screened for ciliate phylotype diversity in Piburgersee in March 2011. The
distribution follows a long-tail distribution, typically for large environmental amplicon
data sets.
Fig. S2. Log-regression of relative abundances of amplicon ciliate genera and
morphotype ciliate genera. Abundance distributions are incongruent and do not
correlate, suggesting that amplicon read abundances do not reflect morpho-taxon
(organismic) abundances.
1
Table S1. “Candidatus ciliate genera” that were detected through V4 SSU rDNA
amplicons in datasets from the three different depths screened for ciliate phylotype
diversity in Piburgersee in March 2011. Amplicons were assigned to a “candidatus
genus”-status, when the maximum sequence similarity to a reference sequence
database of described ciliate morphotypes did not exceed 95%.
mean amplicon abundance
candidatus-genus
over depths ± std
Monodinium
3.0 ± 2.6
Dileptus
0.3 ± 0.6
Litonotus
0.3 ± 0.6
Ophryoglena
8.7 ± 9.0
Acineta
1.0 ± 1.0
Coleps
638.7 ± 569.1
Placus
7.0 ± 12.1
2
Table S2. Ciliate genera and number of species (in brackets) detected in
Piburgersee on 1st March, 2011 in order of relative frequency (mean abundance in
ind. l-1 over the three sampling depths) and abundance in the respective depth. n=6
(0m, 9m), n=3 (21 m), n.d. – not detected.
Ciliate genus
Mean
Mean
Mean
Mean
abundance
abundance ±
abundance ±
abundance ±
over depths ±
std in 0 m
std in 9 m
std in 21 m
std
depth
depth
depth
Dexiotricha (2)
1001 ± 1392
2.2 ± 5.3
n.d.
3001 ± 1661
Urotricha (10)
905 ± 408
783 ± 278
1312 ± 178
621 ± 506
Balanion (1)
535 ± 402
696 ± 418
843 ± 165
67 ± 33
Halteria (1)
446 ± 350
25 ± 38
445 ± 48
868 ± 321
Pelagostrombidium (2)
207 ± 329
420 ± 465
132 ± 127
68 ± 34
Askenasia (5)
113 ± 69
192 ± 34
121 ± 33
27 ± 11
Cinetochilum (1)
86 ± 54
128 ±40
114 ± 28
15 ± 20
Rimostrombidium (3)
72 ± 34
66 ± 35
75 ± 40
74 ± 23
Urocentrum (1)
56 ± 95
1.2 ± 2.9
n.d.
168 ± 174
Spirostomum (1)
42 ± 61
n.d.
n.d.
125 ± 83
Pelagothrix (1)
23 ± 31
1.2 ± 2.9
4.3 ± 7.4
65 ± 46
Cyclidium (1)
22 ± 39
n.d.
1.5 ± 3.7
63 ± 77
Cyclotrichium (1)
15 ± 18
1.2 ± 2.9
31 ± 17
13 ± 16
Pelagohalteria (1)
13 ± 17
18 ± 12
15 ± 23
6.0 ± 10
Monodinium (2)
11 ± 20
n.d.
3.8 ± 4.2
29 ± 43
Histiobalantium (1)
7.8 ± 12
n.d.
3.8 ± 6.6
20 ± 21
3
Holophrya (1)
5.1 ± 6.8
n.d.
n.d.
15 ± 6.5
Urozona (1)
4.6 ± 7.3
n.d.
n.d.
14 ± 12
Ophryoglena (1)
4.4 ± 6.0
2.3 ± 5.7
2.7 ± 4.2
8.3 ± 9.1
Pelagodileptus (1)
3.4 ± 5.7
3.5 ± 5.9
6.7 ± 6.1
n.d.
Didinium (1)
2.9 ± 6.7
n.d.
n.d.
8.7 ± 15
Lagynophrya (1)
2.4 ± 3.6
n.d.
1.5 ± 3.7
5.7 ± 4.9
Mesodinium (1)
1.8 ± 3.5
n.d.
2.8 ± 4.4
2.7 ± 4.6
Rhabdoaskenasia (1)
1.7 ± 4.3
2.3 ± 5.7
2.7 ± 4.1
n.d.
Balantidion (1)
1.6 ± 3.9
2.3 ± 5.7
n.d.
2.3 ± 4.0
Bursellopsis (1)
1.3 ± 6.2
n.d.
4.0 ± 10
n.d.
Coleps (1)
1.0 ± 2.3
n.d.
n.d.
3.0 ± 5.2
Litonotus (1)
0.9 ± 2.8
1.3 ± 3.3
1.3 ± 3.3
n.d.
Metopus (1)
0.8 ± 1.8
n.d.
n.d.
2.3 ± 4.0
Uronema (1)
0.8 ± 2.6
1.2 ± 2.9
1.3 ± 3.3
n.d.
4