Totalbilirubin meter
User’s manual
Cence
1. Prepare blank calibrator and sample
Please use appointed capillary only: 75mm/60μL, made by HIRSCHMANN
LABORGERATE(GERMANY), REF: 9100260.
1.1 prepare blank
Hold the red end of capillary, fill capillary 2/3 – 3/4 of the total volume of
capillary with distilled water (about 40-45μL). Press the red end into sealing
putty.Twistingthe capillary slightly while pressing it into the putty to improve
the seal (please refer to the operation manual of sealing putty).
1.2 prepare calibrator
Hold the red end of capillary, fill capillary 2/3 – 3/4 of the total volume of
capillary with calibrator (about 40-45μL). Press the red end into sealing putty.
Twisting the capillary slightly while pressing it into the putty to improve the seal
(please refer to the operation manual of sealing putty).
1.3
prepare sample
Hold the red end of capillary, fill capillary 2/3 – 3/4 of the total volume of capillary with
sample blood (about 40-45μL). Press the red end into sealing putty.Twistingthe
capillary slightly while pressing it into the putty to improve the seal.
Place the capillary in the centrifuge head with their sealed end facing
outward. Please refer to the operation manual of sealing putty.
Please refer to the operation instruction of centrifuge.
2 Operation meter
Connect power supply,switch on power. Meter will display as:
Bilirubin meter
Convergent
After 3s, meter display as:
Auto check
Please wait 5s!
Please remove capillary from the measurement cell. Meter start to test the
status of main wavelength and sub-wavelength.
If the auto check is passed, meter will continue to next step.
If auto check is filled, please check if there are used capillary or waste in
measuring cell.
2.1 If auto check is passed, meter will display as:
Bilirubin meter
<test sample>
Press ← or → to select different functions:
� test sample
� test blank
� calibration
� auto check
Press ENTER to confirm the function selection and go to next menu.
3 Test blank
Please do blanking when:
� After the first installation.
� When change a new batch of capillary.
If test blank is selected, meter display as:
Testblank
insert blank
Inset blank prepared in 1.1 with the sealed end on the up position, meter will
start test automatically.
After 5s, blanking will finished.
Remove capillary,meter will back to previous menu automatically.
4 Calibration
Please do calibration when:
� After the first installation.
� When change a new batch of capillary.
If calibration is selected, meter will display as
<STD conc.>
70.00
Modify the calibrator concentration in this menu.
Select the digit need to be modified by press← and →, press ↑ and ↓ to
increase or decrease it. Press ENTER to confirm, then meter display as:
<result unit>
mg/dL
Press ↑ and ↓ to select umol/L or mg/dL.
After the calibrator concentration and unit modification, press ENTER, meter
will go to next step and display:
calibration
insert calibrator
Insert the calibrator prepared in 1.2 with the sealed end on the up position.
Meter will start to test automatically.
After 5s, calibration will finished, the Abs. of calibrator and new factor will
display. Remove capillary,meter will back to previous menu automatically.
5 Test sample
When test sample is selected, meter display as:
Testsample
Insert sample
Insert sample prepared in 1.3 with the sealed end on the up position. Test will
be started automatically. After 5s, test will be finished the result will be
displayed, print out and send to RS232.
Remove sample from measurement cell, meter will ready for next test.
6 Auto check
If auto check is selected, meter will display as:
Auto check
Printer test
First step is printer test. If the printer is working, a string to indicate printer
working will be print out.
After printer test, press ← to back to the previous menu. Press → to test next item:
Auto check
Filter1=1.600
Filter1 is the signal intensity of main wavelength; the range is 1.5-2.5. If it is
lower than 1.500, you must check the meter status.
Press ← to back to the previous menu. Press → to test next item:
Auto check
Filter2=1.600
Filter2 is the signal intensity of reference wavelength; the range is 1.5-2.5. If it
is lower than 1.500, you must check the meter status. If it is lower than filter1,
you must check the meter status.
Press ← to back to the previous menu. Press → to test next item:
Auto check
Vlog=0.100
Vlog is value of lin-log converter, the range is 0-0.5.
Press ← to back to the previous menu. Press → to back to main menu.
7 Measurement principle
Abs= -log(Vlin/Vref)
Conc=F*(Abs of sample-Abs of blank