Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
TABLES - Table number corresponds to section in TEXT
Table Ia
Table Ib
Table Ic
Common conditions by age
Common conditions or phenotypes by strain
Some immune relevant genotypes (in common strains)
Page 2
Table Id
Table Ie
Table If
Table Ig
Table Ih
Table Ii
Immunodeficient mice: Properties of important genes & mutations
Immunodeficient mice: Effects of Background strain on phenotypes
Immunodeficient mice: Primary T cell deficient options
Immunodeficient mice: MORE nude options
Immunodeficient mice: MORE scid options
Immunodeficient mice: MORE options
Page 5
Table II
Table IV
Table V
Table VI
Table VIa
Table VIb
Table VIc
Infectious Agents by site/system
Viruses – Test methods, prevalence
Bacteria – Test methods, prevalence
Eukaryotes – Test methods, prevalence
Protozoa by site
Fur mite comparison
Pinworm comparison
Page 9
WEB Resources
References
Page 14
TEXT SECTIONS see also [1]
Common disease conditions – Spontaneous or Non infectious
Common diseases – Infectious by system
Infectious agents, diagnostic methods
Infectious agents, Viruses
1. Comments by agent (alphabetical order)
V
Infectious agents, Bacteria
1. Comments by agent (alphabetical order)
VI
Infectious agents, Eukaryotes
1. Fungi – inc Protista – Comments by agent (alphabetical order)
2. Protozoa – Comments by agent (alphabetical order)
Metazoa - Comments by agent (alphabetical order)
a) Arthropods Comments by agent (alphabetical order)
b) Helminths Comments by agent (alphabetical order)
I
II
III
IV
cbrayton@jhmi.edu 2015
Page 1 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Table Ia: Non infectious CLINICAL conditions in mice at different ages [2] [3] [4]
Usually young mice
Life threatening
Hydrocephalus
Malocclusion
Usually not life
threatening
Microphthalmia
Usually adult,
various ages
Dermatitis and wounds
Abdominal enlargement (e.g.
hydrometra,
hydronephrosis, urinary
obstruction, ascites).
‘Neurologic’ signs (e.g. paresisparalysis, seizures, vestibular
signs)
Alopecia due to barbering
Blind / deaf
Usually older mice
Neoplasia (tumors)
Wasting due to chronic
progressive
conditions (e.g.
amyloidosis,
nephropathy,
neoplasia)
Obesity
Table Ib. Non infectious conditions and neoplasms in common mouse strains [2] [3] [4] [5]
129 strains
A/J
AKR
BALB/c
BALB/cBy
C3H
C57BL/6
DBA
FVB/N
Acallosity-hypocallosity; acidophilic macrophage pneumonia, hyalinosis
Lung tumors (testicular teratomas)
Congenital anomalies; amyloidosis; muscular dystrophy
Lung tumors, rhabdomyosarcoma [6]
Thymic lymphoma
Acallosity-hypocallosity; conspecific (male) aggression; cardiac calcinosis; cardiac thrombi;
vaginal septa
Lung tumors; Harderian gland tumors; myoepithelioma; rhabdomyosarcoma,
induced plasmacytoma
Blind (rd1/rd1); cardiac calcinosis, soft tissue mineralization
Mammary tumors in females; liver tumors in males
Hydrocephalus; microphthalmia; ulcerative dermatitis; amyloidosis; acidophilic macrophage
pneumonia, hyalinosis
Lymphoma; histiocytic sarcoma
dba, Cardiac calcinosis, soft tissue mineralization; seizures; deafness; glaucoma
BLIND rd1
Blind (rd1/rd1); seizures; mammary hyperplasia
Lung tumors
Diabetes; immune alterations
Lymphoma
Blind (rd1/rd1); conspecific (male) aggression; muscular dystrophy
Radioresistant
Lymphoma
Strain stock variations in: blind due to rd1/rd1; acidophilic macrophage pneumonia;
amyloidosis; nephropathy; urinary syndrome
Lymphomas; lung tumors; liver tumors; skin tumors
C3H, CBA, FVB, SJL, SWR etc
DEAF
C57BL/6, BALB, DBA, etc
NOD
SJL/J
Swiss
Not inbred
Re tumor frequency – see also http://tumor.informatics.jax.org/mtbwi/index.do
cbrayton@jhmi.edu 2015
Page 2 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Table Ic: Some immune relevant genotype variations (in common strains) [7]
Gene
symbol
Gene /Locus
name
Chro
m
Allele
Symbol
Allele name
H2
Major
Histocompatibility
Complex (MHC) H2
haplotypes
17
a
H2a *
H2
MHC H2 haplotype
17
b
H2b
H2
MHC H2 haplotype
17
b2
H2b2
H2
MHC H2 haplotype
17
d
H2d
H2
MHC H2 haplotype
17
g7
H2g7
H2
MHC H2 haplotype
17
k
H2k
H2
MHC H2 haplotype
17
P
H2p
C57BL/6, C57BL/10
129 - all?
NZB
DBA/2
BALB/c, BALB/cBy,
NOD
AKR,
C3H, CBA, MRL
P
H2
MHC H2 haplotype
17
q
H2q
DBA/1, FVB/N, Nu/J
H2
MHC H2 haplotype
17
r
H2r
RIII
H2
MHC H2 haplotype
17
s, s2
H2s,s2
SJL, SJL/J
H2
MHC H2 haplotype
17
z
H2z
NZW, NZN, NZO
b-1
b-1 responsive
b-2
b-2responsive
b-3
b-3 responsive
d
d non responsive
2
0
(Hc0)
0 = deficient
2 base "TA"
deletion
C57BL/6, C57, C58
BALB/c, BALB/cBy,
C3H/HeJ, CBA,
A/He, A/J
MOLF/Ei, M spretus,
M Caroli
129, 129P3J, AKR,
DBA/2, SJL/J
A/HeJ, AKR/J,
DBA/2J,
FVB/N; NOD/ShiLtJ;
NZB/B1NJ, SWR/J,
B10.D2/SnJ
7
Hc2-r
(Ceacam1
Hv2-r)
r = resistance to Mouse
hepatitis virus (MHV4)
Deletion 
23 aa
substitution
recessive
SJL/J
Inhibit killing activity
[8]
Various
B6, NOD, 129,
BALB/c
Inhibit killing activity
[8]
Various
B6, 129, BALB/c
Inhibit killing activity
[8]
Various
B6, NOD, BALB/c
Inhibit killing activity
[8] See NKC Natural
killer cell complex
Various
Various
Ahrb-1
Ahrb-2
Ahrb3
Aryl hydrocarbon
receptor
Ceacam1
Klra1
(Ly49a)
Klra2
Hemolytic
complement (c5)
carcinoembryonic
antigen-related cell
adhesion molecule
1
killer cell lectinlike receptor,
subfamily A1 (aka
Ly49A)
Klra1
6
C57BL/6
etc
Aka Ly49B
(formerly Cmv)
6
Aka Ly49C
6
Klra2
C57BL/6
etc
Klra3
Klra3
Klra5,6,9,
15,17,19,
20,21,22
*
Aka Ly49E,F,I,O,
Q,S,T,V
C57BL/6
etc
6
Strains
A (A/HeJ, A/J,
AWySnJ)
12
Ahrd
Hc
Mutation
See also http://jaxmice.jax.org/literature/catalog/mhc_h2_haplotypes.pdf
http://www.imgt.org/IMGTrepertoireMHC/Polymorphism/haplotypes/mouse/MHC/Mu_haplotypes.html
cbrayton@jhmi.edu 2015
Page 3 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Gene
symbol
Gene /Locus
name
Chro
m
Klra4,8,
13,14,16,
18,21,23,
25,28,30
Aka Ly4D,H,M,
N,P,R,U,W
6
Mx1
Mx1
myxovirus
(influenza virus)
resistance 1
NOD-like receptor
(NLR) 1a
Strains
Activate NK etc [8]
See NKC Natural killer
cell complex
Various
Various
Mx1 r
r = resistance to
Myxovirus (etc
intranuclear virus ) [9]
Wild type
A2G, SL/NiA, CAST/Ei
Mx1 s1
s1 = susceptibility 1
[10]
deletion
Nlrp1aR/R
11
Nlrp1as/s
Slc11a1
(Nramp1)
Slc11a1 r
solute carrier
family 11
member 1
1
Slc11a1
(Nramp1)
Slc11a1 s
T cell receptor
beta, variable 8
6
Tcrb-V8
Tlr4
Mutation
Mx1 s2
Nlrp1a
Tcrb-V8
Allele name
16
Mx1
Nlrp1a
Allele
Symbol
Toll like receptor 4
4
s2 = susceptibility 2
[10]
Resistance to anthrax
LT lethal toxin [11]
NLRs = inflammasome
components
Point
mutation
Sensitivity to anthrax
LT lethal toxin [11]
r = resistance to certain
agents – some
Mycobacterium sp,
S. typhimurium,
L donovani [12]
s = susceptibility to
certain agents – some
Mycobacterium sp,
S. typhimurium,
L donovani [12]
dominant
allele
(wild type?)
A; C3H; CBA; DBA
C3H/HeJ, 129/Sv
G to A
point
mutation
recessive
BALB/c; C57BL
Tcrb-V8 a
a = wild type
Wild type
Tcrb-V8 c
c
d = defective
lipopolysaccharide
response
deletion
C to A
substitution
in 3rd exon
Lps-d
(Tlr4Lps-)d
Most common strains
C57BL/6J, C3H/HeJ,
BALB/cJ,etc
CBA/J, CE/J, I/LnJ,
PERA/Ei
C57BL/6J, A/J, I/LnJ,
SPRET/EiJ, PWK/PhJ,
PWD/PhJ, AKR/J,
NOD/LtJ, DBA/2J
129S1/SvlmJ, Balb/cJ,
FVB/NJ, SWR/J,
CAST/EiJ, C57/LJ
A/J, AKR/J, BALB/cJ,
C3H/HeJ, C57BL/10J,
C57BL/6J, MRL
C57L/J, SJL/J
C3H/HeJ
Search for gene/locus/allele updates at http://www.informatics.jax.org/
Search for mice at http://www.findmice.org/index.jsp
cbrayton@jhmi.edu 2015
Page 4 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Table Id: Immunodeficient mice: properties of important genes & mutations
Gene
B2m
beta-2
microglobulin
PROPERTIES
Required for normal expression of major histocompatibility class I proteins (displaying viral and self
antigens to potentially responsive T cells) and for
CD8+ T cell maturation and NK cell development
Tm Deficiency   NK cell development/activity, CD8+ T cell activity
Foxn1
forkhead box N1,
formerly Hfh11
nu mutation known as nude.
nu/nu are ‘hypothymic’ – ‘athymic’  T cell deficient; respond poorly to thymus-dependent antigens,
should accept allogeneic and xenogeneic grafts(but may have NK activity & be leaky)
 Greatly increased susceptibility to infection.
Il2rg
interleukin 2
receptor, gamma
chain
Necessary for IL2, IL4, IL7, IL9, IL15, IL21 high affinity binding & signaling.
Role in mediating susceptibility to thymic lymphomas in mice
Myd88
myeloid
differentiation
primary response
gene 88
Tm Deficiency  NO NK development +other defects in innate immunity.
 NO thymic lymphomas on susceptible background
Myd88 is an adapter protein used by all TLRs (except TLR 3) to activate transcription factor NF-κB in
innate immunity
Tm Deficiency   innate responses – neutrophils, macrophages,
hematopoietic, molecular signaling, and apoptotic abnormalities
Prf1
perforin 1
(pore-forming
protein)
Essential in lytic pathway by which NK and CD8+ lymphocytes kill targeted cells.
Prkdc
protein kinase,
DNA-activated,
catalytic
polypeptide
Involved in repairing double-stranded DNA breaks and in recombining the variable (V), diversity (D), and
joining (J) segments of immunoglobulin and T-cell receptor genes.
scid mutation/allele ‘severe combined immunodeficient’
Rag1
recombination
activating gene 1
Ticam1
Trif
Tm Deficiency   NK cell activity, CD8+ T cell activity
scid/scid  no mature T and B cells, cannot mount cell mediated or humoral adaptive immune
responses, do not reject allogeneic and xenogeneic grafts,  useful cancer research models.
BUT leakiness  some functional B and T cells as they age.
 leakiness in non-SPF conditions,
 leakiness C57BL/6J, BALB/cByJ backgrounds > C3H/HeSnJSmn > NOD/LtSzJ background
++ radiosensitive  cannot be as thoroughly irradiated as other immunodeficient models before
being engrafted
renders NOD mice diabetes-free  useful for adoptive transfer of diabetes by T cells.
Essential for V(D)J gene rearrangements necessary  functional antigen receptors in T & B cells.
Deficient tm1Mom mutants  no mature, functional T and B cells.
Renders NOD mice diabetes-free.
+ B cell lymphomas in NOD.129S7(B6)-Rag1tm1Mom/J
Toll-like receptor adaptor molecule 1 (formerly uses TIR-domain–containing adapter-inducing interferon-b
(TRIF)). adapter protein used by TLR 3 to activate transcription factor NF-κB in innate immunity
Tm Deficiency   innate responses – esp when combined with Myd88 Tm
Tm = targeted mutation (knockout)
cbrayton@jhmi.edu 2015
Page 5 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Table Ie: Immunodeficient mice: Effects of Background strain on phenotypes
adapted from Jaxnotes #501, 2006 [13] http://jaxmice.jax.org/jaxnotes/archive/501.pdf etc
Back ground
Innate
NK etc
Leaky
H2
Examples
‘common name’
Characteristics (Phenotypes)
BALB
Substrains
Normal
Yes T
d
CByJ.Cg-Foxn1nu/J
‘BALB/c Nude’
Normal B cells etc
 Extra thymic T cells with age
POOR breeders,  female fertility  nu/+ F x nu/nu M
Normal
High
d
CBySmn.CB17Prkdcscid/J
‘BALB/c scid’
NO functional B and T cells
HIGH NK, complement activity (normal APC function)
Thymic lymphomas but < NOD.CB17-Prkdcscid/SzJ
RadioSensitive
Normal

NO
b
B6.129S7Rag1tm1Mom/J
‘B6 Rag’
NO functional B and T cells
HIGH NK activity (normal APC, complement c5)
Normal

High
b
B6.CB17Prkdcscid/SzJ
‘B6 scid’
NO functional B and T cells
HIGH NK, complement activity (normal APC function)
Low
NO
g7
NOD/ShiLtJ
‘NOD’
susceptible to immune mediated insulitis + diabetes
 NK, macrophage, APC activity; Hc0  No c5
RadioResistant
Low
NO
g7
NOD.129S7(B6)Rag1tm1Mom/J
‘NOD Rag’
NO functional B and T cells
Pre-B cell > Thymic lymphomas ( 10.5mo)
Somewhat RadioResistant
Low
NO
g7
NOD.Cg-Rag1tm1Mom
Prf1tm1Sdz/SzJ
‘NOD Rag Prf’
NO functional B and T cells,
NO NK cell activity; Hc0  No c5
Thymic lymphomas (short lifespan ~8.5mo)
RadioResistant: survives up to 8 Gy
Low
Low
g7
NOD.CB17Prkdcscid/J
NO functional B and T cells
NOD background  low NK activity, Hc0  No c5,
defects in myeloid development, poor APC functions
Thymic lymphomas (short lifespan ~8.5mo)
RadioSensitive: tolerates up to 4 Gy
Low
Low
g7
NOD.Cg-Prkdcscid
B2mtm1Unc/J
‘NOD scid B2m’
NO functional B and T cells
No MHC 1 expression  ~NO NK activity
Thymic lymphomas (short lifespan ~6.3mo)
Hemochromatosis dt?
Low
NO
g7
NOD.Cg-Prkdcscid
Il2rgtm1Wjl/SzJ
‘NSG’
NO lymphocytes; NO NK cell activity
Lymphoma-resistant; long-lived > 16m
JAX
Low
NO
NOD.Cg-Prkdcscid
Il2rgtm1Sug/JicTac
‘NOG’
NO lymphoctes; NO NK cell activity
Lymphoma-resistant; long-lived > 16m
TAC
Normal
Yes T
NU/J
NIH outbred nude stock  inbred at TJL
C57BL/6J
NOD
NIH stock
cbrayton@jhmi.edu 2015
q
(Foxn1nu)
Page 6 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Table If: Immunodeficient mice: Primary T cell deficient options
T
Cells
B
Cells
Innate
NK
Leaky
Foxn1nu (nude)
D
N
N
yes
Prkdcscid (scid)
D
D
N-
yes
Thymic
lymphoma
PrkdcscidLystbg
(scid-bg)
D
D
N
Reduced by
bg
Thymic
lymphoma
NOD-Prkdcscid
(NOD-scid)
D
D
D
Minimal
Thymic
lymphoma
NOD, Prkdcscid,
Il2rgtm… (NSG, NOG)
D
D
D
No
Mutations
Tumors
D = Deficient, N = ‘normal’
Table Ig: Immunodeficient mice: MORE NUDE options
Source
Background
Name
INbred
AKA
Tac Bom
B6N/Tac
B6.Cg/NTac-Foxn1nu
+
B6 nude
BALB/cAnN
C.Cg/AnNTac-Foxn1nu
+
BALB/c nude
BALB/cBom
C.Cg/AnBomTac-Foxn1nu
+
BALB/cA nude
BALB x NIH(S)
CrTac:NCr-Foxn1nu
NIH Nude
NMRI (S)
BomTac:NMRI-Foxn1nu
Swiss nude
NIH(S)
NTac:NIHS-Foxn1nu
Swiss Nude
BALB/cAnN
CAnN.Cg-Foxn1nu/Crl
CD1 (S)
Crl:CD1-Foxn1nu
CD1 nude
NIH Nu
Crl:NU-Foxn1nu
Nu/nu NIH nude
NIH Nu
Hsd:Athymic Nude-Foxn1nu
NIH nude
NMRI (S)
HsdCpb:NMRI-Foxn1nu
Swiss Nude
CD1 (S)
HsdHli:CD1-Foxn1nu
CD1 nude
ICR (S)
HsdOla:ICRF-Foxn1nu
Swiss Nude
MF1
HsdOla:MF1-Foxn1nu
MF1 nude
BALB/cOla
BALB/OlaHsd-Foxn1nu
Crl
Hsd Ola
cbrayton@jhmi.edu 2015
+
+
BALB/c Nude
BALB nude
Page 7 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Table Ih: Immunodeficient mice: MORE scid options
Source
Background
Name
INbred
AKA
Tac Bom
C.B17
C.B-Igh-1b/IcrTac-Prkdcscid
+
CB17 scid
IcrTac
IcrTac:ICR-Prkdcscid
C.B-17
C.B-Igh-1b/GbmsTac-Prkdcscid-Lystbg
+
scid-beige
NOD +
NOD/MrkBomTac-Prkdcscid
+
NODscid
NOD
NOD.Cg-Prkdcscid Il2rgtm1Sug/JicTac
+
CIEA NOG
C.B17
CB17/Icr-Prkdcscid/IcrCrl
+
CB17 scid
Crl
Scid - Swiss
Crl:SHO-PrkdcscidHrhr
Hsd Ola
Scid hairless outbred
BALB/cJ
BALB/cJHan™Hsd-Prkdcscid
+
BALB/c Scid
C.B-17
C.B-17/IcrHsd-Prkdcscid
+
CB17 scid
C3H
C3H.C-Prkdcscid/IcrSmnHsd
+
C3H scid
ICR (S)
HsdIcr:Ha(ICR)-Prkdcscid
NOD CB17
NOD.CB17/JHliHsd-Prkdcscid
Scid
+
NODscid
Table Ii: Immunodeficient mice: MORE options
Source
Background
Name
INbred
AKA
Tac Bom
C.B17
C.B-Igh-1b/IcrTac-Prkdcscid
+
CB17 scid
IcrTac
IcrTac:ICR-Prkdcscid
C.B-17
C.B-Igh-1b/GbmsTac-Prkdcscid-Lystbg
+
scid-beige
NOD +
NOD/MrkBomTac-Prkdcscid
+
NODscid
NOD
NOD.Cg-Prkdcscid
+
CIEA NOG
C.B17
CB17/Icr-Prkdcscid/IcrCrl
+
CB17 scid
Crl
Il2rgtm1Sug/JicTac
Scid - Swiss
Crl:SHO-PrkdcscidHrhr
Hsd Ola
Scid hairless outbred
BALB/cJ
BALB/cJHan™Hsd-Prkdcscid
+
BALB/c Scid
C.B-17
C.B-17/IcrHsd-Prkdcscid
+
CB17 scid
C3H
C3H.C-Prkdcscid/IcrSmnHsd
+
C3H scid
ICR (S)
HsdIcr:Ha(ICR)-Prkdcscid
NOD CB17
NOD.CB17/JHliHsd-Prkdcscid
cbrayton@jhmi.edu 2015
Scid
+
NODscid
Page 8 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Table II Infectious agents in mice, listed by site or by primary disease phenotype when there are
pathology findings (2014 FELASA recommendations [14]
Viruses
ENTERIC-HEPATIC
Calicivirus (MNV)
Coronavirus (MHV)
Reovirus 3 (Reo3)
Rotavirus EDIM
Bacteria
ENTERIC-HEPATIC
Citrobacter rodentium
Escherichia coli
Clostridium piliforme
Cl difficile, Cl perfringens
Helicobacters
Salmonella enterica Ssp typhimurium
Segmented Filamentous Bacteria (SFB)
RESPIRATORY
Paramyxovirus (MPV,Sen)
RESPIRATORY
Bordetella avium, hinzii, etc,
CAR bacillus
Klebsiella pneumoniae
Mycoplasma pulmonis
Pasteurella pneumotropica
SKIN (or abscesses)
Corynebacterium bovis
Corynebacterium kutscheri
Staphylococcus aureus
Staphylococcus species
Streptobacillus moniliformis
IMMUNE or MULTISYSTEM
Adenovirus (MAV1,2)
Arterivirus (LDV)
Ectromelia virus (ECT)
Herpesvirus (MCMV, MTV)
LCM virus (LCMV)
LDH virus (LDV)
Papova (K, MPyV)
Parvoviruses (MMV, MPV)
CNS
PicoRNAvirus (TMEV etc)
OTHER
Hantavirus (HAN)
Retrovirus
Papillomavirus
cbrayton@jhmi.edu 2015
Bacteremia, Septicemia
Klebsiella oxytoca etc spp
Proteus mirabilis
Pseudomonas aeruginosa
Streptococcus, Enterococcus spp.
OTHER
Chlamydiae
Mycobacterium spp
Mycoplasmas Haemotrophic
Eukaryotes
ENTERIC-HEPATIC
Fungi
Gastric yeasts Kazachstania
Protozoa
Eimeria sp.
Cryptosporidia (C muris, C parvum)
Entamoebae
Flagellates
Nematodes
Aspiculuris tetraptera
Syphacia muris
Syphacia obvelata
Cestodes
Cysticercus fasciolaris
Hymenolepis diminuta
Rodentolepis microstoma
Rodentolepis nana
RESPIRATORY
Fungi
Pneumocystis murina
SKIN/HAIR
Fungi
Dermatophytes
Arthropods
Lice
Mites – Fur, Follicles
OTHER
Fungi
Encephalitozoon cuniculi
Protozoa
Klossiella muris
Sarcocystis muris
Arthropods
Mesostigmatid mites
NON parasitic arthropods Psocids
Page 9 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Table IV Viruses in mice: test methods and results from Pritchett-Corning, Cosentino, Clifford (2009) [15]
(see also [16] [17] [18] [19]), and survey results from Carty 2008. [20] The most common agents are in
boldface. The final column indicates if PCR testing is commercially available on feces (F) or biological
materials (B) such as serum or cultured cells.
Agent (common abbreviations)
Primary
test [15]
Confirmatory test
%Pos
Results
[15]
[15]
Adenoviridae
MFIA/
IFA
Mouse Adenovirus (MAV1, MAV2)
ELISA
Arenaviridae
MFIA/
Lymphocytic choriomeningitis virus
IFA
ELISA
(LCMV)
Arteriviridae
Lactate dehydrogenase-elevating virus
Enzyme*
PCR
(LDHV, LDV)
Caliciviridae
MFIA/
IFA
Murine norovirus (MNV)
ELISA
Coronaviridae
MFIA/
IFA
Mouse Hepatitis virus (MHV)
ELISA
Bunyaviridae
MFIA/
IFA
Hantavirus (HAN, HNT)
ELISA
Herpesviridae
MFIA/
IFA
Mouse Cytomegalovirus (MCMV)
ELISA
Herpesviridae
IFA
PCR
Mouse Thymic virus (MTV, MTLV)
Paramyxoviridae
MFIA/
IFA, HAI
Pneumonia virus of mice (PVM)
ELISA
Paramyxoviridae
MFIA/
IFA, HAI
Sendai virus (SEND, SEN)
ELISA
Parvoviridae
MFIA/
IFA, HAI
Parvovirus (MVM, MMV)
ELISA
Parvoviridae
MFIA/
IFA
Parvovirus (MPV 1,2)
ELISA
Picornaviridae (Cardiovirus)
MFIA/
Theiler's murine encephalomyelitis virus
IFA
ELISA
(TMEV, GD-VII)
Polyomaviridae
MFIA/
IFA
Mouse Pneumonitis virus (K)
ELISA
Polyomaviridae
MFIA/
IFA
Polyoma virus (POLY)
ELISA
Poxviridae (Othopoxvirus)
MFIA/
IFA
Ectromelia virus (ECTRO, ECT))
ELISA
Reoviridae
MFIA/
IFA, HAI
Reovirus (REO, REO3)
ELISA
Reoviridae
MFIA/
IFA
Mouse Rotavirus (EDIM, MRV)
ELISA
Abbreviations in the table – see also section diagnostic methods
%Pos
inst. [20]
Comment
0.02
FB
0.01
Zoonotic
B
Chem
B
32.3
1.6
PCR?
FB
>40%
0
FB
Zoonotic
0.04
B
B
B
0.01
B
0
B
0.3
~40%
FB
1.8
>90%
FB
0.26
FB
0
B
0.02
B
0.02
B
0.01
B
0.56
~30%
FB
Chem – plasma chemistry test for elevated lactate dehydrogenase activity
ELISA - Enzyme-linked immunosorbent assay
IFA - Indirect immunofluorescence assay
MFIA - multiplexed fluorometric immunoassay
HAI - Hemagglutination inhibition assay
PCR - Polymerase Chain reaction
cbrayton@jhmi.edu 2015
Page 10 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Table V Bacteria in mice: Test methods and results (expressed as % positive results) from PritchettCorning, Cosentino, Clifford (2009),[15] and survey results from Carty 2008.[20] Agents in boldface were
detected in > 1% of specimens. Gram positive bacterial agents are shaded. The final column indicates if
PCR testing is commercially available for specimens from feces, oral or lung swab, or skin/fur swab.
% POS
Results[15]
% Pos
institution[20]
Agent
Method[15]
PCR?
Bordetella bronchiseptica
Culture
0.00
Feces
Cilia-associated respiratory bacillus
Serology
0.01
Oral/lung
Citrobacter rodentium
Culture
0.00
Feces
Culture
2
PCR
9
Skin swab
Feces
Corynebacterium bovis
Corynebacterium kutscheri
Culture
0.00
Helicobacter genus (any sp.)*
PCR
16.08
- Helicobacter hepaticus
PCR
12.37
Feces
- Helicobacter bilis
PCR
2.17
Feces
Klebsiella oxytoca
Culture
0.38
> 40%
Feces
Klebsiella pneumoniae
Culture
0.10
>30%
Feces
Culture
0.00
Serology
0.01
PCR
0.00
Pasteurella multocida
Culture
0.00
Pasteurella pneumotropica
Culture
12.90
Other Pasteurella species
Culture
0.31
Any Salmonella species
Culture
0.00
Feces
Staphylococcus aureus
Culture
6.07
Feces
Streptobacillus moniliformis
Culture
0.00
Feces
Streptococcus pneumoniae
Culture
0.00
Feces
Streptococcus sp. – β-haemolytic, Group B
Culture
0.24
Feces
Streptococcus sp. – β-haemolytic, Group G
Culture
0.00
Feces
Mycoplasma pulmonis
cbrayton@jhmi.edu 2015
~80%
Feces
<10%
Feces
>70%
Feces
Page 11 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Table VI: Eukaryotes: Test methods and results from Pritchett-Corning, Cosentino, Clifford (2009),[15]
and survey results from Carty 2008. [20] The most common agents are in boldface. The final column
indicates if PCR testing is available for specimens from feces, oral or lung swab, or skin/fur swab.
Agent
Fungi
Encephalitozoon cuniculi
Pneumocystis spp (submissions from
immunodeficient mice)
Enteric Protozoa
Chilomastix sp.
Entamoeba sp.
Giardia sp.
Hexamastix sp.
Monocercomonoides sp.
Retortamonas sp.
Spironucleus sp.
Trichomonads
Metazoa – enteric
Oxyurids (pinworms)
Aspiculuris tetraptera
Syphacia muris
Syphacia obvelata
Metazoa – Surface, external
Lice
Fur Mites
Method[15]
%Pos
Results [15]
Serology
0.00
PCR
1
%Pos
institutions[20]
PCR?
Oral/ lung
Not evaluated
Wet mount
Wet mount
Wet mount
Wet mount
Wet mount
Wet mount
Wet mount
Wet mount
3.74
8.08
0.00
4.45
0.04
0.03
0.08
8.88
Feces
Feces
>70%
Direct
Direct
Direct
0.19
0.25
0.11
Direct
Direct
0.00
0.12
Feces
Feces
Feces
~40%
Fur swab
Table VIa protozoa in mice by type and by site of infection
Flagellates
Coccidia
Other
Stomach
Cryptosporidium muris
Duodenum
Giardia sp.
Spironucleus sp.
Large Intestine
Chilomastix sp.
Hexamastix sp.
Monocercomonoides sp.
Retortamonas sp.
Trichomonads
T muris, T diminuta etc
cbrayton@jhmi.edu 2015
Jejunum
Eimeria spp.
Ileum
Cryptosporidium parvum
Large Intestine
Entamoeba muris
Kidney
Klossiella muris
Muscle
Sarcocystis muris
Various tissues
Toxoplasma gondii
Kidney / Brain
[E cuniculi – now a fungus]
Page 12 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
Table VIb Features of common fur mites (oversimplified) [21]
Elongate body with
bulges between
the legs
1st pair of legs (tarsi)
are specialized for
hair clasping;
2nd pair of legs has
single empodial
claw
300-500u long
Elongate body with
bulges between the
legs
1st pair of legs (tarsi)
are specialized for
hair clasping;
2nd pair of legs has 2
unequal claws
Rounded-oval
T romboutsi
(formerly
M romboutsi)
Rounded-oval
3rd and 4th pairs of
legs (tarsi) are
short thick and
specialized for hair
clasping
3rd and 4th pairs of
legs (tarsi) are
short thick and
specialized for hair
clasping
300-500u long
Oval up to 250u long
Base of hair shaft
Feeds on interstitial
fluids
Deep among hairs
especially of dorsal
head neck
shoulders flank
Probably similar to
M musculi
Probably similar to
M musculi
Females oval 130x
350u
Males rounded <
200u diameter
Oval up to 450u long
Distal hair shaft
Feeds on superficial
keratin layer
Among hairs
especially of
inguinal skin and
ventrum
Females oval < 300u
long
Males rounded <
200u diameter
Probably similar to
M musculinus
Probably similar to
M musculinus
R affinis
(formerly M affinis)
M. musculi
Body shape
Distinguishing
features
Adult size
Eggs
Diet
Site on mouse
M. musculinus
TABLE VIc Comparison of features of Syphacia obvelata and Aspiculuris tetraptera [21] [22] [23]
Prepatent period
(egg to egg)
Infectious form
Larvae hatch from eggs
Adult Size
Aspiculuris tetraptera
21-25 days
Syphacia obvelata
11–15 days
S muris
7-8 days
Embryonated eggs
Cecum
2-4mm long,
< 200u wide
Embryonated eggs
Cecum
1-6mm long,
(males are shorter)
< 400u wide
Cecum lumen; females
transit colon to
deposit eggs at anus
350 per female all at
once
Not embryonated;
On perineum;
Embryonate within 24hr
~36u wide`, 134u long;
crescentic, flattened
on 1 side, pointed
ends
~ S obvelata
~ S obvelata
1-4mm long,
(males are shorter)
< 200u wide
~ S obvelata but eggs
deposited after
noon
all at once in
afternoon
Not embryonated;
On perineum;
Embryonate within
few hours
~30u wide, 75u long;
flattened on 1 side
Adult location
Colon lumen
# eggs
17 per female per day,
intermittently
Not embryonated
Shed in feces;
Embryonate in 5-8days
~40u wide, 90u long;
symmetric,
Unembryonated in fresh
feces
Eggs
cbrayton@jhmi.edu 2015
Page 13 of 14
Mouse Diseases Common Conditions (phenotypes) and Infectious Diseases TABLES only
WEB RESOURCES
1. Frith, and Ward, J. M. A Color Atlas of Neoplastic and Non Neoplastic Lesions in Aging Mice. 1988.
http://www.informatics.jax.org/frithbook/ also print on Demand from http://store.cldavis.org/
2. IMSR - FIND MICE AND ES CELL LINES http://www.findmice.org/index.jsp
3. Mouse (rat) and Gene NOMENCLATURE & apply for a laboratory code http://www.findmice.org/index.jsp
4. Mouse Phenome database - mouse data and protocols http://phenome.jax.org/
5. Mouse Tumor Biology (MTB) Database & tumor frequency grid
http://www.informatics.jax.org/mtbwi/index.do
6. NTP NON neoplastic lesion atlas http://ntp.niehs.nih.gov/nnl/
7. Pathbase: European mutant mouse pathology database http://www.pathbase.net/
8. RENI Tissue trimming guide http://reni.item.fraunhofer.de/reni/trimming/index.php
REFERENCES
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
Danneman, P., Suckow, M., and Brayton, C. THE LABORATORY MOUSE. 2nd ed. 2012. Boca Raton, FL: Taylor and
Francis, CRC Press.
Brayton, C.F., Treuting, P.M., and Ward, J.M. Pathobiology of aging mice and GEM: background strains and
experimental design. Vet Pathol. 2012. 49(1):85-105.
Brayton, C. and Treuting, P. Phenotyping, in Comparative Anatomy and Histology: A Mouse and Human Atlas Treuting,
P. and Dintzis, S., Eds. 2012. Elsevier (Academic Press): London. pp. 361-381.
Brayton, C. Nature and Nurture: impacts on mouse phenotypes and translational research, in THE MOUSE AS A
MODEL ORGANISM, Pihlajaniemi, T. and Brakebusch, C., Eds. 2011. Springer.
Festing, M.F.W. Inbred strains: Index of Major Mouse Strains. 1998 April 9, 1998 July 1, 2013]; Available from:
http://www.informatics.jax.org/external/festing/mouse/STRAINS.shtml.
Sher, R.B., Cox, G.A., Mills, K.D., and Sundberg, J.P. Rhabdomyosarcomas in aging a/j mice. PLoS One. 2011.
6(8):e23498.
Sellers, R. The Gene or not the Gene: That is the Question Understanding the Genetically Engineered Mouse Phenotype
Vet Pathol. 2012 49(1):in Press
Schenkel, A.R., Kingry, L.C., and Slayden, R.A. The ly49 gene family. A brief guide to the nomenclature, genetics, and
role in intracellular infection. Front Immunol. 2013. 4:90.
Vanlaere, I., Vanderrijst, A., Guenet, J.L., De Filette, M., et al. Mx1 causes resistance against influenza A viruses in the
Mus spretus-derived inbred mouse strain SPRET/Ei. Cytokine. 2008. 42(1):62-70.
Staeheli, P., Grob, R., Meier, E., Sutcliffe, J.G., et al. Influenza virus-susceptible mice carry Mx genes with a large
deletion or a nonsense mutation. Mol Cell Biol. 1988. 8(10):4518-23.
Sastalla, I., Crown, D., Masters, S.L., McKenzie, A., et al. Transcriptional analysis of the three Nlrp1 paralogs in mice.
BMC Genomics. 2013. 14:188.
Wessling-Resnick, M. Nramp1 and Other Transporters Involved In Metal Withholding During Infection. J Biol Chem.
2015.
JAX. Choosing an Immunodeficient Mouse Model. JAXNOTES. 2006.
Felasa working group on revision of guidelines for health monitoring of rodents and rabbits. FELASA
recommendations for the health monitoring of mouse, rat, hamster, guinea pig and rabbit colonies in breeding and
experimental units. Lab Anim. 2014. 48(3):178-192.
Pritchett-Corning, K.R., Cosentino, J., and Clifford, C.B. Contemporary prevalence of infectious agents in laboratory
mice and rats. Lab Anim. 2009. 43(2):165-173.
Mahler, M. and Kohl, W. A serological survey to evaluate contemporary prevalence of viral agents and Mycoplasma
pulmonis in laboratory mice and rats in western Europe. Lab Anim (NY). 2009. 38(5):161-5.
Liang, C.T., Shih, A., Chang, Y.H., Liu, C.W., et al. Microbial contaminations of laboratory mice and rats in taiwan from
2004 to 2007. J Am Assoc Lab Anim Sci. 2009. 48(4):381-6.
Khan, I.H., Kendall, L.V., Ziman, M., Wong, S., et al. Simultaneous serodetection of 10 highly prevalent mouse
infectious pathogens in a single reaction by multiplex analysis. Clin Diagn Lab Immunol. 2005. 12(4):513-9.
Baker, D.G. Natural Pathogens of Laboratory Mice, Rats, and Rabbits and Their Effects on Research. Clin Microbiol
Rev. 1998. 11(2):231-266.
Carty, A.J. Opportunistic infections of mice and rats: Jacoby and Lindsey revisited. ILAR J. 2008. 49(3):272-6.
Baker, D.G. Flynn’s Parasites of Laboratory Animals. 2nd ed. ACLAM, Blackwell Publishing.ed, Ed. Baker, D.G. 2007:
ACLAM, Blackwell Publishing.
Taffs, L.F. Pinworm infections in laboratory rodents: a review. Lab Anim. 1976. 10(1):1-13.
Clifford, C.B. and Watson, J. Old enemies, still with us after all these years. ILAR journal / National Research Council,
Institute of Laboratory Animal Resources. 2008. 49(3):291-302.
cbrayton@jhmi.edu 2015
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