Title and legends to supplementary figures
Supplementary Figure S1. Aβ accumulation in CBKOTg mice was not different in the frontal
cortex, compared to that of Tg mice.
(a) Representative cortical regions stained with anti-calbindin-D28k (red) and anti-Aβ (4G8;
green) commonly showed Aβ accumulation and differential expression of CB. Total Aβ plaque
burdens in the cortex were not different between the groups, similar to the subiculum. (b) The
percentage of the areas occupied by 4G8 were quantified in cortical layers 1-3 and 5 (n = 4 Tg
and CBKOTg mice, respectively).
Supplementary Figure S2. Proteins that significantly changed in the table 1 were validated by
western blotting between Tg and CBKOTg mice.
(a) Expression levels of p35, PKB/Akt, p-IκB, neurofilament 200, and dystrophin were analyzed
by western blotting in the subiculum Tg (n = 4) and CBKOTg mice (n = 4). Tubulin was a
loading control. (b) p35 did not differ between Tg and CBKOTg mice and PKB/AKT decreased
in subiculum of CBKOTg compared to Tg mice. (c) p-IκB, neurofilament 200, and dystrophin
tended to decrease in CBKOTg mice compared to Tg mice. *P < 0.05, CBKOTg vs. Tg mice.
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Title and legends to supplementary tables
Table. S1 31 DEPs were identified from the antibody array analysis of the subiculum between
CBKOTg and Tg mice. P-values represent overall p-values computed by the integrative
statistical testing method (Materials and Methods). A smaller P-value (or a larger fold change)
indicated a more significant change in protein expression by CB depletion. The signs of fold
change denote up- (positive) and down-regulation (negative) in CBKOTg mice, compared to Tg
mice. The DEPs were categorized into several functional groups by grouping the DEPs with the
same GOBPs.
Table. S2 269 DEPs identified from LC-MS/MS analysis. Protein IDs (IPI, EntrezID, and
symbols) were shown. P-values represented the significance of proteins being differentially
expressed by CB depletion. A smaller P-value (or a larger SPI) indicated a more significant
change in protein expression by CB depletion. The values of the SPI can range from -1 to +1.
SPI = 0 implies that the abundance of the corresponding protein was equal between CBKOTg
and Tg mice, while SPI = 1 implies that the abundance was significantly higher in CBKOTg
mice, compared to Tg mice. The signs of SPI denote up-(positive) and down-regulation
(negative) in CBKOTg mice, compared to Tg mice.
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Table. S3 GOBPs were represented by 269 DEPs identified from LC-MS/MS analysis. The
GOBPs with P-value < 0.01 were selected. P-values represented the significance of the
corresponding GOBP being enriched by the 269 DEPs. A smaller P-value indicated a more
significant enrichment of the GOBP by the DEPs.
Table. S4 DEPs were involved in the selected GOBPs. Protein counts represent the number of
DEPs involved in the selected GOBPs: ‘union’ means the total number of DEPs involved in the
GOBP, and ‘up-regulated DEPs’ and ‘down-regulated DEPs’ mean the numbers of up- and
down-regulated proteins involved in the GOBPs, respectively. The symbols of the proteins
involved in each GOBP were also included.
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