Professor Dr. Esther Middelkoop
Association of Dutch Burn Centres
PO Box 1015
1940 EA Beverwijk
The Netherlands
Curriculum
Professor Dr. Esther Middelkoop studied chemistry at the University of Utrecht,
and obtained a PhD in biochemistry in 1989. During some years she worked as
post-doc at the biochemistry department of the University of Amsterdam, in the
Academic Medical Centre.
In 1992 she started her work on skin replacement materials at the department of
dermatology of the AMC. In 1996 she continued this work at the Burn Centre of the
Red Cross Hospital in Beverwijk and with the Dutch Burns Foundation. She initiated
several studies in the field of skin substitutes, wound healing and scar treatment,
which lead a.o. to some 10 PhD-theses.
Since January 2004, the research activities of the three dutch burn centers are
combined in the Association of Dutch Burn Centres. Esther Middelkoop is the
research director of the organization. Since September 2006, she is also professor
in Skin Regeneration and Wound Healing at the dept. of Plastic, Reconstructive and
Hand Surgery of the Free University Medical Centre in Amsterdam.
Internationally, Esther Middelkoop participates actively in the European Tissue
Repair Society (president 2003-2004), European Burns Association and Scar Club
(Montpellier). She regularly teaches at the International Skin College and the Scar
Academy.
Some Key Publications (not including abstracts)
1
De Vries, H.J.C., Mekkes, J.R., Middelkoop, E., Hinrichs, W.L.J., Wildevuur, Ch.R.H. and
Westerhof, W. (1993) Dermal substitutes for full thickness wounds in a one stage grafting model.
Wound Rep. Regen., 1, 244-252.
2
De Vries, H.J.C., Middelkoop, E., Van Heemstra-Hoen, M., Wildevuur, C.H.R., Westerhof, W.
(1995) Stromal cells from subcutaneous adipose tissue seeded in a native collagen/elastin dermal
substitute reduce wound contraction in full thickness skin defects. Lab. Invest. 73, 532-540.
3.
Lamme, E.N., de Vries, H.J.C., van Veen, H., Gabbiani, G., Westerhof, W., Middelkoop, E.
(1996) Extracellular matrix characterization during healing of full thickness wounds treated with a
collagen/elastin dermal substitute shows improved skin regeneration in pigs. J. Histochem. Cytochem.
44, 1311-1322.
4
Loots, M.A.M., Lamme, E.N. Mekkes, J.R., Bos, J.D., Middelkoop, E. (1999) Cultured
fibroblasts from chronic diabetic wounds on the lower extremity (non-insulin dependent diabetes
mellitus) show disturbed proliferation. Arch. Dermatol. Res. 291, 93-99.
5
Lamme, E.N., Van Leeuwen, R.T.J., Brandsma, K., Van Marle, J., Middelkoop, E. (2000)
Higher numbers of autologous fibroblasts in an artificial dermal substitute improve tissue regeneration
and modulate scar tissue formation. J. Pathol. 190, 595-603.
6
Van Zuijlen, P.P.M., Van Trier, A.J.M., Vloemans, A.F.P.M., Groenevelt, F., Kreis, R.W.,
Middelkoop, E. (2000) Graft survival and effectiveness of dermal substitution in burns and
reconstructive surgery in a one-stage grafting model. Plast. Rec. Surg. 106, 615-623.
7
Vloemans, A.F.P.M., Soesman, A.M., Kreis, R.W., Middelkoop, E. (2001) A newly developed
hydrofibre dressing in the treatment of partial thickness burns. Burns, 27, 167-173.
8
Van den Bogaerdt, A.J., van Zuijlen, P.P.M., van Galen, M.J.M., Lamme, E.N., Middelkoop, E.
(2002) The suitability of cells from different tissues to be used in tissue engineered skin substitutes.
Arch. Dermatol. Res. 294, 135-142.
9
Loots, M.A.M., Kenter, S.B., Au, F.L., van Galen, W.J.M., Middelkoop, E., Bos, J.D., Mekkes,
J.R. (2002) Fibroblasts derived from chronic diabetic ulcers differ in their response to stimulation with
EGF, IGF-I, bFGF and PDGF-AB compared to controls. Eur. J. Cell Biol. 81(3), 153-160.
10
Vloemans AF, Soesman AM, Suijker M, Kreis RW, Middelkoop E. (2003) A randomised
clinical trial comparing a hydrocolloid-derived dressing and glycerol preserved allograft skin in the
management of partial thickness burns. Burns. Nov;29(7):702-10.
11
Middelkoop E, van den Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MM.
(2004) Porcine wound models for skin substitution and burn treatment. Biomaterials. Apr;25(9):155967.
12
Van den Bogaerdt AJ, Ulrich MWW, van Galen MJM, Reijnen L, Verkerk M, Pieper J, Lamme
EN, Middelkoop E. Upside-down transfer of porcine keratinocytes from a porous, synthetic dressing to
experimental full-thickness wounds. Wound Repair Regen. 2004 Mar-Apr;12(2):225-34.
13
Draaijers LJ, Tempelman FSH, Botman YAM, Middelkoop E, Kreis RW, Tuinebreijer WE, van
Zuijlen PPM. The Patient and Observer Scar Assessment Scale (POSAS): a reliable and feasible tool
for scar evaluation. Plast. Rec. Surg. Plas Recon Surg 2004;113(7):1960-65
Discussed by Thomas A. Mustoe, Plast Recon Surg 2004;113:1966-1967
14
Duc le Q, Breetveld M, Middelkoop E, Scheper RJ, Ulrich MMW, Gibbs S. A cytotoxic analysis
of antiseptic medication on skin substitutes and autograft. British J of Derm 2007; 157: 33-40
15
Coolen NA, Bogaerdt van den AJ, Middelkoop E, Ulrich MMW. Development of an in vitro burn
wound model. Wound Repair and Regeneration 2008 Jul-Aug; 16(4): 559-567
16.
Bloemen MCT, Veer van der WM, Ulrich MMW, Zuijlen van PPM, Niessen FB, Middelkoop E.
Prevention and curative management of hypertrophic scar formation. Burns 2009 June; 35(4): 463475; Epub 2008; October 23
17.
Veer van der WM, Bloemen MCT, Ulrich MMW, Molema G, Zuijlen van PPM, Middelkoop E,
Niessen FB. Potential cellular and molecular causes of hypertrophic scar formation. Burns 2009
February; 35(1): 15-29; Epub 2008; October 23
18.
Bogaerdt van den AJ, Veen van der VC, Zuijlen van PPM, Reijnen L, Verkerk M, Bank RA,
Middelkoop E, Ulrich MMW. Collagen crosslinking by adipose-derived mesenchymal stromal cells and
scar-derived mesenchymal cells; are mesenchymal stromal cells involved in scar formation? Wound
Repair and Regeneration: 2009; 17 (4): 548-558.
19.
Veen van der VC, Wal van der MBA, Leeuwen van MCE, Ulrich MMW, Middelkoop E
Biological background of dermal substitutes. Burns; 2010 May;36(3):305-21.
20.
Bloemen MCT, van Leeuwen MCE, van Vucht NE, van Zuijlen PPM, Middelkoop E. Dermal
Substitution in Acute Burns and Reconstructive Surgery: A 12-Year Follow-Up. Plast Rec Surg. 2010
May;125(5):1450-9.
21.
Coolen NA, Schouten KC, Boekema BK, Middelkoop E, Ulrich MM Wound healing in a fetal,
adult, and scar tissue model: a comparative study. Wound Repair Regen. 2010 May 1;18(3):291-301.
22.
van der Wal MB, van Zuijlen PP, van de Ven P, Middelkoop E. Topical silicone gel versus
placebo in promoting the maturation of burn scars: a randomized controlled trial. Plast Reconstr Surg.
2010 Aug;126(2):524-31.
23.
Verhaegen PD, van Trier AJ, Jongen SJ, Vlig M, Nieuwenhuis MK, Middelkoop E, van Zuijlen
PP. Efficacy of skin stretching for burn scar excision: a multicenter randomized controlled trial. Plast
Reconstr Surg. 2011 May;127(5):1958-66.
24.
van der Wal MB, Tuinebreijer WE, Bloemen MC, Verhaegen PD, Middelkoop E, van Zuijlen
PP. Rasch analysis of the Patient and Observer Scar Assessment Scale (POSAS) in burn scars. Qual
Life Res. 2011 May 20. [Epub ahead of print].