ESF-CUC PhD Studentship Opportunity The perfect soil: an investigation of the chemical characteristics needed for healthy, artificial soils at the Eden Project, Cornwall Dr Mark Fitzsimons, University of Plymouth; Dr Tim Pettitt, Eden Project; Dr Gavyn Rollinson, Camborne School of Mines, University of Exeter Description of the study: Soil consists of loose weathered rock debris and decayed organic material. Mature soil with a robust capacity to retain plant nutrients normally takes thousands of years to form. This project looks at manufactured soil, building on expertise accumulated by the Eden Project (EP), with the overall aim to produce artificial soil from waste materials that can maintain a reservoir of slow-release nitrogen to sustain growth without the high demand for fertilizer application associated with the majority of manufactured soils currently used at EP. The project will begin by characterizing materials used by EP for mineral content and structure, nitrogen content and speciation (inorganic or organic nitrogen) together with some extant EP soils of varying properties. Soils will then be made and weathered under different climate regimes and treatments, in bioreactors (e.g. soil columns) to measure changes in nitrogen speciation over time, and the role of mineralogical structure in nitrogen retention. The most promising soils will be further refined after reviewing the initial data, to improve performance where possible, and then tested under different biome conditions and also simulated extreme events, such as desiccation and intense rainfall, to assess the robustness of the soil nitrogen reservoir. The project brings together scientists with expertise in nitrogen cycling and speciation, mineralogy and soil manufacture and ecology, applying state-of-the-art analytical techniques that will enhance the ability of the EP to produce a sustainable and commercial product. This will bring EP, and the locality, significant benefits in terms of personnel skills, sustainability and commercial potential. The PhD student will be trained in the analysis of nutrients and metals, and will contribute to method development through design of the bioreactors and extraction techniques. Entry requirements: This project would be suitable for students holding, or expecting to achieve, at least a 2:1 honours degree in chemistry, environmental science, earth science, soil science or another related discipline. Funding: The studentship is supported for three years from 1 October 2011 and includes full Home/EU tuition fees and a stipend of £13,590 p.a. (tax free). Application procedures: For an application form, and full details on how to apply, please visit www.plymouth.ac.uk/postgraduate. In order to apply you will need to complete the standard postgraduate application form along with a covering letter detailing suitability for the individual studentship. In section 9 of the application form (Funding Information) please ensure that you declare that you are applying for an ESF-CUC Studentship. On completion send your application form to Catherine Johnson, Faculty of Science and Technology Research Office, Room A103, Portland Square, Plymouth PL4 8AA or e-mail catherine.johnson@plymouth.ac.uk. Further information on the terms and conditions of a PhD at the University of Plymouth can be found on www.plymouth.ac.uk/graduateschool. For an informal discussion please contact Dr Mark Fitzsimons at mfitzsimons@plymouth.ac.uk. Closing date for applications: 12 noon, Friday 3 June 2011 Further Particulars Soil formation occurs and proceeds at a faster rate in the presence of biota. Although plants can build organic molecules by photosynthesis, elements other than carbon, hydrogen and oxygen must be obtained from the soil. The ions needed for building organic molecules are sequestered in the crystal structure of minerals and soil becomes a source of nutrients as the minerals weather and bedrock breaks down. Decomposition processes, favoured by the ability of soils to hold moisture and moderate temperatures, are responsible for recycling nutrients. By promoting decomposition while retaining nitrogen and other nutrients, the soil prevents rapid loss of essential elements to groundwater and rivers. This results in a net accumulation of bioavailable nutrients in the soil and enhanced productivity at all trophic levels. The Eden Project manufactures its own soils for use in its biomes, by recycling raw materials sourced in Cornwall. The performance of soils used in the biomes is variable and the reasons for this are not fully understood. These soils all follow a similar basic recipe, using china clay waste sand as the primary inorganic fraction. Their capacity of the soil to hold plant nutrients was not a prime consideration as it was initially planned to maintain plant nutrition by carefully-controlled fertilizer applications. However, after 10 years of hugely variable environmental conditions, plantings, fertilizer practices, incidental introductions of micro- flora and micro- and meso-fauna, as well as general husbandry (mulching and allowing natural organic matter turnover), some EP soils are giving better performance in terms of nutrient storage than was originally anticipated, whilst others are still quite poor. This range of soils and environments provides an opportunity to look more closely at the possibility of developing ‘low-maintenance’ artificial soils in terms of their nitrogen reservoir, cation exchange capacity and ability to retain and recycle nitrogen compounds. Interestingly, china clay waste sand appears to be an excellent base for creating a soil/growing medium. The capacity for detailed mineral and nutrient analyses, especially nitrogen, is very limited at EP and access to advanced analytical facilities in a focussed project would be hugely advantageous. This project will be framed around the following research question: “How does soil composition effect the bioavailability of its nitrogen reservoir and, therefore, its ability to become a self-sustaining soil?” We have these Aims & Objectives: Aim: To develop a fertile, artificial soil with a large reservoir of slow-release nitrogen that meets the nutrient requirements of the soil. Objectives: 1. To characterise the raw materials currently used to manufacture soils at the EP using appropriate analytical techniques (e.g. microscopy, elemental analysis). 2. To construct soil bioreactors where the performance of soils can be measured in terms of key characteristics (e.g. nutrient retention, organic content). 3. To use different amendments on the soils being tested to see how this affects the sustainability of the nitrogen reservoir. 4. To measure the performance of the differently-amended soils under the biome conditions used at the EP. 5. To produce a protocol for the manufacture of an N-rich soil that can be utilised from waste materials available in Cornwall and which has wide application for local projects. The project brings together experts in nutrient research and mineralogical studies, to build on the biological/microbiological expertise available at the Eden Project (EP) and advance understanding of the characteristics of the soils that the EP has manufactured to date, relating this to their functionality. Description of research The project will progress in logical stages dependent on the experiments and analyses needed to logically address the research question and meet the aims and objectives of the project. Outline Work programme: Year 1 Months 0-6: Training in sampling and laboratory techniques (SKALAR nutrient analyzer, Shimadzu TOC/TN analyzer, ammonium analysis by fluorescence, metal digest using lithium borate; metal analysis by ICP-AES and ICP-MS; Months 6-12: Characterisation of raw materials and established soils to determine mineral structure and nitrogen bioavailability; microscopy analysis of samples to select samples of interest for QEMSCAN high resolution structural analysis End of Year 1: Review of analytical data for preparation of soil preparation and treatment experiments in Year 2 Year 2 Months 12-18. Set up bioreactors using different material mixtures and amendments as agreed at review meeting, and perform time-resolved sampling according to rate of soil maturity; test nitrogen reservoir of amendments used; Months 18-24. Select best performing soils for analysis of mineral structure using QEMSCAN for high-resolution mineralogical analysis of structure; End of Year 2: Review of analytical data to determine further experiments necessary (e.g. refinements of soil material mix, amendments, testing of soil in different biomes) Months 24-30: Accumulation of further experimental data as guided by Year 2 Review; Months 32-36 Thesis write-up. Key Aspects of Research Training for the Student: The PhD student will be trained in the analysis of nutrients and metals, and will contribute to method development through design of the bioreactors and extraction techniques. While the experimental work will be carried out the the EP, both the UoP and CSM analytical facilities are close by so that the students can easily organise sample analysis, having only a short distance to travel to both institutes. This is a full collaboration with the Eden Project and CUC partner (Camborne School of Mines, University of Exeter), with the research question being guided by the experience of the EP in their manufacture and application of soils in the biomes. Dr Tim Pettitt, is a microbiologist and Assistant Curator of Science Team at the EP and has been conducting research into the biological colonisation of artificial EP soils for 6 years. Dr Rollinson is an expert in the use of QEMSCAN, an advanced automated scanning electron microscope that analyses inorganic samples such as rock and soil (the only instrument of its type in a university in Europe and one of four in a university globally). Dr Fitzsimons is an expert in nutrient (particularly nitrogen speciation) and organic geochemistry and has developed analytical techniques to study nitrogen in a range of challenging environmental matrices.