Mock Exam 2 BY 123 – Dr. Biga Supplemental Instruction 1. Which of the following are in accordance with the laws of thermodynamics? A) Energy can’t be create be destroyed. B) Things in nature move toward increasing Entropy. C) Entropy of a system may increase as long as the total entropy of the universe increases. D) Both A and B E) All of the above 1. Conservation of Energy - Energy cannot be created or destroyeD) 2. Free energy is declining. Things in nature go towards disorder or randomness or an increase in Entropy. If the entropy of a system increases then the total entropy of the universe must decrease 2. Spontaneous reactions require _________ and __________. A) energy input/ the stability of the system increases B) no energy input/ the stability of the system decreases. C) no energy input/ the stability of the system increases. D) energy input/ the stability of the system decreases. Exergonic – Spontaneous or favored reaction, delta G less that 0… energy released/ no energy added stability increases Endergonic – Nonspontaneous, delta G greater than 0…. had to add energy/ stability decreases 3. Changes in free energy are directly proportional to _________ and inversely proportional to ________. A) Entropy/ Enthalpy B) Disorder/ Heat C) Enthalpy/ Entropy D) None of the above As H increases so does delta G…. as S increases delta G decreases 4. If an enzyme in solution is saturated with substrate, the most effective way to obtain a faster yield of products is to _________. A) heat the solution to 90 C B) add more substrate C) add a non competitive inhibitor D) add an allosteric inhibitor 3. add more of the enzyme The only way to turn more substrates into products is by adding more enzymes so the substrates can bind to the active sites and be converted into products 5. Catabolism is to anabolism as __________ is to _____________. A) free energy/ entropy B) entropy / enthalpy C) endergonic/ exergonic D) exergonic/ endergonic 6. Enzymes are known as biological catalyst and _____________. A) Increase delta G B) Decrease delta G. C) Increase activation energy D) Decrease activation energy. Catalysts only have kinetic properties… they don’t mess with themodynamics 7. Enzymes can turn __________. A) Monosaccharaides into disaccharides B) Disaccharides into monosaccharaides. C) ADP into ATP. D) ATP into ADP. E) A and B only f. All of the above 8. Enzymes can be affected by ___________. A) Salt concentration B) pH C) Increase in temperature D) Decrease in temperature E) Only A B and C f. All of the above Temperature – denaturing of protein pH – pepsin, trypsin Salt Concentration – ions can alter the activity of enzymes 9. A molecule is added to a reaction containing enzymes and substrates. The molecule binds away from the active site and changes the shape of the enzyme’s active site. This molecule is an example of a _____________. A) Competitive inhibitor. B) Non-competitive inhibitor. C) Additional enzyme D) A cofactor Noncompetitive inhibitors do not bind to the active sit but to somewhere else that ultimately changes the shape of the active site which in turn does not allow the substrate to fit in the active site 10. How many ATP’s are made from substrate level phosphorylation in glycolysis? A) 2 B) 4 C) 1 D) 3 Total vs Net Remember. Total 4 are made. You have to put 2 in. So net 2 11. The process of glycolysis takes place in the _______ of the cell and results in the creation of 2 ________ carbon molecules of ___________. A) Mitochondria, 3 ,G3Ps B) Cytosol, 2, Pyruvates C) Mitochondria, 3, Pyruvates D) Cytosol, 3, Pyruvates 12. The movement of Pyruvate into the Mitochondria is a(n) _________ form of transport? A) Passive B) Active C) Cytosolic D) Cooperative Pyruvate is a charged molecule so in eukaryotic cells it must enter the mitochondrion via active transport by way of a transport protein 13. How many ATPs are generated through oxidative phosphorylation by the Oxidation of pyruvate? A) 7 ATP B) 4 ATP C) 5 ATP D) 8 ATP 2 Pyruvate molecules produce 2 NADH x 2.5 ATP per NADH = 5 ATP Because the question asked about oxidative phosphorylation we have to think about the NADH, which 2 are produced? No ATPs are produced by substrate level phosphorylation during this step. 14. How many ATP’s are generated by oxidative phosphorylation only if 7 Acetly COA’s were added to the mitochondria of a cell? A) 52.5 B) 63 C) 57.5 D) 70 Each Acetyl Co A = 3 NADH and 1 FADH. 3 NADH per turn x 2.5 ATP per NADH x 7 turns (ACoas) = 52.5 ATP….. 1 FADH2 per turn x 1.5 ATP per x 7= 10.5 …… 10.5 + 52.5 = 63 ATP. The number 2.5 for NADH and 1.5 for FADH can be found on page 176 in your book. I wanted to be sure that is the numbers in the book. It is. UNLESS Dr. Biga has specifically different number in her lecture, these are what you should use. Also make note that one ATP= 7.3kcal 15. The complete oxidation of 8 glucose molecules would take _____ turns of the Krebb’s Cycle? A) 8 B) 24 C) 12 D) 16 8 x 2…. 2 turns to oxidize a glucose molecule 16. In humans the process of fermentation results in the creation of __________ from ___________. A) NADH / lactate B) ethanol / pyruvate C) lactate /pyruvate D) ATP / lactate Bateria make ethanol 17. A soft drink provides 5 molecules of Citric acid, how many ATP’s are made from substrate level phosphorylation? A) 8 B) 10 C) 5 D) 7 1 ATP is made from each turn of the Krebs’s cycle. Substrate so we are not talking about the ATP that will be generated from NADHs and FADHs produced, that’s oxidative. 18. The color _____________ contains the _____________ energy and the ___________ wavelength. A) Indigo/ highest/ shortest B) Red/ lowest/ longest C) Violet/ highest/ longest D) Green/ lowest/ highest ROYGBIV Violet = Highest energy… and shortest WL 19. Electrons found in photosystem 1 are replenished by _____________, those found in photosystem 2 are replenished by _____________. A) the electron transport chain #1/ the splitting of water B) the splitting of water/ the electron transport chain #1 C) the splitting of Water/ the electron transport chain #2 D) the electron transport chain #2/ the splitting of water 20. In cyclic photophosphorylation, ________ is not created. A) NADH B) 02 C) ATP D) Both A and B Shunts electrons away from the 2nd electron transport chain into the first electron transport chain which results in ATP… remember we use 9 ATP per3 CO2 in the calvin cycle compared to only 6 NADPH this is why we do the cyclic sometimes 21. _____________ is used in ETC/cellular respiration to create ATP by the high H+ concentration in the __________ and the low H+ concentration in _____________ in animal cells. ____________ Is used in photosynthesis to create ATP by the high H+ concentration in the __________ and the low H+ concentration in _____________ in plant cells. A) ATP synthase/ matrix/ intermembrane space/ ATP synthase/ Stroma/ Thylakoid memebrane B) Proton Pump/ intermembrane space/ matrix/ ATP synthase/ intermemebrane space/ stroma C) Proton pump / cytosol/ matrix/ Proton Pump/ outer membrane space/ thylakoid memebrane D) ATP synthase/ intermemebrane space/ matrix/ ATP Synthase/ thylaloid space/ stroma 22. In order to make 5 6-carbon molecules you would use _________ in the Calvin Cycle? A) 30 CO2/ 90 ATP/ 60 NADH B) 20 CO2/ 75 ATP/ 50 NADH C) 30 CO2/ 90 ATP/ 60 NADPH D) 32 CO2/ 90 ATP/ 55 NADPH 6 C02 per Glucose *5 = 30 9 ATP per 3 C02 *10 turns = 90 ATP, 6 *10 = 60 NADPH 23. RUBISCO has the highest affinity for binding _________ to ____________. A) CO2/ Ribulose Phosphate B) 02/ Ribulose Phosphate C) C02/ Ribulose Bisphosphate D) 02/ Ribulose Bisphosphate Sorry guys this was incorrect it is C! Thanks to the student who brought that to my attention 24. The elongation of the cell in mitosis is caused by the ______ of the _____________. The Separation of sister chromatids is caused by the _______ of __________. A) Shortening/ Kinetochore/ Lengthening/ Non kinetochore B) Lengthening/ Non Kinetochore/ Shortening/ Kinetochore C) Shortening/ Non Kinetochore/ Shortening/ Non kinetochore D) Lengthening/ Kinetochore/ Lengthening/ Kinetochore 25. The arrangement of a kinetochore would be ____________. A) increasing B)9+0 C) Semi circular D) 9+2 Kinetochores are made of microtubules… microtubules display a 9+2 arrangement 26. The drug Cytochalasin B blocks the function of actin. Which of the following aspects of the cell cycle would be most disrupted? A) DNA Synthesis B) Spindle formation C) Spindle attatchment D) Cleavage furrow formation E) Cell elongation during anaphase 27. An animal has 2n = 64. How many chromosomes are lined up at the metaphase plate in mitosis? How many sister chromatids are present? A) 32/ 64 B) 16/ 32 C) 64/ 128 D) 72/ 144 28. Meiosis I is the ____________ and Meiosis 2 is the ________________. A) separation of chromatids/ separation of homologous chromosomes B) Separation of homologous chromosomes/ separation of chromatids C) crossing over of sister chromatids/ separation of chromosomes D) separation of chromosomes/ crossing over of sister chromatids 29. A cell that is 2n = 6 results in __________ cells with __________ chromosomes at the end of meiosis II? A) 2/4 B) 4/3 C) 4/4 D) 2/3 30. With each round of spermatogenesis ___________ viable sperm cells are made? A) 1 B) 2 C) 3 D) 4 31. With each round of oogenesis ___________ viable egg cells are made? A) 1 B) 2 C) 3 D) 4 32. Primary spermatocytes have ________ chromosomes and ____________ chromatids, while secondary spermatocytes have ___________ chromatids and ____________ chromosomes. A) 23/46/92/46 B) 23/96/92/23 C) 46/92/46/23 D) 23/92/23/46 Be sure to read careful, notice chromosomes and chromatids are switched around. The chromatids are always double the chromosome. 33. Food is taken in as ____________ energy and is transformed into ___________ energy when an organism uses this energy to move. A. thermal, potential B. potential, mechanical C. chemical, kinetic D. mechanical, thermal E. potential, thermal 34. Which of the following is a reason why high fevers can be dangerous? A. They directly slow down reactions. ( This has to be very high, if it were extreme, you could denature and ultimately slow reactions however the initial rise in temperature will speed up reactions typically) B. They denature enzymes. C. They denature proteins. D. B and C E. All of the above. 35. The concentration of an enzyme is .03 M before a reaction. What would the concentration be after a reaction? A. .06 M B. .003 M C. .03 M D. .3 M E. The exact concentration cannot be calculated, but it would be substantially higher. 36. When O2 binds to hemoglobin, it primes the hemoglobin, making it easier for the rest of the O2 molecules to bind. This is an example of: A. Allosteric binding B. Cooperativity C. Non-competitive inhibition D. Competitive inhibition E. A and C 37. Which type of enzymes play an important role in substrate-level phosphorylation? A. Kinases B. Isomerases C. Mutases D. Enolases E. ATP Synthase 38. Which of the following is not true? A. Reducing agents are electron donors. B. Oxidizing agents are electron acceptors. C. Oxidation is the loss of electrons. D. Reduction is the gain of electrons. E. NAD+ is a reducing agent. 39. A mutation prevents the formation of cristae in the mitochondria. What process would be affected by this mutation? A. Glycolysis B. Photosynthesis C. The electron transport chain D. All of the above. E. None of the above. 40. What is the difference between substrate level phosphorylation and oxidative phosphorylation? A. Substrate level phosphorylation involves the transfer of a phosphate group directly from an organic molecule to ADP using an enzyme and oxidative phosphorylation uses chemiosmosis and ATP synthase B. Oxidative phosphorylation takes place in cellular respiration and substrate level phosphorylation does not. C. Oxidative phosphorylation occurs directly in glycolysis and the citric acid cycle and substrate level phosphorylation does not. D. Substrate level phosphorylation occurs only in cellular respiration while oxidative phosphorylation occurs only in photosynthesis. E. None of the above. 41. Which of the following is most directly responsible for ATP formation in oxidative phosphorylation? A. The series of redox reactions that occurs when the electrons descend down the chain. B. The formation of water. C. Protons moving through ATP synthase. D. All of the above. E. None of the above. 42. True or false: The same number of ATP is always formed in cellular respiration. False 43. What is the difference between lactic acid fermentation and alcoholic fermentation? A. Lactic acid is the starting material in lactic acid fermentation, while ethanol is the starting material in alcoholic fermentation. B. Ethanol fermentation occurs in the presence of oxygen, while lactic acid does not. C. Humans can perform alcoholic fermentation, but not lactic acid fermentation. D. Lactic acid fermentation uses pyruvate as the final electron acceptor, while alcoholic fermentation uses acetaldehyde as the final electron acceptor. E. Facultative anaerobes perform lactic acid fermentation, while obligate anaerobes perform alcoholic fermentation. 44. Cellular respiration is a/an ____________ reaction. Photosynthesis is a/an ___________ reaction. Both cellular respiration and photosynthesis are ____________ reactions. A. Exergonic, exergonic, exergonic B. Exergonic, endergonic, redox C. Endergonic, exergonic, redox D. Hydrolysis, dehydration, redox E. None of the above. 45. A mutant lung cell begins mitosis with 16 chromosomes. How many sister chromatids will be present during metaphase? A. 16 B. 32 C. 72 D. 48 E. There is not enough information to determine the number present during metaphase. 47. What phase of mitosis is this cell in? A. Prophase B. Prometaphase C. Metaphase D. Anaphase E. Telephase 48. Why are C4 plants more suited for hot, dry climates than C3 plants? A. They do not close their stomata in hot, dry weather B. They evolved in cold weather but migrated to the tropics, where they are now more suitable C. They suspend photosynthesis in the heat D. They keep fixing carbon dioxide even when the concentration of carbon dioxide in the leaf is low E. A and D 49. While cellular respiration uses ___________ to drive the synthesis of ATP, photosynthesis utilizes ____________. A. Hydrogen ion gradient, the splitting of water B. Hydrogen ion gradient, hydrogen ion gradient C. Splitting of water, splitting of water D. NADH, FADH2 E. Glucose, light 50. Which of the following are required for the Calvin cycle? A. Light B. CO2 C. ATP D. A and B E. B and C http://www.uic.edu/classes/bios/bios100/lectures/respiration.htm This is a good summary of many processes. ^^^ SLP= substrate level phosphorylation Ox= oxidative Energy Breakdown SLP Ox. Phos Glycolysis 2 ATP Net 2 NADH Ox of Pyruvate 0 ATP 2 NADH Citric Cycle 2 ATP total 6 NADH & 2 FADH2 4 ATP from SLP 10 NADH x 2.5 ATP eq. = 25 ATP 2 FADH2 x 1.5 ATP eq. = 3 ATP 1 NADH = 2.5 ATP eq. 1 FADH2 = 1.5 ATP eq. 4 ATP from SLP + 28 ATP from Ox. Phos = 32 ATP GOOD LUCK!!!!!!!!