GENETICS PRACTICE REVIEW - MATCHING
Match each item with the correct statement below.
a. template strand
i. single-stranded binding proteins
b. ligase
j. leading strand
c. primase
k. gyrase
d. DNA polymerase I
l. helicase
e. lagging strand
m. annealing
f. replication fork
n. mitosis
g. semi-conservative replication
o. DNA polymerase III
h. Okazaki fragment
____ 38. process which results in each new DNA molecule consisting of one parental strand and one newly
synthesized strand
____ 39. unwinds DNA
____ 40. removes RNA primers
____ 41. new DNA strand synthesized in fragments
____ 42. division of a nucleus to form two daughter nuclei
____ 43. short lengths of DNA produced during synthesis of lagging strand
____ 44. keep newly separated strands of DNA apart
____ 45. new DNA strand which is synthesized continuously
____ 46. links sugars and phosphates together
____ 47. area where DNA polymerase is bound to unwound DNA
____ 48. DNA strand which directs synthesis of a complementary strand
____ 49. builds RNA primers
____ 50. relieves tension in DNA during unwinding
____ 51. pairing of complementary strands of DNA due to hydrogen bonding
____ 52. responsible for building new DNA strands during replication
Match each item with the correct statement below.
a. SINES
e. pseudogenes
b. LINES
f. nucleosomes
c. telomeres
g. centromeres
d. histone
____
____
____
____
____
____
____
44.
45.
46.
47.
48.
49.
50.
DNA sequences homologous with genes that are never transcribed.
noncoding DNA at the end of chromosomes.
groups of eight proteins enveloped by coiled DNA.
positively charged protein.
points of contact of two replicated chromosome strands.
repeated DNA sequences about 5000–7000 base pairs long.
repeated DNA sequences about 300 base pairs long.
Match each item with the correct statement below.
a. plasmid
e.
b. competent cell
f.
c. ligase
g.
d. host cell
h.
copy number
vector
cloned DNA
recombinant DNA
____ 60. a fragment of DNA, many copies of which have been produced
____ 61. a circular piece of DNA that is able to enter and leave bacterial cells
____ 62. a cell that has taken up a plasmid or virus
____ 63. a bacterial cell that readily takes up foreign DNA
____ 64. joins cut ends of DNA together
____ 65. containing sequences from more than one source
____ 66. number of copies of a plasmid in a bacterial cell
____ 67. vehicle by which DNA may be introduced into bacterial cells
Modified True/False
Indicate whether the sentence or statement is true or false. If false, change the identified word or phrase to make the
sentence or statement true.
____ 1. During the polymerase chain reaction, RNA primers are used that are complementary to part of the DNA
to be copied. These primers act as starting points for Taq polymerase, which builds the new DNA strands
in the 5'–3' direction. ______________________________
____
2. It is important to select a restriction nutrient that does not cut in the plasmid's origin of replication,
otherwise the plasmid will fail to replicate properly. _________________________
____
3. Cellulose ingested by cows is held in the rumen for many hours while it is digested by symbiotic bacteria
and protists. Periodically, it is returned to the mouth to be ground up and in order that alkaline saliva may
neutralize stomach acids. This complex and efficient process is an example of biotechnology.
_____________________________________________
____
4. Some restriction endonucleases make zigzag cuts in double-stranded DNA leaving blunt ends.
______________________________
____
5. You have a DNA sequence three thousand base pairs long, which you are going to cut using a particular
restriction endonuclease. The longer the recognition site of this enzyme, the more fragments you would
expect after digestion. _____________________________________________
____
6. Gel electrophoresis separates DNA fragments on the basis of their size. _________________________
____
7. During gel electrophoresis, DNA fragments migrate through the gel at a rate that is inversely
proportional to the logarithm of their size. _________________________________________
____
8. If a very dilute agarose gel is used to separate DNA fragments, the fragments will take a short time to
cross the gel. ______________________________
____
9. When Escherichia coli cells were engineered to produce the drug somatropin, the lac promoter was
included in the plasmid with which the bacteria were transformed, in order that the production of
somatropin might be controlled. This control is exercised using isopropyl thiogalactoside (IPTG), which
is an natural inducer of the lac promoter. When IPTG is absent, there is no somatropin production. As
well, when IPTG is present, production of the drug proceeds.
_____________________________________________
____ 10. Prior to the use of Taq polymerase in the polymerase chain reaction, other DNA polymerases from
organisms living at cooler temperatures were sometimes utilized. However, since most polymerases
denature at temperatures above approximately 37ºC, the reaction had to be cooled down much more after
the annealing of the primers before the polymerase could be added. The use of Taq polymerase has made
PCR much faster, as well as much cheaper. _________________________
____ 11. If only a small or degraded sample of DNA is available, PCR analysis is used because it has the capacity
to amplify the genetic material available. ___________________________________
____ 12. Antisense mRNA oligonucleotides are complementary to the mRNA molecules they deactivate. They are,
therefore, identical to the template strand of the DNA which has been transcribed to create the mRNA.
_________________________________________
MATCHING
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
52.
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
G
L
D
E
N
H
I
J
B
F
A
C
K
M
O
REF:
REF:
REF:
REF:
REF:
REF:
REF:
REF:
REF:
REF:
REF:
REF:
REF:
REF:
REF:
K/U
K/U
K/U
K/U
K/U
K/U
K/U
K/U
K/U
K/U
K/U
K/U
K/U
K/U
K/U
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
4.3
4.3
4.3
4.3
4.3
4.3
4.3
4.3
4.3
4.3
4.3
4.3
4.3
4.3
4.3
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
MG1.02
MG1.02
MG1.02
MG1.02
MG1.02
MG1.02
MG1.02
MG1.02
MG1.02
MG1.02
MG1.02
MG1.02
MG1.02
MG1.02
MG1.02
E
C
F
D
G
B
A
REF:
REF:
REF:
REF:
REF:
REF:
REF:
K/U
K/U
K/U
K/U
K/U
K/U
K/U
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
5.8
5.8
5.8
5.8
5.8
5.8
5.8
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
MG1.01
MG1.01
MG1.01
MG1.01
MG1.01
MG1.01
MG1.01
G
A
D
B
C
H
E
F
REF:
REF:
REF:
REF:
REF:
REF:
REF:
REF:
K/U
K/U
K/U
K/U
K/U
K/U
K/U
K/U
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
OBJ:
6.1
6.1
6.1
6.1
6.1
6.1
6.1
6.1
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
LOC:
MG1.05
MG1.06
MG1.06
MG1.06
MG1.06
MG1.06
MG1.06
MG1.06
MATCHING
44.
45.
46.
47.
48.
49.
50.
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
MATCHING
60.
61.
62.
63.
64.
65.
66.
67.
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
ANS:
Practice Test Chapter 6 Biotechnology
Answer Section
MODIFIED TRUE/FALSE
1. ANS: F, DNA primers are used
REF: K/U
OBJ: 6.3
2. ANS: F, restriction enzyme
LOC: MG1.06
REF: I
OBJ: 6.1
LOC: MG1.06
3. ANS: F, an example of a naturally evolved process
REF: MC
OBJ: 6.1
4. ANS: F, leaving sticky ends
LOC: MG1.05
REF: K/U
OBJ: 6.1
LOC: MG1.06
5. ANS: F, the shorter the recognition site of this enzyme
6.
7.
8.
9.
REF:
ANS:
LOC:
ANS:
LOC:
ANS:
LOC:
ANS:
REF:
10. ANS:
LOC:
11. ANS:
LOC:
12. ANS:
K/U
OBJ: 6.1
LOC:
T
REF:
MG1.05
T
REF:
MG1.05
T
REF:
MG1.05
F, artificial inducer of the lac promoter
MG1.06
K/U
OBJ: 6.1
K/U
OBJ: 6.1
K/U
OBJ: 6.1
I
OBJ: 6.2
LOC: MG1.05
T
REF: I
MG1.06
T
REF: K/U
MG1.05
F, not identical to the template strand
REF: K/U
OBJ: 6.4
LOC: MG1.05
OBJ: 6.3
OBJ: 6.3