NUS Institutional Animal Care and Use Committee
000/15
Date revised: Jun 2015
Form expiry date: 31 Dec 2015
Procedure A
Surgery and Management of Surgical Pain and Distress
Species:
Number of animals:
Complete this procedure form for each surgical procedure and/or species.
Note: 1. Survival surgery for all species must be performed in a pre-approved area properly
designed and maintained to enable adherence to the principles of aseptic technique. Surgery on
non-rodent mammals must be done in a dedicated surgery area approved by the IACUC.
2. Investigators are expected to use pharmaceutical-grade medications whenever they are
available, even in acute procedures. Non-pharmaceutical-grade chemical compounds should only be
used after specific review and approval by the IACUC for reasons such as scientific necessity or
non-availability of an acceptable veterinary or human pharmaceutical-grade product. Cost savings
alone are not an adequate justification for using non-pharmaceutical grade compounds in animals.
1. Surgical procedure is:
Non-survival
Survival (Aseptic technique must be described for all species)
2. Location of surgical procedure:
Procedure room in CM vivarium.
Licensed procedure room outside CM vivarium. Please complete the table below:
Building:
Room number:
3. Name of person(s) performing the surgical procedure:
Note: Personnel performing surgery should have attended the ‘Aseptic Surgery’ handson session of RCULA
(a) Relevant experience with the
animal model
Name
(b) Relevant experience with the
procedure
4. Describe the surgical procedure and the aseptic techniques (in the case of
survival surgery)
Full details of the surgical procedure should be given. For survival surgery, full details of aseptic
techniques should also be given. Description of aseptic techniques should include preparation
of the surgical space, the surgeon(s) and the animal(s), and sterilisation of instruments.
Note: If substances (e.g., drugs, cells, implants, etc) are administered by or during the surgical
procedure, details of the substances should be provided in the relevant sections of Procedure B
form.
5. Methods of induction and maintenance of anaesthesia:
Agent
Dose
Tranquilisation
Induction
Maintenance
Analgesia
Procedure A
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Volume
Route
Frequency
NUS Institutional Animal Care and Use Committee
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Antibiotics
6. What are the potential complications that may arise during the procedure and
how will they be addressed?
(a) Potential complication:
(b) How the complication will be addressed:
7. Will the procedure involve the use of neuromuscular paralytics?
Neuromuscular paralytics should not be used without anaesthesia.
No.
Yes. Please describe anaesthetic dose/route/frequency AND criteria for judging depth of
anaesthesia
For Survival Surgery, please answer the following questions:
8 Where will the post-operative care be provided?
Building:
Room number:
9. What are the potential complications that may arise as a result of the procedure
post-operatively, and how will they be addressed? (Include analgesics, antibiotics and
monitoring of fluids and body temperature.)
(a) Potential complication:
(b) How the complication will be addressed:
(c) Post –operative plan:
Agent
Dose
Volume
Route
Frequency
Duration
(days)
Analgesic
Antibiotic
10. How many days will the animals be examined daily after surgery?
11. Will any animal undergo more than one survival surgical procedure?
Note: Only one survival surgery may be performed per animal, unless indicated on an approved
protocol.
No.
Yes. Please provide an appropriate scientific justification. Multiple survival procedures on
the same animal are not permitted, unless the procedures are essential components of
the same project. Include in your justification any pain, distress or functional deficit that
may result and describe how such distress will be minimised.
Procedure A
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NUS Institutional Animal Care and Use Committee
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Procedure A
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