Phytoneer
ImpactVector™
Vector information: Cytoplasmic
expression or expression with
native signals
By default genes or gene fragments without pre-pro targeting peptides will
express in the cytoplasm and this basic vector will help to achieve just that. It does
provide the option of adding a tag to your protein of interest. The vector is also
your choice if you want to express your gene with its own native targeting
information.
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Phytoneer
ImpactVector™
Vector information: Secreted expression
Secreted expression is important for proper formation of disulphide bonds,
glycosylation, etc., and is the first step in the sorting of proteins to the ER, Golgi,
transport vesicles, the vacuole, peroxisomes etc. The default pathway is secretion
out of the cell into the cell wall and apoplast. Various sorting and retention signals
determine the actual protein destination (see ImpactVector1.3). The vector can be
used to change the targeting of the mature part of a protein to a secreted
destination. The signal peptide used in this construct was derived from sea
anemone equistatin (Outchkourov et al. 2003 Planta: 216: 1003; Outchkourov et
al. 2003 Plant Physiology 133: 379).
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Phytoneer
ImpactVector™
Vector information: Endoplasmic reticulum
expression
By adding the well known KDEL retention or sorting signal to vector
ImpactVector1.2 we created vector ImpactVector1.3. With this vector proteins are
retained in the ER and do not go downstream into Golgi. ER retention has a
practical advantage in that it has been reported to improve expression levels
5-fold or more. The main reason for this appears to be that the ER contains lower
concentrations and/or different proteases responsible for post translational
degradation of expressed proteins (Outchkourov et al. 2003 Plant Physiology 133:
379-390)
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Phytoneer
ImpactVector™
Vector information: Chloroplast expression
Targeting to the chloroplast brings a protein into a completely different cellular
environment capable of different metabolic and catabolic functions.
ImpactVector1.4 vector uses the secretion signal of the Chrysanthemum
morifolium small subunit protein to deliver your protein into the chloroplast stroma.
The protein is fused to the first 11 amino acids of the rubisco protein in order to
allow proper processing of the signal peptide (Wong et al., Plant Molecular
Biology 20: 81-93 (1992)). The signal peptide contains a natural intron from the
RbcS gene.
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Phytoneer
ImpactVector™
Vector information: Mitochondrial expression
Targeting to mitochondria brings a protein to a completely different subcellular
environment capable of different metabolic and catabolic functions. The
ImpactVector1.5 vector uses the yeast CoxIV secretion signal which was shown
to deliver proteins in the mitochondrial matrix. The protein is fused to the first 4
amino acids of the yeast CoxIV protein in order to allow proper processing of the
signal peptide (Kohler et al. Plant J 11: 613-621 (1997)).
PRI ImpactVector™ 台灣區總代理
www.impactvector.com
艾特克生物科技股份有限公司
TEL:02-29072616 FAX:02-29072816
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