Fig. 1 An example of RNA electrophoresis of DNAseI and heat treated RNA samples. Selected RNA samples of rep1 samples set, which were collected from the inoculated plants at the indicated time points post inoculum. Samples with the same designation are independent biological replicates of the genotype and collection time point. 273 ng of RNA from each sample was loaded on the gel. Replicate 1 4 HvMPK8 3.5 Relative gene expression Relative gene expression 4 Replicate 2 3 2.5 2 1.5 1 0.5 HvMPK8 3.5 3 2.5 2 1.5 1 0.5 1 1.5 2.5 3.5 4.5 1 Days post inoculation 1.5 2.5 3.5 4.5 Days post inoculation I_L94 I_L94-Pa3 I_L94-Pa7 I_L94 I_L94-Pa3 I_L94-Pa7 M_L94 M_L94-Pa3 M_L94-Pa7 M_L94 M_L94-Pa3 M_L94-Pa7 Fig. 2 Transcriptional dynamics of barley MAPK8 gene in relation to the infection with Puccinia hordei. I_ – plants inoculated with P. hordei, M_ – mock inoculated plants, L94 – susceptible barley line, L94-Rph3 and L94-Rph7 – isolines of L94 harboring R genes Rph3 and Rph7, respectively. Two independent experiments were performed (Replicate 1 and 2), each with two biological replicates per genotype and time point. An average value of two biological replicates is plotted per each genotype and timepoint. The gene expression is shown as relative to the 1 day L94-Pa7 sample (HvMPK2 and HvMPK17).