teacher background information FSP13 Soil analysis Soils Why would soils be basic? Soils are made up of many things. It is a complex mixture of minerals, plant and animal matter and tiny particles of man-made substances such as glass, paint, concrete and other materials. The contents of soil can vary greatly between one site and another. For the same reason that some soils have acids added to them, soils are made basic by having lime and other things added to them. This is because some plants prefer an alkaline environment. Soils may be naturally basic too. Dry climates generally have more basic soils. This is because places that receive little rain are high in carbonate (alkaline) because it has not been leached (washed) out of the soil by water. Soil is also made up of a variety of different sized particles. If a soil is made up of mainly small particles then it will be a fine soil. But if a soil is composed of larger particles it would be a coarser soil. Even clues like these can help in an investigation. How to test soils pH? Forensic soil analysis usually begins with a visual and microscopic examination. From there various tests are done to ascertain the colour, consistency, pH and mineral content. Any foreign substances that may be present (ie hair, plant matter) are also looked for as these may provide important clues for the investigation and may help explain the origin of the soil. There are two easy ways you can test soil pH. Soil pH testing kits are available from gardening and hardware shops. A small sample of soil is collected, and then after a chemical reaction with a ‘universal’ pH indicator (one that has a different colour for every point on the pH range) the sample can be matched to a colour chart The pH of soils may also vary. This is important as nutrients in the soil are only available to the plant within a certain pH range. A soil of pH 4.0 is extremely acidic, while one of pH 8.5 is very alkaline. You can add water to the soil and if the chemicals that are making it acidic or basic are soluble in water, then you can test the water to find out its pH. Why would soils be acidic? Some plants (azaleas) prefer an acidic environment so adding materials such as peat, manure and compost will make the soil more acidic and make azaleas grow better. Soils may also be naturally acidic. Moist climates generally have more acid soils. This is because high rainfall causes soils to weather faster. Basic chemicals such as calcium and magnesium, which are essential to living organisms, are leached from the soil leaving behind more stable materials rich in iron and aluminium and more acid. Action by humans such as mining, farming, construction site development, and similar events that remove topsoil can make the soil more acidic. Forensic investigations: Soil analysis (teacher information information) FSP13 | revised May 2013 | © The University of Western Australia page 1 Licensed for NEALS The plants involved in the classroom crime scene There are two plants involved: an azalea that is in a pot on the teacher’s desk and a pink hydrangea that is planted outside the broken classroom window. The common garden variety hydrangea (Hydrangea macrophylla) is one of nature’s very own pH indicators: pink flowers are produced at pH values of 6.8 or higher, and blue flowers at pH 6.0 or below. Most flowers are coloured by their genetic material however hydrangeas, unlike most flowers, are coloured by the soil they grow in. Azaleas (Rhododendron spp) are plants that need to be grown in soil with a pH of 5.5. In the crime scene, the soil on the desk, on the carpet near the window and on the ledge in front of the window should be a low pH (pH 5) indicating that the soil has come from the azalea pot and NOT from outside. If the soil on the carpet and ledge came from outside it would have a higher pH as the hydrangea is pink indicating that the soil pH would be greater than pH 6.8. The perpetrator of the crime has placed the soil on the carpet and the window ledge to make it look like they entered from the outside garden via the broken window. photos: A. Dook References http://www2.hawaii.edu/~nvhue/acid.html http://www.ext.vt.edu/pubs/trees/430027/430-027.html Forensic investigations: Soil analysis (teacher information information) FSP13 | revised May 2013 | © The University of Western Australia page 2