Table S3 The genotype and genotype effect of marker and QTL for combining ability and heterosis with multiple alleles at each locus in DH population MM mm Genotype in DH population MMQQ MMqq mmQQ mmqq Genotype effect in DH population a a a a Genotype frequency population 1 (1 r ) 2 1 r 2 1 r 2 1 (1 r ) 2 *Genotype in TC population MMQ1Q MMQ1q mmQ1Q mmQ1q *Genotype effect in TC population g1 g1' g1 g1' 1 (1 r ) 2 1 g1 (2 a1 a) 2 1 1 ( g1 g1 ) (G G ) 2 2 1 r 2 1 r 2 1 g1 (2 a1 a ) 2 1 1 ( g1 g1 ) (G G) 2 2 1 g1 (2 a1 a) 2 1 1 ( g1 g1 ) (G G ) 2 2 1 (1 r ) 2 1 g1 (2 a1 a ) 2 1 1 ( g1 g1 ) (G G) 2 2 … … … … … #Genotype in TC population MMQk Q MMQk q mmQk Q mmQk q #Genotype effect in TC population d a d a *Genotype population frequency in in DH TC *Genotype effect in Hmp data set *Genotype effect in Sca data set #Genotype population frequency in TC #Genotype effect in Hmp data set #Genotype effect in Sca data set #Genotype effect in Gca data set 1 (1 r ) 2 1 g k (2 ak a ) 2 1 1 ( g k g k ) (G G ) 2 2 1 (G G ) 2 1 r 2 1 r 2 1 g k (2 ak a ) 2 1 1 ( g k g k ) (G G) 2 2 1 (G G ) 2 1 g k (2 ak a ) 2 1 1 ( g k g k ) (G G ) 2 2 1 (G G ) 2 1 (1 r ) 2 1 g k (2 ak a ) 2 1 1 ( g k g k ) (G G) 2 2 1 (G G ) 2 MM and mm denote the two different genotype of molecular marker M; Q and q denote two alleles of QTL in DH population, Qi (i=1~k) represents the multiple alleles of QTL in tester; r represents the recombinant value between molecular marker M and QTL in DH population; μidenotes the overall mean value. a, a1 and ak denote the additive effect of different allele; gi and gi’ (1~k) denote the genotypic value of the homozygote and heterozygote of QTL, respectively. G G 1 k gi . For the RI population, the expectations were similar to those in the DH population except for r, which was replaced by k i 1 respectively. The rm and rm were /(1 2 r ') 2 rm m and 1 k gi , k i 1 4 rm /(16 rm ) recombinant values for two RI populations (selfing population and sib-mating population), respectively(Hu et al. 2002). *When the genotype of QTL is Q1Q1 in tester # When the genotype of QTL is QkQk in tester ,