SOP Number: CP060.00
Title: Surgical Procedure for Placement of Gastric Catheter
Revision No: Replaces:
00
N/A
Author: Kristin Smith
I
Date in effect: Page:
7/15/2010
Page 1 of 4
MAL Director: Dr. Peggy Borum
FSHN Chair: Dr. Neil Shay
PURPOSE
The purpose of surgical procedure for placement of gastric catheter is to gain the ability to access
the stomach during the experiment. Sterile person will hand instruments to Dr. Borum as needed.
Sterile person must anticipate when instruments are needed.
II
SCOPE
This procedure provides instructions for the placement of gastric catheter.
III
RESPONSIBILITIES
It is the responsibility of Metabolic Assessment Laboratory personnel to follow this procedure.
It is the responsibility of supervisory personnel to ensure compliance with this procedure and to
train employees and students responsible for performing this procedure. Students will report
accidents to the principal investigators immediately.
IV
REFERENCES
N/A
V
REAGENTS AND MATERIALS
V.A. 20-gauge leur adapter.
V.B. 1 cc syringe filled with 0.4 cc lidocaine (2%).
V.C. Babcock.
V.D. Scissors (small).
V.E. 4-0 dexon to approximate muscle.
V.F. Hemostats.
V.G. 16-gauge male locking leur adapter.
V.H. 1cc syringe of 250 mg cefazolin
V.I. 3-0 running nylon to approximate skin.
V.J. Gastric catheter.
V.K. Toothed and non-toothed forceps.
V.L. Needle holder.
V.M. Scalpel handle.
V.N. #10 scalpel blade.
V.O. Bioclusive Tape Pack.
VI
EQUIPMENT
N/A
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SOP Number: CP060.00
Title: Surgical Procedure for Placement of Gastric Catheter
Revision No:
00
VII
Replaces:
N/A
Date in effect:
7/15/2010
Page:
Page 2 of 4
SAFETY PRECAUTIONS
VII.A. Members of the MAL have been trained extensively in the procedures described in this
SOP.
VII.B. Members of the MAL have completed animal contact training in order to work in the
PNICU.
VIII
DEFINITIONS
VIII.A. Standard Operating Procedure (SOP) – Standard Operating Procedure is a document that
provides instructions for completing a specific task in the lab.
VIII.B. Metabolic Assessment Laboratory (MAL) – The Metabolic Assessment Laboratory is the
laboratory that will use this SOP.
VIII.C. Piglet Neonatal Intensive Care Unit (PNICU) – The PNICU is a unit where the piglet is
monitored by 24 hour care and routine check-up parameters using PNICU SOPs
conducted by the MAL.
VIII.D. Sterile Person—The Sterile Person is designated to handle all sterile instruments and
materials that will be used inside the piglet. The Sterile Person also passes instruments to
the surgeon.
IX
PROCEDURE
IX.A. Gastric catheter preparation
IX.A.1. Insert a 20-gauge luer stub adapter into gastric catheter. Refer to SOP# CP041.00
and CP# CP051.00 on how to set up the catheter.
IX.B. Make the gastric incision
IX.B.1. Expose the incision site.
IX.B.2. Inject 0.4 cc lidocaine (lidocaine HCL, USP, 1%) at site of incision
subcutaneously.
IX.B.3. Make a 2-4 cm abdominal incision above the stomach using the scalpel and
forceps.
IX.B.4. Open up the muscle carefully using a curved hemostat to expose the stomach.
IX.B.5. Identify the stomach and deliver it to the incision with Babcock forceps.
IX.B.6. If necessary, Dr. Borum will gently remove the peritoneal membrane using nontoothed forceps and scissors, while Sterile person holds the Babcock.
IX.B.7. Sew in double purse-string sutures (4-0 dexon PDS II) on the surface of the
stomach using a needle holder.
IX.B.8. Insert a catheter into the center of the purse-strings via a small puncture made by
a small cut with scissors and enlarged with hemostats. At this point, the Babcock
is replaced with a non-toothed forceps.
IX.B.9. Secure the inner purse-string suture around the catheter on the distal side of the
collar from the tip. Sterile person pushes down slightly on the stomach, while
Dr. Borum secures the outer purse-string.
IX.B.10. Crosstie the 4 purse-strings three times.
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SOP Number: CP060.00
Title: Surgical Procedure for Placement of Gastric Catheter
Revision No:
00
Replaces:
N/A
Date in effect:
7/15/2010
Page:
Page 3 of 4
IX.C. Closing the incision site
IX.C.1. Use an antibiotic such as a 1cc syringe of 250 mg cefazolin to irrigate the
operation site
IX.C.2. Close the stomach area automatically in layers with 4-0 dexon sutures and the
skin approximated with 3-0 running nylon sutures.
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SOP Number: CP060.00
Title: Surgical Procedure for Placement of Gastric Catheter
Revision No:
00
X
Replaces:
N/A
Date in effect:
7/15/2010
Page:
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ATTACHMENTS
X.A. For a list of materials and their locations, refer to SOP# CG098.00.
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