ADVANCED TECHNOLOGY CORE LABORATORIES:
Antibody-based Proteomics Core
Request Form
Dan L. Dancun Cancer Center
Shixia Huang, Ph.D.,
Director
Alkek Building for Biomedical Research
R507
shixiah@bcm.edu
713 798 8722
Antibody Array Services
DLDCC Price*
Non-DLDCC Price*
RPPA Set Up Fee
$85
$95
RPPA Lysis Buffer
$34
$45
Core assisted preparation of tissue or cell lysates
(Includes processing, protein quantitation and
aliquoting)
RPPA with 180 validated antibodies*
(1440 lysate/slide format)
$25/sample
$100/sample
$0.64/lysate/antibody
$115/lysate for 180 antibodies
$2.56/lysate/antibody
$460.80/lysate for 180 antibodies
RPPA with 180 validated antibodies*
(2880 lysate/slide format)
$0.37/lysate/antibody
$66.60/lysate for 180 antibodies
$1.48/lysate/antibody
$266.40/lysate for 180 antibodies
Antibody aliquots for investigator immunoblot
validation of RPPA results (from antibody inventory
used for RPPA)
Test print
$40/antibody
$40/antibody
$10/sample
$40/sample
*number of total antibodies per RPPA run may vary
Dec 2012
Antibody Array Services
Protein labeling, purification, and QC (including
reagents, Sigma array platform)
Protein labeling, purification, and QC (including
reagents, ClonTech array platform)
DLDCC Price*
Non-DLDCC Price*
$270/sample
$318/sample
$295/sample
$347/sample
Array and labeling experiments: this service includes
time, array, reagents, labeling, purification, QC, and
cleanup etc
$1366/array (Clontech BD500),
$1574/array
(Sigma XPRESS 725) (two dyes), $1198/array
(Sigma 725) single dye
$864/array (Sigma, gene regulation array)
$1527 (Clontech)
$1724
(Sigma XPRESS 725) (two dyes) $1410/array
(Sigma 725) (single dye)
$1016 (Sigma, Gene regulation)
Array and labeling experiments: this service includes
time, array, reagents, labeling, purification, and
cleanup etc
Raybiotech >10 samples
Raybiotech, > 40 samples
$589/sample (Human L507, Raybiotech)
$538/sample (Mouse L308, Raybiotech)
$522/sample, human, $481/sample, mouse
$489/sample, human, $451/sample, mouse
$825/array/sample (2-8 arrays)
$716/array/sample (10 arrays)
$693/sample
$633/sample
$614/sample, $565/sample
$575 (human), $530 (mouse)
$1017/array/sample (2-8 arrays)
$803/array/sample (>10 arrays)
$1395/array/sample (2-8 arrays)
$1212/array/sample (10 arrays)
$1587/array/sample (2-8 arrays)
$1375/array/sample (10 arrays)
Array and labeling experiments* (Fullmoon Explorer
$1153/array/sample (2-8 arrays)
$907/array/sample (10 arrays)
array): this service includes time, array, reagents,
labeling, purification, QC, and cleanup etc
Array and labeling experiments* (Fullmoon P53
$854/array/sample (2-8 arrays)
$725/array/sample (10 arrays)
array): this service includes time, array, reagents,
labeling, purification, QC, and cleanup etc
Array and labeling experiments* (Fullmoon Apoptosis $815/array/sample (2-8 arrays)
$725/array/sample (10 arrays)
array): this service includes time, array, reagents,
labeling, purification, QC, and cleanup etc
$1344/array/sample (2-8 arrays)
$1070/array/sample (10 arrays)
Array and labeling experiments* (Fullmoon Phospho
Apoptosis array): this service includes time, array,
reagents, labeling, purification, QC, and cleanup etc
$949/array/sample (2-8 arrays)
$829/array/sample (10 arrays)
$1141/array/sample (2-8 arrays)
$992/array/sample (10 arrays)
Array scan
$30/array
$40/array
Array and labeling experiments* (Fullmoon Insulin
array): this service includes time, array, reagents,
labeling, purification, QC, and cleanup etc
Array and labeling experiments* (Fullmoon Phospho
Explorer array): this service includes time, array,
reagents, labeling, purification, QC, and cleanup etc
Array image analysis
$40/array
(For Full Moon, Sigma-Aldrich, and Raybiotech slides)
Array Image Analysis
$400/array
(For Invitrogen ProtoArray slides
Data processing tool from RayBiotech (optional
based on analysis need downstream)
Data Processing** using Raybiotech's tool,
Clontech's analysis tool, or excel fold calculation
$428/set of =< 4 arrays
$1003/array/sample (2-8 arrays)
$862/array/sample (10 arrays)
$1007/array/sample (2-8 arrays)
$862/array/sample (10 arrays)
$60/array
$500/array
$250 (Human L507)/project
$150 (Mouse L308)/project
$570/set of =< 4 arrays
*Please note this price is an estimate based on the protocol on time and reagent required at the time this estimate is made. Please
consult the
director for the actual price at the time of your project to be performed.
*We encourage PIs to work with a statistician to analyze the data. In the case you only have a small number of samples and would like to get a
quick fold difference using excel spread sheet or the companies' tools, we will process the data as requested.
Examples of Luminex Assay Services*
Analytes
DLDCC Price
Non-DLDCC Price
Luminex assays
Cat# RCYTO-80K-05, TNF-alpha, IL-1 beta, IL-10, GMCSF, VEGF
Cat# RCYTO-80K-PMX24, GM-CSF, GRO/KC, IFNg, IL1a, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL13, IL-17, IL-18, MCP-1, TNFa, G-CSF, IP-10, MIP-1a,
RANTES, Leptin, Eotaxin, VEGF
Cat# TGFB-64K-01, TGFB1
Cat# TGFB-64K-03, TGFB1, TGFB2, TGFB3
Plate reading and data exporting
varies
802-5500
855-6000
5
1334
1391
24
1
3
1-20 analytes/plate
3290
802
1149
$120
3363
855
1207
$145
Plate reading and data exporting
21 or more analytes/plate
$150
$180
*Price in this category is highly dependent on the reagent price. It will change when reagents are ordered at different times
*This form provides some examples. For other Luminex assays, please contact Core Director for estimate.
Sample preparation /processing
Cell pellet preparation from dishes
Sample preparation from pellets
Sample preparation from tissues
Protein assay (Bradford/BCA)
DLDCC Price
Non-DLDCC Price
$60/sample
$60/sample
$75/sample
$60/plate
$65/set of =<20 samples for first set of tubes,
$23 for each subsequent of tubes of the same
sample set
$70/sample
$70/sample
$90/sample
$70/plate
$80/set of =<20 samples for the first set of
tubes, $27 for each subsequent of tubes of
the same sample set
Sample processing: centrifugation and transferring
samples to plates from tubes (if we have tubes with
the exact amount we need)
$80/plate of <=40 samples for the first plate,
$23 to each subsequent plate of the same
sample set
$95/plate of =<40 samples for first plate and
$27 to each subsequent plate of the same
sample set
Plates (for users who aliquot their own samples
but don't have plates)
$4/plate
Sample processing: centrifugation, labeling tubes
and transferring to tubes
$5/plate
Dec-12
Self or Assisted Services
DLDCC Price*
Non-DLDCC Price*
$100/time
$15
$120
$20
$80
$80
$50
$80
$140
$80
$55
$100
$100
$70
$95
$160
$95
$80
$20
$25
Image analysis on protein arrays by users
Initial training (1-2 hours), 5 trainings are required within
two months to become an unassisated user
Using software/computer (per hour)*
Luminex plate reading and analysis
LPRS training (required prior to making any bookings)
Setup/Shut down instrument by Core
Plate reading and analysis by users (per hour)*
Plate reading - assisted by core (per hour)
Data Exporting-Cytokine Assays (per plate)
Data Exporting-Phosphoprotein Assays (per plate)
Use of Core Luminex Equipment (per plate)
Filter Plate (for users who need extra plate)
Exceeding the hour for 10 minutes will be counted for one hour