Review Key Day 2 Cell Division and Genetics 1.The DNA must replicate before mitosis in order to have two identical sets of DNA, one for each daughter cell 2.The cell will complete mitosis when making ____somatic/body/diploid____ cells. 3.The cell will complete meiosis when making __gamete/sex/haploid_ cells. 4. List four differences between mitosis and meiosis: 1. 2. 3. 4. Mitosis creates diploid cells while meiosis creates haploid cells Mitosis creates genetically identical cells while meiosis creates genetically unique cells Mitosis is used for growth, repair and healing while meiosis creates sex cells Meiosis is two sets of divisions while mitosis is one set of divisions 5. Summarize how sexual reproduction, which includes meiosis and fertilization, affects genetic variation within an offspring. Meiosis makes genetically different gametes because crossing-over occurs. When fertilization occurs, a genetically unique egg cell is fused with a genetically unique sperm cell to create a unique zygote 6. Mitosis phases Phase What happens in the phase Interphase “I’m working” – cell does its jobs like making proteins, repairing organelles, and growing Prophase Metaphase “Pieces of DNA” – DNA coils into chromosomes to prepare for cell division/nucleus disappears “Middle” – Chromosomes line up at the middle of the cell Anaphase “Away” – Chromosomes are pulled away from one another Telophase “Twin Nuclei form” – the nuclei reform around the separated sets of DNA 9. What are the three components of a nucleotide? 1 sugar + 1 phosphate + 1 base 10. Draw a picture of the structure of DNA that (at least) includes the terms: base, 1 sugar, phosphate, nucleotide, and helix. 11. Describe gel electrophoresis using the following terms: electrophoresis, agarose gel, DNA bands, banding pattern, lane, DNA fragment, common ancestry, relatedness Gel electrophoresis separates DNA fragments using electricity. DNA fragments move through an agarose gel and banding patterns are viewed to see relatedness between individuals. Common ancestry, paternity, and crime scene evidence can all be determined using gel electrophoresis. 12. What do the bands in the gel pattern represent? What causes some bands to move further than other? DNA segments – short DNA segments move further than long segments because they fit easily through the agarose gel 13. RNA/ DNA Comparison: Fill in the chart Characteristic DNA Sugar present Deoxyribose RNA Ribose Number of strands 2 1 Location(s) It Can Be Found Nucleus of eukaryotes/cytoplasm of prokaryotes as a nucleoid Nucleus or cytoplasm Used in ribosomes Function(s) Holds all genetic information for an organism Helps create a protein from a gene sequence 13. Mutations: Mutations and crossovers create different genes and gene sequences. Explain how mutations are an important and normal part of sexual reproduction. Crossing-over during meiosis 1 swaps genes on DNA to create variation in the gametes. This helps ensure that all offspring will be genetically different from their parents and from one another 14. Protein Synthesis Definitions Word Definition/Picture Codon Series of 3 bases on messenger RNA – each codon indicates 1 amino acid needed to build a protein Nucleotide 1 sugar + 1 phosphate + 1 base/building blocks of DNA and RNA Replication Copying an identical strand of DNA – occurs in the nucleus before mitosis and meiosis (REMEMBER: Crossing over during meiosis changes the DNA in each gamete!!!) Anticodon A 3 base sequence on tRNA that is the opposite of a codon Clone genetically identical offspring mRNA Messenger RNA – carries the copied DNA information to make a protein tRNA Transfer RNA – brings amino acids to ribosomes in order to build a protein 15.What happens during transcription? DNA information to make a protein (1 gene) is copied on to mRNA in the nucleus 16.What happens during translation? mRNA brings the gene information to the ribosome. At the ribosome, mRNA is read one codon at a time so that tRNA can bring the correct amino acid sequence to form a protein 17. What is a chain of amino acids called? protein 18.Genetics Definitions Word Definition Allele Any variation on a trait, written as a letter Autosome A body chromosome/identical in males and females/humans have 22 pairs of autosomes Dominant An allele that is the phenotype when one or two copies are present Gene Genotype DNA information to produce a protein The two letter genetic sequence for a trait Heterozygous Aa – dominant trait expressed Homozygous AA or aa – having two identical alleles for a given trait Phenotype The physical appearance of an organism Recessive Lowercase letter, the allele the is expressed only when an individual is homozygous recessive for a trait Females – XX males – XY Sex-chromosome Complete the following punnett squares. Give the genotype and phenotype of each. 19. Monohybrid 20. Sex-linked Cross a heterozygous right handed person with a left handed person. Right is dominant over left. Cross a female who is a carrier for hemophilia with a hemophiliac male. 21.Answer the following questions. In Pisum sativum, a pea plant, the allele for purple flower (P) is dominant over the allele for white flowers (p). A cross between two purple-flowered plants in both purple-flowered and white-flowered offspring, as shown in the table below. RESULTS OF PEA PLANT CROSS Flower Number of Plants Purple 103 White 35 On a piece of paper, do the following: Draw a Punnett Square that shows the cross between the two purple-flowered parent plants described above. When writing the allele pairings, underline all lowercase letters (p). Fill in the genotypes of the offspring on the Punnett Square. Make a key to indicate which genotype produces which flower color. Give the ratio of flower colors that can be expected from the cross. Explain how the data in the table and in the Punnett square helped you determine the ratio. 22. A genetics study was conducted that crossed two red-flowered plants. The next generation was a mixture of redflowered and white-flowered offspring. Which of these represents those of the parent generation? A) rr and rr B) Rr and Rr C) RR and rr D) RR and RR