Table S1 Matrix of Pearson product-moment correlations among environmental predictor
variables (all except pH log10 transformed).
Temperature
Conductivity pH
Conductivity
0.22
pH
0.22
-0.43
Dissolved oxygen
0.60
-0.25
0.24
Discharge
0.24
0.30
0.20
Dissolved oxygen
0.33
Table S2 Mean (±1S.E.) and 25th and 75th percentiles of the water quality variables at each of
the three main channel and four tributary sites sampled between October 2005 and December
2009 in the Blackwood River study area.
Site
Main channel
Denny Rd
Milyeannup Pool
Jalbarragup
Tributaries
Rosa Brook
Layman Brook
Milyeannup Brook
McAtee Brook
Temperature
(°C)
Conductivity
(µS.cm-1)
Dissolved
oxygen (mg.L-1)
pH
17.17 (0.43)
13.30-21.10
17.64 (0.47)
13.98-21.55
16.88 (0.51)
12.73-20.53
3563.9 (155.5)
2512-4660
4458.6 (127.3)
3668.8-5364.8
4954.7 (174.6)
3755-5925
8.46 (0.19)
7.47-9.35
8.00 (0.18)
7.06-9.06
8.27 (0.34)
6.81-9.54
7.18 (0.05)
6.98-7.32
7.20 (0.08)
6.89-7.51
7.33 (0.06)
7.10-7.54
13.80 (0.35)
12.00-16.00
13.89 (0.42)
12.25-15.03
14.73 (0.26)
12.90-16.80
15.48 (0.45)
12.80-19.20
496.82 (24.00)
361.0-535.9
285.5 (7.88)
238.3-316.4
362.23 (8.85)
292.9-436.2
496.22 (21.22)
363.70-585
6.04 (0.12)
6.52-10.02
9.18 (0.47)
8.59-10.48
7.98 (0.22)
6.65-9.30
9.17 (0.33)
8.08-10.40
6.04 (0.12)
5.64-6.58
6.67 (0.09)
6.10-6.95
6.11 (0.06)
5.73-6.45
6.13 (0.08)
5.92-6.32
Table S3 Prevalence of native freshwater fishes (% occurrence at all sites in all months
sampled, total samples n = 218) in the Blackwood River study area between October 2005
and December 2009. Mean (±1S.E.), median and range of environmental variables at all sites
at each time the species were recorded are also presented.
Species
Variable
Prevalence overall
(% )
Prevalence
tributaries (%)
Prevalence main (%)
Temperature (°C)
Conductivity (µS.cm1
)
pH
Dissolved oxygen
(mg.L-1)
Turbidity (NTU)
Instantaneous
discharge (l.s-1)
Mean
(±1S.E.)
Median
Maximum
Minimum
Mean
(±1S.E.)
Median
Maximum
Minimum
Mean
(±1S.E.)
Median
Maximum
Minimum
Mean
(±1S.E.)
Median
Maximum
Minimum
Mean
(±1S.E.)
Median
Maximum
Minimum
Mean
(±1S.E.)
Median
Maximum
Minimum
N. balstoni
N. vittata
B. porosa
38.5
67.4
66.5
G. occidentalis
92.2
G. munda
16.5
T. bostocki
65.6
42.6
82.6
85.7
95.2
32.4
30.5
34.5
16.56
(0.41)
16.6
24.73
8.33
2103.3
(222.8)
560
6920
190
6.55
(0.07)
6.41
8.22
5.29
8.07
(0.20)
7.79
14.63
3.47
2.57
(0.17)
2.15
6.96
0.79
1843.2
(662.9)
336.7
41399.8
3.7
54.0
16.44
(0.32)
16.2
24.77
8.33
1876.0
(173.6)
457
10220
190
6.51
(0.06)
6.41
8.27
3.86
8.32
(0.15)
8.27
16.04
3.66
2.64
(0.12)
2.15
6.96
0.65
2887.2
(718.0)
350.2
46517.3
3.7
49.5
16.11
(0.32)
15.52
24.77
8.33
1788.4
(178.3)
442
10220
190
6.56
(0.07)
6.49
8.27
3.86
8.39
(0.18)
8.27
17.47
1.61
2.47
(0.13)
1.98
6.96
0.65
2319.0
(624.6)
221.67
42795.7
0.1
90.3
15.97
(0.27)
15.33
24.77
8.33
2200.6
(151.9)
800
10220
190
6.71
(0.06)
6.78
8.34
3.86
8.51
(0.14)
8.39
17.47
3.44
2.47
(0.10)
2.01
6.96
0.65
3433.7
(664.9)
349.47
46517.3
2.0
2.7
15.25
(0.54)
15.6
21.43
8.40
780.0
(188.8)
433
4804
273
5.97
(0.12)
6.06
7.51
3.86
8.36
(0.39)
8.20
16.04
4.65
2.43
(0.16)
2.16
3.89
1.32
499.4
(247.7)
74.0
7862.0
3.7
99.0
16.94
(0.34)
16.0
24.77
8.33
3256.3
(174.6)
3543
10220
190
7.04
(0.05)
7.15
8.45
5.44
8.30
(0.15)
8.29
17.47
3.44
2.91
(0.13)
2.39
6.96
0.91
5074.7
(926.5)
597.9
46517.3
3.7
Fig. S1 Mean number of Galaxias occidentalis moving in the tributary and main channel sites
of the Blackwood River, south-western Australia, between October 2005 and December 2009.
NF indicates that the system was not flowing and therefore was not sampled.
Downstream
Upstream
Rosa Brook
400
300
200
100
NF NF
0
NF NF
NF NF
NF NF
Layman Brook
500
400
300
200
100
NF NF
0
NF
NF NF NF
NF NF NF NF
NF NF NF
NF
Milyeannup Brook
300
200
0
McAtee Brook
200
150
100
50
NF NF
0
NF NF
NF NF NF
NF NF NF
Blackwood River - Denny Rd
1200
900
600
300
NF
0
Blackwood River - Milyeannup Pool
800
600
400
200
0
NF
NF NF
NF
Blackwood River - Jalbarragup
200
150
100
50
0
NF NF
NF NF NF
NF
NF NF
Oc
t
No 05
v
De 05
c
Fe 05
b
M 06
ar
Ju 06
n
Au 06
g
Se 06
p
M 06
ar
M 07
ay
Ju 07
n
Au 07
g
Se 07
p
Oc 07
t
No 07
v
De 07
c
M 07
ar
Ap 08
Ju r 08
ne
Au 08
g
Se 08
p
No 08
v
De 08
c
M 08
ar
M 09
ay
Ju 09
n
Se 09
p
Oc 09
t
No 09
v
De 09
c
09
Mean number of fish
100
Month
Fig. S2 Length-frequency distributions of Galaxias occidentalis in Milyeannup Brook. N.B.
Distributions are differentiated by upstream and downstream movement.
2005
140
June
20
10
20
0
0
0
November
August
40
20
5
0
0
0
December
September
10
5
20
0
0
0
2006
10
February
October
60
30
5
0
0
0
March
November
10
5
5
0
0
0
June
80
December
20
10
5
0
0
0
August
2008
20
March
10
5
0
0
0
September
April
40
20
10
0
0
0
2007
March
August
40
20
10
0
0
0
May
10
September
10
5
0
20 40 60 80 100 120 140 160
November
December
10
5
0
0
October
20
5
20
September
20
20
10
August
10
20
40
June
10
40
40
May
10
5
March
2009
10
5
10
2009
40
10
Downstream
Upstream
December
10
50
20
November
40
70
100
Number of fish
October
0
0
20 40 60 80 100 120 140 160
Total length (mm)
0
20 40 60 80 100 120 140 160
Fig. S3 Length-frequency distributions of Galaxias occidentalis in Rosa Brook. N.B.
Distributions are differentiated by upstream and downstream movement.
2005
10
October
5
20
0
0
0
November
August
10
5
10
0
0
0
December
September
10
5
5
0
0
0
2006
10
February
5
NF
0
March
10
5
NF
0
June
10
October
20
5
0
0
November
5
0
0
December
40
20
5
0
0
0
August
5
2008
10
March
5
0
September
5
0
0
2007
10
March
5
NF
0
May
20
10
April
10
20
0
0
20 40 60 80 100 120 140 160
June
August
September
10
0
October
10
5
NF
August
40
0
5
0
0
September
60
November
10
20
30
NF
NF
5
NF
0
40
May
10
5
10
NF
10
10
March
2009
10
10
20
2009
10
60
Downstream
Upstream
December
20
10
120
November
40
5
20
Number of fish
June
10
December
10
5
0
0
0
20 40 60 80 100 120 140 160
Total length (mm)
0
20 40 60 80 100 120 140 160
Fig. S4 Mean number of Galaxiella munda moving in the tributary sites of the Blackwood
River, south-western Australia, between October 2005 and December 2009. NF indicates that
the system was not flowing and therefore was not sampled.
Downstream
Upstream
Rosa Brook
10
5
0
NF NF
NF NF
10
NF NF
NF NF
Layman Brook
0
NF NF
NF
NF NF NF
60
NF NF NF NF
NF NF NF
Milyeannup Brook
50
40
30
20
10
0
McAtee Brook
10
5
0
NF NF
NF NF
NF NF NF
NF NF NF
O
ct
No 05
v
De 05
c
Fe 05
b
M 06
ar
Ju 06
n
Au 06
g
Se 06
p
M 06
ar
M 07
ay
Ju 07
n
Au 07
g
Se 07
p
O 07
ct
No 07
v
De 07
c
M 07
ar
Ap 08
Ju r 08
ne
Au 08
g
Se 08
p
No 08
v
De 08
c
M 08
ar
M 09
ay
Au 09
g
Se 09
p
O 09
ct
No 09
v
De 09
c
09
Mean number of fish
5
Month
NF
Fig. S5 Length-frequency distributions of Galaxiella munda in Milyeannup Brook. N.B.
Distributions are differentiated by upstream and downstream movement.
2005
10
October
5
5
0
0
0
November
August
10
5
5
0
0
0
December
10
September
10
5
5
0
0
0
2006
February
October
10
5
5
0
0
0
March
November
40
20
5
0
0
0
June
December
10
5
5
0
0
0
August
2008
10
March
5
5
0
0
0
September
April
10
5
5
0
0
0
2007
March
August
10
5
5
0
0
0
May
September
10
5
10
20
30
40
50
60
November
December
5
0
0
October
10
5
0
September
10
5
10
August
10
5
10
June
10
5
10
May
10
5
10
2009
10
5
10
March
10
5
10
2009
10
5
10
December
10
10
Downstream
Upstream
November
10
5
20
Number of fish
June
10
0
0
10
20
30
40
Total length (mm)
50
60
0
10
20
30
40
50
60
Fig. S6 Mean number of Nannatherina balstoni moving in the tributary and main channel
sites of the Blackwood River, south-western Australia, between October 2005 and December
2009. NF indicates that the system was not flowing and therefore was not sampled.
NF NF
NF NF
NF NF
NF NF
Layman Brook
100
80
60
40
20
0
NF NF
NF
NF NF NF
NF NF NF NF
NF NF NF
NF
Milyeannup Brook
4000
3000
2000
800
400
0
McAtee Brook
100
80
60
40
20
0
NF NF
NF NF
NF NF NF
200
NF NF NF
Blackwood River - Denny Rd
150
100
50
NF
0
Blackwood River - Milyeannup Pool
3000
2500
2000
200
100
NF NF
0
NF
100
NF
Blackwood River - Jalbarragup
80
60
40
20
0
NF
NF
NF NF NF
NF
NF NF
Oc
t
No 05
v
De 05
c
Fe 05
b
M 06
ar
Ju 06
n
Au 06
g
Se 06
p
M 06
ar
M 07
ay
Ju 07
n
Au 07
g
Se 07
p
Oc 07
t
No 07
v
De 07
c
M 07
ar
Ap 08
Ju r 08
ne
Au 08
g
Se 08
p
No 08
v
De 08
c
M 08
ar
M 09
ay
Ju 09
n
Se 09
p
Oc 09
t
No 09
v
De 09
c
09
Mean number of fish
Downstream
Upstream
Rosa Brook
100
80
60
40
20
0
Month
Fig. S7 Length-frequency distributions of Nannatherina balstoni in Milyeannup Brook. N.B.
Distributions are differentiated by upstream and downstream movement.
2005
10
June
40
20
20
0
0
0
November
August
80
40
20
0
0
0
December
60
September
10
5
20
0
0
0
2006
February
October
10
5
5
0
0
0
March
November
60
30
10
0
0
0
June
December
20
10
5
0
0
0
August
2008
10
March
5
5
0
0
0
September
April
20
10
20
0
0
0
2007
March
August
10
5
20
0
0
0
May
September
10
5
20
40
60
80
100
November
December
20
0
0
October
40
5
0
September
40
5
10
August
40
5
10
June
10
20
10
May
10
5
40
2009
20
5
10
March
10
5
10
2009
40
30
10
December
40
70
Downstream
Upstream
November
40
5
140
Number of fish
October
0
0
20
40
60
80
Total length (mm)
100
0
20
40
60
80
100
Fig. S8 Mean number of Nannoperca vittata moving in the tributary and main channel sites of
the Blackwood River, south-western Australia, between October 2005 and December 2009.
NF indicates that the system was not flowing and therefore was not sampled.
Downstream
Upstream
Rosa Brook
500
400
300
200
100
NF NF
0
NF NF
NF NF
NF NF
Layman Brook
100
80
60
40
20
NF NF
0
NF
NF NF NF
NF NF NF NF
NF NF NF
NF
Milyeannup Brook
300
200
100
McAtee Brook
100
80
60
40
20
NF NF
0
NF NF
NF NF NF
600
500
400
300
200
100
0
Blackwood River - Denny Rd
600
500
400
300
200
100
0
Blackwood River - Milyeannup Pool
NF NF NF
NF
NF NF
NF
NF
Blackwood River - Jalbarragup
100
80
60
40
20
0
NF NF
NF NF NF
NF
NF NF NF
O
ct
No 05
v
De 05
c
Fe 05
b
M 06
ar
Ju 06
n
Au 06
g
Se 06
p
M 06
ar
M 07
ay
Ju 07
n
Au 07
g
Se 07
p
O 07
ct
No 07
v
De 07
c
M 07
ar
Ap 08
r
Ju 08
n
Au 08
g
Se 08
p
No 08
v
De 08
c
M 08
ar
M 09
ay
Ju 09
n
Se 09
p
O 09
ct
No 09
v
De 09
c
09
Mean number of fish
0
Month
Fig. S9 Length-frequency distributions of Nannoperca vittata in Milyeannup Brook. N.B.
Distributions are differentiated by upstream and downstream movement.
2005
10
June
10
5
10
0
0
0
November
August
10
5
5
0
0
0
December
20
September
10
5
10
0
0
0
2006
February
October
10
5
5
0
0
0
March
November
10
5
5
0
0
0
June
10
December
10
5
5
0
0
0
August
2008
10
March
5
5
0
0
0
September
April
10
5
5
0
0
0
2007
March
August
10
5
5
0
0
0
May
September
10
5
20
40
60
80
December
5
0
0
November
10
5
0
October
10
5
10
September
10
5
10
August
10
5
10
June
10
5
10
May
10
5
March
2009
10
5
10
2009
20
10
10
December
10
5
Downstream
Upstream
November
20
5
10
Number of fish
October
0
0
20
40
60
Total length (mm)
80
0
20
40
60
80
Fig. S10 Length-frequency distributions of Nannoperca vittata in Rosa Brook. N.B.
Distributions are differentiated by upstream and downstream movement, NF indicates the
system was not flowing and therefore was not sampled.
2005
10
October
5
20
0
0
0
November
August
10
5
20
0
0
0
December
80
September
10
5
10
0
0
0
2006
February
5
NF
0
March
10
NF
June
40
5
0
0
November
5
0
0
December
40
20
5
0
0
0
August
2008
10
10
March
September
5
0
0
2007
10
March
NF
0
May
10
0
August
0
0
20
40
5
20
0
0
September
80
December
60
30
0
60
November
40
40
NF
October
20
NF
80
5
September
10
10
5
August
0
April
10
5
June
20
NF
0
10
NF
40
5
0
May
10
20
20
NF
10
20
March
2009
10
20
40
5
0
October
40
2009
20
40
10
December
40
5
Downstream
Upstream
November
40
5
10
Number of fish
June
10
0
0
20
40
60
Total length (mm)
80
0
20
40
60
80
Fig. S11 Mean number of Bostockia porosa moving in the tributary and main channel sites of
the Blackwood River, south-western Australia, between October 2005 and December 2009.
NF indicates that the system was not flowing and therefore was not sampled.
Downstream
Upstream
Rosa Brook
200
150
100
50
NF NF
0
NF NF
NF NF
NF NF
Layman Brook
400
300
200
100
NF NF
0
NF
NF NF NF
NF NF NF NF
NF
NF NF NF
Milyeannup Brook
200
150
100
0
McAtee Brook
100
80
60
40
20
0
NF NF
NF NF
NF NF NF
700
600
500
400
300
200
100
0
Blackwood River - Denny Rd
100
Blackwood River - Milyeannup Pool
NF NF NF
NF
80
60
40
20
0
NF NF
NF
NF
Blackwood River - Jalbarragup
100
80
60
40
20
0
NF NF
NF NF NF
NF
NF NF
Oc
t
No 05
v
De 05
c
Fe 05
b
M 06
ar
Ju 06
n
Au 06
g
Se 06
p
M 06
ar
M 07
ay
Ju 07
n
Au 07
g
Se 07
p
Oc 07
t
No 07
v
De 07
c
M 07
ar
Ap 08
r
Ju 08
n
Au 08
g
Se 08
p
No 08
v
De 08
c
M 08
ar
M 09
ay
Ju 09
n
Se 09
p
Oc 09
t
No 09
v
De 09
c
09
Mean number of fish
50
Month
Fig. S12 Length-frequency distributions of Bostockia porosa in Rosa Brook. N.B.
Distributions are differentiated by upstream and downstream movement. NF indicates that the
system was not flowing and therefore was not sampled.
2005
10
October
5
5
0
0
0
November
August
10
5
5
0
0
0
December
September
10
5
5
0
0
0
2006
10
February
5
NF
0
March
10
5
NF
0
June
10
October
10
5
0
0
November
5
0
0
December
20
10
5
0
0
0
August
5
2008
10
March
5
0
September
5
0
0
2007
10
March
5
NF
0
May
10
5
April
10
5
0
0
20 40 60 80 100 120 140 160
June
August
September
10
0
October
10
5
NF
August
10
0
5
0
0
September
10
November
10
5
5
NF
NF
5
NF
0
10
May
10
5
10
NF
10
5
March
2009
10
5
10
2009
10
80
Downstream
Upstream
December
10
5
160
November
10
5
10
Number of fish
June
10
December
40
20
0
0
0
20 40 60 80 100 120 140 160
Total length (mm)
0
20 40 60 80 100 120 140 160
Fig. S13 Length-frequency distributions of Bostockia porosa in Layman Brook. N.B.
Distributions are differentiated by upstream and downstream movement. NF indicates that the
system was not flowing and therefore was not sampled.
2005
10
October
June
10
5
5
20
0
0
0
November
10
August
20
10
5
0
0
0
December
September
10
5
5
0
0
0
2006
10
February
5
NF
0
March
10
Number of fish
5
NF
0
June
10
5
October
10
5
0
0
November
5
0
0
December
5
NF
0
August
10
5
NF
0
2008
10
March
September
5
April
10
5
0
2007
March
5
NF
0
May
10
5
August
10
0
0
20 40 60 80 100 120 140 160
June
August
10
0
September
10
0
October
10
0
10
0
0
September
10
November
20
5
5
NF
NF
5
NF
0
10
May
5
NF
0
10
NF
5
5
0
March
2009
10
20
10
2009
10
5
40
NF
10
100
Downstream
Upstream
December
10
5
200
November
40
December
10
5
0
NF
0
0
20 40 60 80 100 120 140 160
Total length (mm)
0
20 40 60 80 100 120 140 160