etc3230-sup-0001-SupInfo-S1

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1
SUPPLEMENTAL DATA
Consoer, D.M., et al.
Toxicokinetics of perfluorooctane sulfonate (PFOS) in rainbow trout (Oncorhynchus mykiss)
Table S1. Kinetic parameters and tissue/plasma concentration ratios for fish used in renal elimination studies, determined
using a “renal plus other” model
Gender
Weight (g)
Kinetic parameters
VC (mL/kg)
k12 (1/d)
k21 (1/d)
k31 (1/d)
k41 (1/d)
VSS (mL/kg)
CLR (mL/d/kg)
CLT (mL/d/kg)
T1/2 (d)
AICa
BICb
Fish 1
Fish 2
Fish 3
Fish 4
Fish 5
Fish 6
Mean (SD)
♀
1346
♀
814
♂
1400
♂
1033
♂
934
♀
694
1037 (285)
29.9
0.2051
0.6995
0.00038
0.0141
132
0.27
10.36
48.0
7.89
8.06
46.3
0.1720
0.6348
0.00028
0.0216
217
0.31
24.37
31.6
7.31
7.49
54.6
0.3329
1.4817
0.00018
0.0583
297
0.24
76.60
11.8
7.37
7.54
74.5
0.1151
0.2671
0.00013
0.0259
247
0.24
46.51
26.6
7.60
7.77
45.6
0.1748
0.6186
0.00038
0.0134
207
0.42
15.12
50.1
8.51
8.68
36.8
0.4246
1.1402
0.00038
0.0145
135
0.33
13.11
46.6
6.64
6.81
47.9 (15.5)
0.237 (0.117)
0.807 (0.432)
0.00029 (0.00011)
0.025 (0.017)
206 (64)
0.30 (0.07)
31.01 (25.93)
35.8 (15.1)
7.55 (0.63)
7.73 (0.62)
0.83
0.49
0.05
1.71
0.75
0.04
0.70
0.27
NSc
0.70
0.29
NSc
0.87 (0.49)
0.41 (0.2)
0.05 (0.01)
Tissue/plasma concentration ratios at takedown
Liver
0.42
NSc
Kidney
0.22
NSc
Muscle
0.06
NSc
a
Akaike information criterion (AIC)
Bayesian information criterion (BIC)
c
NS = Sample lost or not collected
b
2
Figure S1. Kinetics of PFOS elimination to urine following a bolus intra-arterial
injection. Data and model simulations are shown for Fish 1. Simulations were obtained
using a model that includes a fitted “other” elimination term k4,1. In this and Figures S2S6 measured concentrations in plasma are shown as solid dots, while open triangles
denote the cumulative mass of PFOS eliminated in urine. Lines show the optimized fit of
model simulations to measured values: solid line – plasma; dashed line – urine.
3
Figure S2. Kinetics of PFOS elimination to urine following a bolus intra-arterial
injection. Data and model simulations are shown for Fish 2. Simulations were obtained
using a model that includes a fitted “other” elimination term k4,1.
4
Figure S3. Kinetics of PFOS elimination to urine following a bolus intra-arterial
injection. Data and model simulations are shown for Fish 3. Simulations were obtained
using a model that includes a fitted “other” elimination term k4,1.
5
Figure S4. Kinetics of PFOS elimination to urine following a bolus intra-arterial
injection. Data and model simulations are shown for Fish 4. Simulations were obtained
using a model that includes a fitted “other” elimination term k4,1.
6
Figure S5. Kinetics of PFOS elimination to urine following a bolus intra-arterial
injection. Data and model simulations are shown for Fish 5. Simulations were obtained
using a model that includes a fitted “other” elimination term k4,1.
7
Figure S6. Kinetics of PFOS elimination to urine following a bolus intra-arterial
injection. Data and model simulations are shown for Fish 6. Simulations were obtained
using a model that includes a fitted “other” elimination term k4,1.
8
Figure S7. Kinetics of PFOS elimination to expired branchial water following bolus
intra-arterial injection. Data and models simulations are shown for Fish 8. In this and
Figures S8-S11 measured concentrations in plasma are shown as solid dots, while open
squares denote the cumulative mass of PFOS eliminated to expired water. Lines show
the optimized fit of model simulations to measured values: solid line – plasma; dashed
line – water.
9
Figure S8. Kinetics of PFOS elimination to expired branchial water following bolus
intra-arterial injection. Data and models simulations are shown for Fish 9.
10
Figure S9. Kinetics of PFOS elimination to expired branchial water following bolus
intra-arterial injection. Data and models simulations are shown for Fish 10.
11
Figure S10. Kinetics of PFOS elimination to expired branchial water following bolus
intra-arterial injection. Data and models simulations are shown for Fish 11.
12
Figure S11. Kinetics of PFOS elimination to expired branchial water following bolus
intra-arterial injection. Data and models simulations are shown for Fish 12.
13
Figure S12. Kinetics of PFOS elimination to urine following a bolus intra-arterial
injection. Data and model simulations are shown for Fish 2. Simulations were generated
by adopting the average branchial elimination rate constant from branchial elimination
studies as the elimination rate constant k4,1. In this and Figures S13-S16 measured
concentrations in plasma are shown as solid dots, while open triangles denote the
cumulative mass of PFOS eliminated in urine. Lines show the optimized fit of model
simulations to measured values: solid line – plasma; dashed line – urine.
14
Figure S13. Kinetics of PFOS elimination to urine following a bolus intra-arterial
injection. Data and model simulations are shown for Fish 3. Simulations were generated
by adopting the average branchial elimination rate constant from branchial elimination
studies as the elimination rate constant k4,1.
15
Figure S14. Kinetics of PFOS elimination to urine following a bolus intra-arterial
injection. Data and model simulations are shown for Fish 4. Simulations were generated
by adopting the average branchial elimination rate constant from branchial elimination
studies as the elimination rate constant k4,1.
16
Figure S15. Kinetics of PFOS elimination to urine following a bolus intra-arterial
injection. Data and model simulations are shown for Fish 5. Simulations were generated
by adopting the average branchial elimination rate constant from branchial elimination
studies as the elimination rate constant k4,1.
17
Figure S16. Kinetics of PFOS elimination to urine following a bolus intra-arterial
injection. Data and model simulations are shown for Fish 6. Simulations were generated
by adopting the average branchial elimination rate constant from branchial elimination
studies as the elimination rate constant k4,1.
18
Figure S17. Kinetics of PFOS in trout plasma during a continuous waterborne exposure.
Data and model simulations are shown for Fish 14. In this and Figures S18-S21
measured values are shown as individual points. The fitted model simulation is shown as
a solid line.
19
Figure S18. Kinetics of PFOS in trout plasma during a continuous waterborne exposure.
Data and model simulations are shown for Fish 15.
20
Figure S19. Kinetics of PFOS in trout plasma during a continuous waterborne exposure.
Data and model simulations are shown for Fish 16.
21
Figure S20. Kinetics of PFOS in trout plasma during a continuous waterborne exposure.
Data and model simulations are shown for Fish 17.
22
Figure S21. Kinetics of PFOS in trout plasma during a continuous waterborne exposure.
Data and model simulations are shown for Fish 18.
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