Microbes Microbes are microscopic organisms that exist as single cells or cell clusters. Microbiology is the study of microbes, including their lives, features, and impact on the natural world. The characteristics of microbes are numerous and diverse. The large amount of variation within this group helps explain how microbial life has adapted to environmental conditions on Earth. In fact, it is thought that microbes were the earliest life form present. Microbiologists use classification systems to group microbes based on their similar characteristics. The five main groups for microorganisms are Viruses Bacteria Arcahea Fungi Protists Microbiologists often group these organisms into one of two general categories, based on cellular structure. Prokaryotes are organisms that do not have membrane-enclosed structures, called organelles, in their cells. Specifically prokaryotes lack a true nucleus that contains their genetic material. This group includes bacteria and Archaea. Prokaryote Cell Fungi and protists are eukaryotic cells. Eukaryotes contain membrane-enclosed organelles, like the nucleus, and are generally larger and more complex than prokaryotic cells. Viruses are not considered to be living cells, so they are not classified as either prokaryotes or eukaryotes. Eukaryote Cell Recent advances in technology have allowed scientists to separate microbes into five main groups based on genetic information. Scientists use laboratory methods to compare the DNA sequences of microorganisms. Since an organism’s genetic material determines its characteristics, microbes with similar DNA have similar qualities. Without the ability to compare DNA sequences, scientists might not have realized that the bacteria and Archaea DNA classified separately. should be Read the article and answer the following questions. The questions are in order of the article. 1. _____________________ are microscopic organisms that exist as single cells or cell clusters. 2. What is microbiology? 3. What were the earliest life forms present on Earth? In which Geologic Era did they first exist? Precambrian, Paleozoic, Mesozoic, or Cenozoic 4. What do microbiologists use classification systems to group microbes? 5. List the 5 main groups of microorganisms. 6. Microbiologists often group these organisms into one of two general categories – one group has organelles and one group does not. What are the 2 groups? 7. What are membraneenclosed structures found in eukaryotes? 8. In which category do Fungi and protists belong? 9. Which type of organism is most complex, a eukaryotes or a prokaryote? 10. Are viruses prokaryotes or eukaryotes? Why? 11. What do scientists use to separate microbes into the 5 main groups? 12. Microbes with similar __________________ have similar qualities. Viruses Viruses are infectious agents that affect cells of almost all types of organisms. They are the smallest and simplest of microbes. All viruses contain DNA or RNA genetic material. A protein shell called capsid encloses the genetic material. Many viruses also have an outer membrane surrounding them. Most viruses are shaped like rods or spheres. Viruses have characteristics of both living and nonliving things. Even though they contain DNA or RNA, an important characteristic of all loving organisms, viruses cannot reproduce on their own. In order to survive and reproduce, viruses must invade the cells of a living organism, called a host. Viruses cannot take in or metabolize nutrients, produce or get rid of wastes, or move around independently. For these reasons viruses are not considered to be living organisms. In fact, viruses are little more than traveling genetic material, floating harmlessly through the environment until they reach a new host. The typical virus goes through an infectious cycle with several stages: First, a virus must come into contact with a cell. Then, the virus either enters the cell or it attaches to the cell membrane and releases it genetic material into the cell. Once inside the cell, the virus takes over the cellular machinery and forces it to make viral proteins. The viral proteins are assembled to make new copies of the virus. This process of entering a host and replicating is called infection. Eventually, the newly created viruses are released from the cell, sometimes destroying the cell in the process. These new viruses than float around until they come in contact with new potential host cells. The length of time needed for this process to occur ranges from a couple of hours to a few days. Viruses infect all forms of life, from bacteria to people. Viral infections are extremely common in cells throughout the human body. Although viruses often damage or destroy the host cells they infect, this process does not always cause disease. Many viruses enter the body, reproduce, and leave the body without the host ever reacting to them. Viral infection of a host cell is not necessarily bad. In rare cases, these viruses may bring new genetic information that may be of use to the host cell. Read the article and answer the following questions. The questions are in order of the article. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. What are viruses? What are the smallest and simplest microbes? A virus contains ______ or ______ genetic material. What is the name of the protein shell that covers the genetic material? What are the 2 shapes of a virus? Viruses have characteristics of both __________ and ____________ things. What can viruses not do on their own? What is a host? Viruses cannot take in or metabolize ________, produce or get rid of _______, or move around _____. Are viruses considered to be living organisms? What is the first step of an infectious cycle? The virus either enters the cell or it attaches to the ___________ ____________ What does the virus “release into the cell?” What does the virus “force” the cell to do? What is infection? What happens to the newly produced viruses once they are released from the cell? What is the length of time for the infectious cycle? Do viruses always cause disease or cause harm? Bacteria Bacteria are simple, single celled microorganisms. Since their genetic material is not enclosed by a nucleus, bacteria are considered to be prokaryotes. Bacteria can be found alone, in pairs or in groups of three or more. These larger groups can look like clumps or long chains. They can form colonies of different sizes, shapes, and colors, including yellow, green, orange, brown, pink, or white. Bacteria come in three main shapes: rods, spirals, and sphere. The shape of the bacterium comes from the tough cell wall that surrounds the cell, provides protection, and keeps the cell from drying out. Microbiologists sometimes classify these organisms based on their shape. Bacteria are also classified based upon whether they absorb a dye called Gram’s stain. The cell wall of some bacteria prevents from taking in the dye. These are said to be Gram-negative. Bacteria that absorb the stain are called Grampositive. In addition to the cell wall, many bacteria also have flagella (singular: flagellum), or hair-like whips, that are attached to the outside of the cell. By beating its flagellum, a bacterium can propel itself forward. Other bacteria produce slime and glide around their environment. Bacteria are able to reproduce very quickly, sometimes making copies of themselves in less than 15 minutes. The main way they reproduce is asexually by binary fission. In this process, one bacterium duplicates its genetic information, grows larger, and than splits in half to form two daughter bacteria. In this way, bacterial populations can expand to large numbers in a short amount of time when conditions are good. Read the article and answer the following questions. The questions are in order of the article. 1. What are bacteria? 2. Are bacteria prokaryotes or eukaryotes? How do scientists know this? 3. Name 3 ways bacteria can be found? 4. Name 5 colors bacteria are found. 5. What are the 3 shapes of the bacteria? 6. The shape of the bacterium comes from the tough ___________ __________ that surrounds the cell, provides _______________, and keeps the cell from _____________ _________. 7. What is a Gram Stain? 8. What are the difference between a Gram-positive and a Gram-negative bacteria? 9. What is a flagella and what do they help the bacteria do? 10. Other bacteria produce ___________ and _________ around their environment. 11. How fast can some bacteria reproduce? 12. What is the main way they reproduce? 13. What happens in the binary fission process? 14. Bacterial populations can expand to large numbers in a _____________ amount of _________ when conditions are _________________