EPAPS Document No.: To be filled in by AIP staff]: [to be filled in by AIP staff]
All Authors: Surendra K. Yadav, Jay Singh, Ved V. Agrawal and B. D. Malhotra
Title: Nanostructured nickel oxide film for application to fish freshness biosensor
Description: Fig [S1] Scheme
Fig [S2] Hall–Williamson plot
Fig [S3] FTIR
Fig [S4] SEM
Fig [S5] Contac angle measurement
Fig [S6] EIS
Fig [S7] Scan rate
Fig [S8] Response time
Fig [S9] Shelf-life
Fig [S10] Real sample analysis
Table I
Total No. of Files: 1
File Names: README.TXT, and supplementary file
File Types: Doc
Special Instructions: None
Contact Information: Prof. B.D. Malhotra,
Department of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Delhi
110042, India.
Email: bansi.malhotra@gmail.com, Tel.: +91-11-27294668
Fax: 91-11-27871023
Supplementary File
Fig [S1] Schematic representation of xanthine biosensor and mechanism of electron
exchange between xanthine oxidase and electrode surface.
cos
Fig [S2] Hall–Williamson plot of the n-NiO nanoparticles.
2.8x10
-3
2.6x10
-3
2.4x10
-3
2.2x10
-3
2.0x10
-3
1.8x10
-3
1.6x10
-3
1.4x10
-3
1.2x10
-3
Slope of the Curve = - 3.46x10
-3
0.60 0.65 0.70 0.75 0.80 0.85 0.90 0.95 1.00
Sin
Fig [S3] Fourier Transform Infrared spectra of (a) n-NiO/ITO electrode and (b) XOx/nNiO/ITO bioelectrode.
400
3462
1630
682 462
Transmittance(a.u.)
350
300
2581
2359
1700
2208
1377
2230
1830
250
200
150
682
780
3090
b
a
3746
100
940
1169
50
4000
3500
3000
2500
2000
-1
Wavenumber (cm )
1500
1000
500
Fig [S4] Scanning electron microscopic images of n-NiO nanoparticle (a & b) and XOx/nNiO/ITO (c & d) bioelectrode at different magnification.
(a)
(b)
(c)
(d)
Fig [S5] Contact Angle measurement of ITO electrode (i), n-NiO nanoparticle (ii) and
XOx/n-NiO/ITO bioelectrodes (iii)
CA 1200
(i)
CA 980
(ii)
CA 680
(iii)
Fig [S6] The Nyquist plot of the electrochemical impedance spectra of (a) ITO, (b) nNiO/ITO electrode (c) XOx/n-NiO/ITO bioelectrode having phosphate buffer (50mM, pH
7.0, 0.9% NaCl) containing 5mM [Fe(CN)6]3−/4−.
Fig [S7] Electrochemical response curve potential V/S current as a function of scan rate inset
current vs (scan rate)1/2.
Fig [S8] Electro chemical response time on XOx/n-NiO/ITO bioelectrode from 5 to 40
seconds of incubation period.
-5
8.9x10
-5
8.8x10
-5
8.7x10
Current (A)
-5
8.6x10
-5
8.5x10
-5
8.4x10
-5
8.3x10
-5
8.2x10
-5
8.1x10
-5
8.0x10
0
5
10
15
20
25
30
Response Time (s)
35
40
45
Fig [S9] Self life curve for XOx/n-NiO/ITO bioelectrode as a function of time.
Current (A)
Fig [S10] Linearity curve for real sample (fish) analysis for xanthine estimation.
2.0x10
-4
1.5x10
-4
1.0x10
-4
5.0x10
-5
0.0
-5.0x10
-5
-1.0x10
-4
-1.5x10
-4
-2.0x10
-4
1
2
3
4
Days
5
6
7
Table I. Characteristics of the xanthine oxidase based biosensor including those reported in
literature.
Material
Detection
Linearity
Sensitivity
Response
Storage
(%)
limit (μM)
(μM)
(µA/µM-cm2)
time(s)
Time(days)
Loss
Ref.
Graphite
1.5
1.5-70
0.39
60
------
------
1
Graphite rod
0.1
0.1-0.6
------
35
15
------
2
Polypyrrole
1
1-20
------
------
------
------
3
Zinc oxide-PPY
0.8
0.8–40
------
5
100
40
4
Nafion
0.5
2-185
------
30
10
------
5
2
2–50
------
150
--------
------
6
Au-polypyrrole
0.4
0.4 - 100
------
------
100
40
7
Modified graphite
4.5
0-40
0.210
120
500 h
35
8
(c-MWCNT)(PANI)
0.6
0.6–58
------
------
100
50
9
------
1 - 100
------
150
------
------
10
CNT glassy carbon
0.1
0.2 - 10
------
------
15
-----
11
Glassy carbon paste
5.3
20-80
------
------
------
------
12
ZnO/Ch/MWCNT/PANI
0.1
0.1–100
------
4
30
30
13
n-NiO Nanoparticle
2.9
10-200
0.6214
15
100
30
Present
DW-CNT
MWCNT
Work.
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