ANIL KUMAR PANDEY
(M): +91 9974358458 (R): +91-02875-252696
B/27, Desai Park,
Nr. Shyamal Cross Road,
Vejalpur, Ahmedabad,
Gujarat – INDIA.
PIN CODE 380051
akpssm@gmail.com
Professional Snapshot
Working currently as a Senior Research Associate in a leading health care company
Zydus Cadila Healthcare Ltd. Able to work effectively in various innovative research
projects favoring overall development of firm. Good communication skills and a genuine
team player with good organizational and self-management skills.
Core competencies
- Mammalian Cell Culture
- Microbial Fermentation
- Cell banking
- GMP exposure
- Significant Achievements
- Project coordinato
Work Experience
Zydus Cadila, Ahmedabad-Gujarat
Duration: 6 years 3 months, January 2008 – Till Date
Senior Scientific Assistant: From January 2008 to March 2010.
Research Associate: From April 2010 to March 2012.
Senior Research Associate: From April 2012 to March 2014.
Senior Associate Scientist : From April 2014 to Till Date.
Description: Zydus Research Centre is one of the best R&D centre in India where
different projects are ongoing mainly dealing with novel drug delivery systems, process
development of recombinant proteins, genomics and chemical sciences.
Responsibilities:
Mammalian cell culture:-
Media optimization, Process development and Scale up for Clone Expressing

Anti CD20 Chimeric Mab protein in Spinner flask to 2 L to 30 L to 50 L to 200 L
(Sartorius Stedin) Disposable Bioreactor.

Anti Rabies Mouse Mab Cocktail protein (Supported by WHO) in Spinner flask to 2
L to 30 L bioreactor.

TNF inhibitor fully Human Mab protein in Spinner flask to 2 L to 30 L to 200 L
(Sartorius Stedin) Disposable Bioreactor.

HER2/NEO Humanized Mab protein in Spinner flask to 2 L to 30 L to 50 L
(Sartorius Stedin) Disposable Bioreactor.

Anti Angiogenesis Humanized Mab protein in Spinner flask to 2 L to 30 L
Bioreactor.

Second Generation Erythropoiesis Stimulating protein in Spinner flask
to 2 L to
30 L Bioreactor.

Follicle Stimulating Hormone in Spinner flask

TNF inhibitor fusion protein in Spinner flask to 2 L Bioreactor.
to 2 L to 30 L Bioreactor.

Third Generation Streptokinase protein in Spinner flask to 2 L Bioreactor.

Execution of batches of Anchorage dependant cell line as per standard protocol.

Scale of process of anchorage dependant cell line.
Microbial Fermentation:-

Successful handling of research projects in Biotechnology Department.

Upstream process development of recombinant proteins in E. coli and Picchia Pastoris
strains in 30L fermentor.

Screening experiments for high protein producing strains.

Carrying out In-process quality control (IPQC) tests.

Preparation of process SOP’s of the product.

Preparation of instrument SOP’s.

preparation of MFR and BPR.
Cell banking:
Preparation of Developmental, Master and Working cell banks for
different recombinant clones of CHO cell line as well as hybridoma cell
line.

Preparation of SOP’s, Protocol’s and CBPR (Cell bank preparation
record) document with involvement of Quality Assurance department.

Record keeping and maintenance of different project Cell banks as per
ICH guidelines.

Issuance of cell banks to manufacturing with accordance with in house
quality system.
GMP exposure:
Execution of different project batches for generation of Clinical Trial
material following Good Manufacturing Practices.

Brief
exposure
to
current
Good
Manufacturing
Practice
during
technology transfer of product.

Preparation of Technology transfer documents with key approval from
Manufacturing Quality Assurance.

Preparation of process related protocols and SOP’s for final product.
Significant Achievements:
Project Coordinator for one of Monoclonal Antibody project and lead
the project to preclinical stage.

Optimization of various process parameters in multi bioreactor
(250 ml) Aplicon system.

Successfully scaled up process from culti flask scale to 2L to 20L to
STR 50L and STR 200L Disposable bioreactor.

Developing processes and scale up of recombinant biotherapeutic
protein using mammalian cell culture systems (CHOS & CHOM) and
Hybridoma cell line.

Successfully scaled up process for manufacturing a biotherapeutic
protein from lab scale to manufacturing scale.

Successful designed and established state of art cell culture facility.

Adherent culture process optimization from T-flasks to Roller bottles.

Suspension
culture
process
and
feed
optimization
at
T-flasks,
cultivation flask, shake flask, spinner flask and bioreactor level.

Two fold improvement in cell density at spinner flask and then scaled
up to bioreactor batches.

Three to four fold improvement in volumetric yield at spinner flask and
then scaled up to bioreactor batches.

Media
Preparation,
Sterile
Filtration
processes
involving
various
filtration modules.

Cell count, trypsinization, passage and routine maintenance of both
Adherent and suspension cell lines.

Perfusion technique for continuous culture.

Fibra cell disc technique for adherent cell line.

Phenotypic and Expression stability of Clones of respective projects.

Perform ELISA for suspension culture Biotherapeutic protein.

Sterility testing and various microbiological techniques.

Supervising and training cell culture techniques to fresher.

Knowledge of MS-Office, graph pad, softmax pro and SAP.
Project Coordinator:
Planning of experiments for project with coordination with different
groups.

Discussion and interpretation of data.

Compilation of data and presentation of data to higher management in
the monthly as well as quarterly review.
Personal Details
Date of Birth: 10th August, 1984
Gender: Male
Marital Status: married
Languages Known: English, Hindi, Gujarati
Hobbies: Surfing Internet for widening knowledge, Chess, Cricket, Football, Movies & Music.