Activity 5: Ground layer Assessing the ground layer in your Namoi ‘special place’ 5A Introduction A healthy vegetation community needs a healthy ground layer. Looking at the number of different plants (i.e. the species richness) within an area tells us about the biodiversity of that area. Native ground cover has biodiversity and environmental value. Assessing the amount of the ground that is covered by plants, rocks, leaf litter, bare earth and so on provides an indication of the health of the ground layer. The plants in the ground layer are important for several reasons; they help to hold soil and water (preventing soil erosion and decreasing water run-off), add organic matter, help to increase available nutrients, protect seeds and seedlings, and provide habitat for invertebrates and small animals. Organic litter (e.g. leaves and twigs) and coarse woody debris on the ground are also important; they protect seeds and seedlings of native plants, help to maintain soil condition through microbial activity and nutrient recycling, and provide habitat for native fauna. If students are allocated to tasks in small groups, this activity can be done as part of a broader vegetation survey, combining it with a survey of the canopy and midstorey layers (Activity 4). However, if this activity is undertaken independently of the other vegetation survey activities, it is a good idea to start with a class discussion that stresses how important it is to include all layers for a proper assessment of vegetation and habitat. Aim and outcomes The aim of this activity is to assess the ground layer of your site. By the end of Activity 5 you will: understand the importance of the ground layer have learnt about the various parts of the ground layer. Summary of tasks Timing Before visiting the site (Section 5B) Task • Task 5.1 – Ground layer assessment practice During the visit to the site (Section 5C) • Task 5.2 – Ground layer species richness assessment After the visit (Section 5D) • Task 5.4 – Class discussion • Task 5.3 – Ground cover assessment 1 Estimated time Use the accompanying Activity 5 – Record sheet to record the results of the tasks, as appropriate 5B Tasks to undertake before visiting the site Task 5.1 – Ground layer assessment practice You will need: a 50 × 20 m plot (see the handout Preparing a plot) four small hula hoops or four pre-made 1 × 1 m quadrats that are portable and reusable (these can be made from four pieces of PVC pipe cut to 1 m lengths, joined with rope that is knotted at each pipe end – these are light, and are easy to set up and store away) or smart device with Ground Cover app downloaded http://www.namoi.cma.nsw.gov.au/395590.html the Ground layer species richness datasheet from Activity 5 – Record sheet the Ground cover assessment datasheet from Activity 5 – Record sheet To prepare for the species richness survey at the site (Task 5.2) students will set up a practice survey plot within the school grounds. If students are also doing Activities 3 or 4, they can do this task using the practice plot set up in Tasks 3.1 or 4.1. However, if the class is doing Activity 5 as an independent activity, students should set up a practice plot, as outlined in the handout Preparing a plot. The aim of the practice task is to get to know the features of: grasses – sheathing leaves with long thin leaf blades, inconspicuous flowers and seedlike grains herbs – broad-leaved non-woody plants cryptogams – ferns, mosses and lichens organic litter. 4 sample photos or diagrams figure 1, 2, 3, 4 Students will also become familiar with the features they can use to separate the different grasses and herbs present; for example, leaf shape and size, leaf colour, and whether leaves radiate out from the centre or are upright. 2 This task does not require students to know plant names; however, they do need to assign codes to different plants and other features, especially if different individuals or groups are assessing each quadrat. This should be done before starting to assess individual plots for species richness within the quadrats. Task 5.2 explains how to set up codes for the different species and features of the plots. If the school grounds include areas with different species of ground plants, then students can practise the procedure they will use when assessing ground species richness and cover in the field, for Task 5.2. However, if the grounds are uniform lawn, then various objects (e.g. books, pencil cases and lunch boxes) can be placed into the plot to represent plant forms (plant species) rocks or bare ground. Students can then use the codes they have set up for different grasses (G1, G2, G3, etc); herbs (H1, H2, H3, etc); and ferns, mosses and lichens if present, as shown in Figure 5.2. Alternatively, students can try the free app called Ground Cover on a smart device. This app uses the step point method, and describes this simple method. 5C Tasks to undertake at the site Task 5.2 – Ground layer species richness assessment You will need: two 50 × 20 m plots (see the handout Preparing a plot) four small hula hoops or four pre-made 1 × 1 m quadrats that are portable and reusable (these can be made from four pieces of PVC pipe cut to 1 m lengths, joined with rope that is knotted at each pipe end – these are light, and are easy to set up and store away) or smart device with Ground Cover app downloaded http://www.namoi.cma.nsw.gov.au/395590.html Ground layer species richness datasheet from Activity 5 – Record sheet clipboards, extra paper, pencils and pens. Ground layer species richness refers to the number of different grasses, herbs and cryptogams (e.g. mosses and lichens) within the ground layer. Students will use trunks, bark, leaf shape and colour to identify different types of trees within the small plots made using a hula hoop or quadrat (or using the Ground Cover app), and will record their findings. This activity uses the transect lines of the 50 × 20 m plots. Assign student into groups and set up five 1 × 1 m quadrats (using collapsible quadrats or hula hoops) on alternating sides of the transect line of the plot, at the 10, 20, 30, 40 and 50 m marks, as shown in Figure 5.1. 3 50 m 20 40 1 501 1 1 1 1 30 1 10 1 1 1 20 m 1 1 Figure 5.1. Method for ground cover assessment using 1 × 1 m quadrats (in red) for a single transect (e.g. Transect 1). Quadrats are positioned either side of the transect tape at approximately 10 m intervals. The positioning of the quadrats may need adjusting to avoid shrubs (>1 m) in the shrubby transects. Fill in the Ground layer species richness datasheet from Activity 5 – Record sheet. Provide date, site location, group name, transect number assigned (1 or 2), and so on. The aim is to assess the ground layer species richness. Do not include woody shrubs of more than 1 m in height. Students can give each different plant (species) a code; for example, G1 (grass 1), H3 (herb 3), M2 (moss 2), L2 (lichen 2), making notes of plant features for each code as a reminder. Suggest students use codes for other features; for example, BE (bare earth), R (rock) and OL (organic litter). These will be assessed under the ‘ground cover’ section. Ask the students to go back to the individual 1 × 1 m quadrats of the transect and, on the sheet provided, record the number of different plants within each 1 × 1 m quadrat. 4 Task 5.3 – Ground cover assessment You will need: two 50 × 20 m plots (see the handout Preparing a plot) four small hula hoops or four pre-made 1 × 1 m quadrats that are portable and reusable (these can be made from four pieces of PVC pipe cut to 1 m lengths, joined with rope that is knotted at each pipe end – these are light, and are easy to set up and store away) or smart device with Ground Cover app downloaded http://www.namoi.cma.nsw.gov.au/395590.html Ground cover assessment datasheet from Activity 5 – Record sheet clipboard, extra paper, pencils or pens, and ruler. Using the diagram provided on the ground cover (%) worksheet, students will roughly estimate the ground cover percentage by drawing the area each plant form covers (i.e. grasses, herbs, mosses, lichens) and labelling with the agreed code, as shown in the example in Figure 5.2. Remember to include organic litter, rock and bare earth when calculating cover in the 1 × 1 m quadrats. For each quadrat, estimate the percentage of the 1 × 1 m area that is covered by grasses, herbs, mosses, lichens, organic litter, rock and bare ground (bare earth). If there is a lot of variability within the quadrat, simplify the calculation of percentage by dividing the 1 × 1 m quadrat into quarters or sixteenths (as shown in Figure 5.2). Record percentages in the datasheet provided; estimate the percentage of the area covered by each growth form (e.g. grasses, herbs, cryptogams) within each quadrat and record in the datasheet. If present, record the percentage of the area covered by organic litter, and measure the depth of the litter using a ruler. If litter is abundant over the quadrat, then repeat the measurement depth five times and calculate the mean (average) value. Advanced step Cover percentage could also be calculated for each of the different plant ‘species’ identified for species richness. Ensure that each group is recording the same data – cover of growth form (grasses, herbs, cryptogams – or separately as ferns, mosses, lichens) or ‘species’ (e.g. grass 1) so that replicate data can be combined. On the datasheet, the five quadrats provide five replicates; therefore, data from each quadrat will be summed and the mean values calculated. 5 Using data from all quadrats calculate total sum of grasses, total sum of herbs, total sum of rock, total sum of bare earth and total sum of organic litter. Calculate the total cover (%); that is, the percentage of the area of each quadrat covered by plants. Repeat the calculations for Transect 2, if possible, keeping teams the same. The number of different native plants versus the number of exotic plants (weeds) can also be recorded if known – check the plant guide. An extension exercise could be to show the cover percentage of each different species of plant within the plots, with all groups using the codes assigned during the species richness exercise. The results from the different sampling strategies could form part of the overall discussion, asking “Were there differences?”, “If so, why?” There is no right or wrong answer. Use the questions to extend thinking about what was observed, and whether the sampling techniques used are detecting differences. G2 H1 H1 H2 BE M1 H1 R G1 Figure 5.2. Example of ground cover assessment in a 1 × 1 m quadrat. There are five different plants (species) in this plot: herb 1 (H1), herb 2 (H2), grass 1 (G1), grass 2 (G2) and moss 1 (M1) with bare earth (BE) and rock (R) present. If there is no bare earth, but the spaces between the plants are filled with organic litter, then this would be recorded as OL, organic litter. For this plot, the results are: species richness (equals all minus bare earth minus rock) = 5; cover % estimates, R = 4%, H1 = 15%, H2 = 5%, G1= 6%, M1=15%, BE = 55%. 6 5D Tasks to undertake after the visit Task 5.4 – Class discussion Question 1: How many different plants formed the ground layer in Transect 1?................................ Question 2: How many different plants formed the ground layer in Transect 2?................................ Question 3: What was the dominant plant form (grass, herb, moss, etc) in Transect 1? That is, which had the most ‘species’ counts?.......................................................... Question 4: What was the dominant plant form (grass, herb, moss, etc) in Transect 2? That is, which had the most ‘species’ counts?...................................................................... Question 5: Provide two examples of why the ground layer is important ……………………………………………………………………………………………… ……………………………………………………………………………………………… …………………………………………………………………………………………….. …………………………………………………………………………………………….. [Refer to the ground layer introduction.] Question 6: Why is leaf litter an important part of the ground layer? Provide two examples ............................................................................................................................................. …………………………………………………………………………………………….. …………………………………………………………………………………………….. …………………………………………………………………………………………….. [Nutrient recycling – litter breaking down puts nutrients back into the soil; Fauna habitat – many microorganisms and invertebrates live and break down the leaf litter; Retain moisture – moisture is trapped by the mulching effect of the litter.] 7 Question 7: What sort of changes do you think might occur in the ground layer if there was a fire through the plots?................................................................................ [Depends on the intensity of the fire. If it is a ‘cool’ fire, the above-ground plants and litter are removed by the fire, but the heat does not penetrate below the surface, so roots, seeds and microorganisms are protected by the soil. Perennial plants (that grow over multiple seasons) will re-sprout, and seeds will germinate after rain. The first plants to grow will be those that are opportunistic (i.e. able to take advantage of newly available habitat), and grow or spread fast. Seeds of some plants need fire to germinate and respond quickly after a fire. You might expect that annuals (plants that grow, fruit and die in one year) would dominate initially after fire, but perennial plants gradually increase. If weedy species do not have the opportunity to invade, you could expect the area to return to a similar native species composition as seen before the fire. If the fire is ‘hot’, the effects would be different, because the heat would penetrate the soil, and regeneration would be slower and less effective.] 8