Supplementary Table I. Yeast strains used in this study
Strain
RS453
Ds1
Sac3∆
Sac3∆
Sac3∆ (G1)
Sac3∆ SUS1-GFP
Sac3∆ THP1-GFP
SUS1-13xmyc
SUS1-TAP THP1GST
CDC31-TAP
SPC42-GFP
Cdc31∆
Cdc31-115
Cdc31-54
tetO::CDC31
SUS1-TAP THP1GST sac3∆
THP1-TAP SUS113xmyc
Sus1∆
tetO::CDC31
SUS1-GFP
genotype
MAT a/; ade2-1/ade2-1; his3-11,15/his3-11,15; ura3-52/
ura3-52; leu2-3,112/leu2-3,112; trp1-1/trp1-1
MAT  leu2; trp1; his3; ura3; ADE2; ADE3; wildtype
MAT a; his3∆1; leu2∆0; met15∆0; ura3∆0; sac3::kanMX4
MAT ; his3∆1; leu2∆0; lys2∆0; ura3∆0; sac3::kanMX4
MAT a; his3; leu2; ura3; trp1; ade2; sac3::kanMX4
MAT  leu2; trp1; his3; ura3; ADE2; ADE3; sac3::kanMX4;
SUS1-GFP::TRP1
MAT  leu2; trp1; his3; ura3; ADE2; ADE3; sac3::kanMX4;
THP1-GFP::TRP1
MAT  leu2; trp1; his3; ura3; ADE2; ADE3; SUS113xmyc::TRP
MAT a; ade2-1; his3-11,15; ura3-52; leu2-3,112; trp1-1; SUS1TAP::TRP; THP1-GST::HIS
MAT  leu2; trp1; his3; ura3; ADE2; ADE3; CDC31TAP::TRP
MATa ura3-52 leu2s1 his3s200 trp1s63 SPC42GFP::KanMX6.
MATa ura3-52 lys2-801amber ade2-101ochre trp1s63
leu2s1 his3s200 scdc31::HIS3
leu2-MET3-CDC31-LEU2
MAT  leu2; his3; ura3; ade2; cdc31-L45S::ura
MAT  leu2; his3; ura3; ade2; cdc31-D144N::ura
MAT  leu2; trp1; his3; ura3; ADE2; ADE3;
tetO::CDC31::kanMX4
MAT a; ade2-1; his3-11,15; ura3-52; leu2-3,112; trp1-1; SUS1TAP::TRP; THP1-GST::HIS;
MAT ; ura3; leu2; trp1; his3; THP1-TAP::TRP1-KL; SUS113xmyc::HIS3; sac3::kanMX4
MAT a; his3∆1; leu2∆0; met15∆0; ura3∆0; sus1::kanMX4
MAT  leu2; trp1; his3; ura3; ADE2; ADE3;
tetO::CDC31::kanMX4 SUS1-GFP::TRP
reference
Euroscarf
Euroscarf
Fischer et al., 2002
Rodriguez et al., 2004
Fischer et al., 2002
this study
this study
this study
Pereira et al., 2001
Spang et al., 1995
Ivanovska and Rose, 2001
Ivanovska and Rose, 2001
this study
this study
Rodriguez et al., 2004
Rodriguez et al., 2004
this study
Supplementary Table II. Plasmids and plasmid constructions
plasmid
pGALPATG1L
pNOPPATA1L
pNOPGFPA1L
pGALPATG1LSAC3/SAC3-C(563-1301)
pNOPPATA1L -SAC3
pNOPGFPA1L –SAC3 /C
pGALPATG1L-SAC3608-1301/696-1301/7331301/851-1301
pGALPATG1L-SAC3CID
pGALTAPG1L-SAC3
563-1301/733-1301/8511301/CID
pNOPPATA1LSAC3/SAC3-C(5631301)
pNOPPATA1L-SAC3608-1301/696-1301/7331301/851-1301/CID
pNOPGFPA1L-SAC3608-1301/696-1301/7331301/851-1301/CID
PRS426-CDC31
pRS316-CDC31-GFP
(pSM875)
pNOPPATA1LSAC3∆CID
pNOPGFPA1LSAC3∆CID
Rpl25-GFP
Rps2-GFP
pRS426-NLS-NES2xGFP
construction
reference
Künzler et al., 2000
Hellmuth et al., 1998
Hellmuth et al., 1998
Fischer et al., 2002
Fischer et al., 2002
Fischer et al., 2002
SAC3 bp 1822-3903/2086-3903/2197-3903/2551-3903 were
inserted into NcoI and BamHI sites of pGALPATG1L
this study
SAC3 bp 2197-2582 were inserted into NcoI and BamHI sites of
pGALPATG1L
ccatggagaagagaagatggaaaaagaatttcatagccgtctcagcagccaaccgctttaa
gaaaatctcatcctccggggcacttgatgccatgg sequence was inserted into
NcoI site of the corresponding pGALPATG1L plasmid
this study
this study
Fischer et al., 2002
SAC3 bp 1822-3903/2086-3903/2197-3903/2551-3903/2197-2582
were inserted into NcoI and BamHI sites of pNOPPATA1L
this study
PstI fragment of the corresponding pNOPPATA1L plasmid was
inserted into PstI site of pNOPGFPA1L
this study
Not1 restriction site was introduced before the stop codon of
CDC31. The Not1-fragment of pYM12 containing GFP-KanMX6
was then inserted into the Not1 site.
SAC3 bp 1-2199 was inserted into NcoI site of pNOPPATA1LSAC3 851-1301
PstI fragment of the pNOPPATA1L-SAC3∆CID plasmid was
inserted into PstI site of pNOPGFPA1L
Geier et al., 1996
this study
this study
this study
Hurt et al., 1999
Fischer et al., 2002
Stade et al., 1997
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