Pronuclear Injection
Price
UO Investigators
Non-UO Investigators
Standard plasmid injection in B6D2F1
$1750
$3800
Standard plasmid injection in C57Bl/6J
$1950
$4000
BAC injection
$2150
$4200
Test injection (one day only, no guaranties)
$750
$1500
Plasmid DNA digestion and purification
$300
$500
DNA isolation and PCR genotyping
$5/sample
$8/sample
Optional Services
Service Request
1. Fill out a service request form and send to tmf@uoneuro.uoregon.edu
2. Please send a copy of your IACUC approved protocol and your IBC
(Institutional Biosafety Committee) registration information
3. Submit a map of the construct, the method of extraction and a picture of the
purified fragment
4. Provide three 25 l aliquots at 0.5ng/l and 1 ng/l concentration. Please
label the tubes clearly and legibly.
Service Description
1.
2.
3.
4.
5.
6.
7.
8.
purchase egg donors and recipient females (week 1)
superovulated donors and mate with stud males (week 2)
plug check for pseudopregnant foster mothers mated to vasectomized males
egg harvest
injection of construct into pronucleus of zygotes
overian transfer of successfully injected eggs (week 3)
weaning, ear tagging and tail biopsy of resulting offspring (week 8)
transfer of tail biopsies and transgenic founders to the client (week 9)
Service Guaranties with Standard Injection
1. A minimum of 300 eggs will be injected.
2. Production of a minimum of 30 offspring
Blastocyst Injection
Price
UO Investigators
Non-UO Investigators
Blastocyst injection into C57Bl/6J
$2500
$4750
Blastocyst injection into Albino-B6
$2700
$4950
Breeding of Chimeras (two litters max.)
$900
$1100
Pathogen testing (out-side clones)
$200
$200
Service Request
1. Fill out a service request form and send to tmf@uoneuro.uoregon.edu
2. Please send a copy of your IACUC approved protocol and your IBC
registration information
Service Description
1.
2.
3.
4.
5.
6.
7.
8.
9.
Expansion of 2 ES cell clones on MEF feeders
purchase of egg donors and recipient females
superovulated donors and mate with stud males
plug check for pseudopregnant foster mothers mated to vasectomized males
morula or blastocyst harvest
ES cell injection into blastocoel
Overian or uterian transfer of successfully injected blastocysts
Weaning and ear tagging of resulting offspring
transfer of chimeras to the client
Service Guaranties
Injection of 2 independent ES cell clones per project into a minimum of 30
blastocysts each
Rederivation/ Cryopreservation/ IVF
Price
UO Investigators
Non-UO Investigators
Rederivation on C57Bl/6J
$375
$450
Rederivation on ICR
$325
$400
Rederivation (females provided by PI)
$300
$375
Embryo Cryopreservation
$950
$1250
Sperm Cryopreservation
$300
$500
One time shot cryo
$600
$850
IVF/Rapid colony expansion
$1500
$2000
Resurrection of frozen embryos
$275
$350
Service Request
1. Fill out a service request form and send to tmf@uoneuro.uoregon.edu
2. Please send a copy of your IACUC approved protocol and your IBC
registration information
3. Rederivations: two proven breeder males age 2-8 month, four 3-4 week old
females if provided by PI
4. Cryopreservation: five proven breeder males age 2-8 month, 15-20 3-4 week
old females if provided by PI
5. IVF: two proven breeder males age 2-8 month, 15-20 3-4 week old females if
provided by PI
Service Description Rederivation
1.
2.
3.
4.
5.
6.
7.
8.
purchase egg donors and recipient females (week 1)
superovulated donors and mate with stud males (week 2)
plug check for pseudopregnant foster mothers mated to vasectomized males
egg harvest
incubation of fertilized eggs O/N
overian transfer of successfully injected eggs (week 3)
weaning, ear tagging and tail biopsy of resulting offspring (week 8)
transfer of tail biopsies and offspring to the client (week 9)
Service Guaranties
Service Description Embryo Cryopreservation
1.
2.
3.
4.
5.
6.
purchase egg donors females (week 1)
superovulated donors and mate with stud males (week 2)
egg harvest
incubation of fertilized eggs O/N
slow freezing of 2-cell embryos in straws (week 3 and 4)
test thaw and transfer into pseudopregnant foster mothers mated to
vasectomized males (week 5)
7. weaning, ear tagging and tail biopsy of resulting offspring (week 10)
8. transfer of tail biopsies and transgenic founders to the client (week 11)
Service Description IVF
1.
2.
3.
4.
5.
purchase egg donors females (week 1)
superovulated donors (week 2)
harvest of eggs and sperm, IVF
incubation of fertilized eggs O/N
transfer into pseudopregnant foster mothers mated to vasectomized males
(week 3)
6. weaning, ear tagging and tail biopsy of resulting offspring (week 8)
7. transfer of tail biopsies and transgenic founders to the client (week 9)