Biological Treatment of Municipal Wastewater L.M. Chu Department of Biology Treatment of municipal wastewater usually involves biological processes such as the activated sludge system in the secondary stage after preliminary screening which remove coarse particles and primary sedimentation which settles out suspended solids. These secondary treatment steps are generally considered environmental biotechnologies which harness natural self-purification capacity in enclosed reactors for the biodegradation of organic matter and bioconversion of soluble nutrients in the wastewater. This talk attempts to introduce the secondary biological treatment of wastewater from an environmental biotechnology viewpoint using activated sludge process as an example. The aim of wastewater treatment, the operational requirement of the bioreactor, factors affecting treatment efficiency and the current development for biological nutrient removal will be discussed. Municipal facilities for wastewater treatment in Hong Kong will also be illustrated. Activated sludge process is a suspended growth system in which free living organisms are exploited to breakdown the organic matter in the wastewater. Another type, which is quite common overseas for smaller communities, is the trickling filters which utilize surface attached organisms for wastewater purification. In the activated sludge process, the wastewater is treated aerobically by a microbial consortium dominated by heterotrophic bacteria which are flocculated in the mixed liquor of the aeration tank to form discrete clumps of microorganisms. Protozoa and rotifers which enhance floc formation and effluent clarification are also present in significant numbers. Aeration is one of the key operational designs which contributes to the efficient degradation of organic matter (BOD removal). This is done by bottom air blowers such as porous diffusers, or surface aerators, which oxygenate the mixed liquor for aerobic degradation. Other operational parameters which affect process efficiency include mixed liquor suspended solids, organic loading, food to microorganisms ratio, sludge residence time and sludge recycle ratio. Effluent quality depends not only on the treatment process in the aeration tank, but also on the separation of the flocs from the treated effluent by a final sedimentation tank. The latter is dependent on floc settleability which is primarily affected by the density of the flocs, as in the case of sludge bulking caused by filamentous bacteria such as Sphaerotilus natans which interferes with the performance of many sewage works both locally and overseas. Another operational problem commonly encountered is bacterial foaming which is caused by the excessive growth of filamentous actinomycetes such as Nocardia amarae. The control measures for these problems will be discussed. Although activated sludge system is an aerobic process, modern wastewater treatment plants which employ activated sludge system usually have anaerobic unit(s) or zone(s) which allow biological denitrification to take place for nitrogen removal. This anoxic-oxic (A/O) system is essential as conventional activated sludge process is not effective in removing nutrients, which inevitably results in the eutrophication of the water bodies receiving the effluent. Sewage treatment plants in Hong Kong all use M-5-1 activated sludge process with up to 5 alternate aeration zones (modified Bardenpho process). References Grady, C.P.L., Daigger, G.T. & Lim, H.C. (1999) Biological Wastewater Treatment. 2nd Ed. Marcel Dekker, New York. (Chapters 10, 11, 19 & 20) Gray, N.F. (1989) Biology of Wastewater Treatment. Oxford Science Publications, Oxford. (Chapters 2 & 4) Bitton, G. (1994) Wastewater Microbiology. Wiley-Liss, New York. (Chapters 8, 9 & 10) Forster, C.F. and Wase, D.A.J. (1987) Environmental Biotechnology. Horwood, Chichester. (Chapters 1, 2 & 4) Ellis Rehm, H.J. and Reed, G. (1986) Biotechnology. Vol. 8 Microbial Degradations. VCH, Weinheim. (Chapters 2 & 3) Questions for revision 1. Why is the aeration tank of a sewage works considered a bioreactor? 2. What is common between biological wastewater treatment systems and natural purification process? 3. What are the factors affecting the quality of the effluent discharged from an activated sludge process? 4. Discuss the operational problems commonly encountered in activated sludge treatment plants. 5. Aerobic treatment system is not necessarily a solely aerobic process. Discuss. 6. How is nitrogen removed from the wastewater in an activated sludge plant? M-5-2