Title: Efficacy of Management Tools for Pythium Root Rot.
Protocol #: 13-009
Draft 2
p 1 of 6
Objective: Determine efficacy of new active ingredient formulations and new biopesticides for managing root,
crown and stem rots of ornamental plants caused by Pythium species.
Experimental Design:
Plot Size: Must be adequate to reflect actual use conditions.
Replicates: Minimum of 4.
Application Instructions: If plants are to be transplanted, transplant at least 5 days prior to treatment
applications so any wounds occurring during transplanting will heal and not serve as access points for
disease infection and/or not contribute to increased phytotoxicity. Drench each treatment 2-3 days prior
to inoculation with known species of Pythium. For woody plant material, reapply treatments 4 weeks
later. For phosphorus acid generators, a foliar application may be substituted for the drench application.
Applications should be made using application equipment consistent with conventional commercial
equipment. Calibrate application equipment prior to application. Read available labels before making
applications. Avoid treating plants under unusually extreme environmental conditions.
Target Species: Pythium species. Please identify target species or use a known colony for inoculation.
Protocol will vary depending on Pythium species; see treatment tables below.
Plant Hosts: Use a plant host suitable for target species, recording species and variety used.
Use Site: May be greenhouse, field container or field in-ground. Please specify in final report.
Evaluations: Record disease severity and incidence 0, 7, 14, and 28 days after application for herbaceous
plant material. If Pythium damping off is being assessed, record disease severity and incidence, 0, 3, and
7 days after application. For woody plant material, record disease severity and incidence 0, 14, 28, 42
and 56 days after initial application. Record plant height & width at initial and final evaluations only.
After rating root disease incidence and severity, collect dry weights. Record phytotoxicity at each rating
date on a scale of 0 to 10 (0 = no phytotoxicity; 10 = complete kill). If phytotoxicity is observed in
treated plants, take pictures comparing treated and untreated plant material.
Recordkeeping:
All operations, data and observations appropriate to this study should be recorded. It is helpful to review the
Ornamental Horticulture Research Report Form in advance. Keep detailed records of weather conditions
including temperature, precipitation and/or irrigation, and relative humidity with a minimum of high, low
and average daily temperatures. Other information to record includes soil-type or soil-less media,
application equipment, irrigation (type & frequency), liner size, plant height & width, and plant growth
stage at application and data collection dates. Content of reports should be sufficient to fully understand how
the experiment was conducted.
Photographs often illustrate experimental design, site conditions, and impacts of treatments very well. It is
encouraged to include a picture or two of the greenhouse, field or landscape where the experiment is sited. It
is highly encouraged that pictures illustrating treatment effects are taken if and when these impacts are
visually apparent.
If different application methods or evaluations are made, please clearly specify differences in final report
and explain reason for change.
Treatments:
See tables starting on the following page. There are two tables of A priority treatments based on whether
data has been generated on specific Pythium species.
Title: Efficacy of Management Tools for Pythium Root Rot.
Protocol #: 13-009
Draft 2
p 2 of 6
Product
Registration Status
Need Data On:
P.
aphanadermatum
P.
dissotichum
P.
irregulare
P.
ultimum
P.
vipa
Adorn
Yes – general + P.
aphanidermatum
and P. irregulare
N
Y
N
N
Y
CG100
NO
Y
Y
Y
Y
Y
DisArm
Yes - general
N
Y
N
N
Y
FenStop
Yes - general
N
Y
Y (1)
N
Y
Heritage
NO
N
Y
Y (1)
N
Y
Pageant
Yes - general
N
Y
Y
N
Y
RootShield Plus
Yes - general
N
Y
Y
Y
Y
SP2770 10WP
NO
Y
Y
Y
Y
Y
SP2771
NO
Y
Y
Y
Y
Y
Tank Mix:
acibenzolar +
Heritage
NO
Y
Y
Y
Y
Y
Treatments for P. aphanidermatum, P. irregulare, and P. ultimum:
Priority
Rates (per 100 gal
unless otherwise stated)
Reapplication
Interval
1
Cg100
38.4 fl oz
28 day
2
3
SP2770 10WP
SP2771
2.66 lb
3 fl oz
Heritage (azoxystrobin)
0.9 oz
A
B (pick 3)
Standards
RootShield Plus (BW240
WP)
6 oz
½ rate 10 to 12
weeks
7
8
9
10
Standard Control 1*
Standard Control 2*
Untreated Uninoculated
Untreated Inoculated
See below
See below
---
See below
See below
---
a
b
2 fl oz
14 days
See label for specifics
0.6 oz
28 day
Apply as a drench
d
e
Adorn
Aliette (fosetyl Al)
Disarm 480 SC
(fluoxastrobin)
FenStop (fenamidone)
Segway (cyazofamid)
14 oz
28 day
Greenhouse application
f
Subdue Maxx (mefenoxam)
21-28 days
Apply as a drench
g
Terrazole L (etridiazole)
4 to 12 weeks
Apply as a drench
4
5
6
Pageant 38 WG
(pyraclostrobin + boscalid)
12 oz
Apply as a drench
Apply as a sprench to crown of the plant and
soil delivering 200 ml of solution per 6 in
pot.
Apply BW240 72 hours before pathogen
inoculation or dip rooted cuttings at
transplant PREVENTATIVE MATERIAL
AT LIGHT TO MODERATE DISEASE
PRESSURE NOT CURATIVE
See below
See below
---
c
Standards
28 day
Special Application Instructions (Soil
Drench)
Drench. Mix cg100 thoroughly
in water and apply to soil within 10 mins
after mixing.
16 oz
1 oz for Herbaceous
2 oz for Woodies
7 fl oz
14 day
Title: Efficacy of Management Tools for Pythium Root Rot.
Product
Protocol #: 13-009
#
Draft 2
p 3 of 6
Treatments for all other Pythium species:
Product
A
1
Adorn 4FL (fluopicolide)
A
2
Cg100
A
3
A
A
4
5
B
(choose 3)
Standards
Standards
6
7
8
Rates (per 100 gal
unless otherwise stated)
Reapplication
Interval
2 fl oz
14 days
38.4 fl oz
28 day
Special Application Instructions (Soil
Drench)
Soil Drench Volume
Bedding flats: 1 pint/ sq ft
4” pots: 4 fl oz/ pot
6” pots: 6 fl oz/ pot
See label for other pot sizes
Limit to 2 applications
Drench. Mix cg100 thoroughly
in water and apply to soil within 10 mins after
mixing.
28 day
Apply as a drench
2.66 lb
3 fl oz
1.8 oz
14 oz
28 day
28 day
16 oz
14 day
RootShield (BW240 WP)
6 oz
½ rate 10 to 12
weeks
See below
See below
---
See below
See below
---
Apply as a drench
Greenhouse application
Apply as a sprench to crown of the plant and
soil delivering 200 ml of solution per 6 in pot.
Apply BW240 72 hours before pathogen
inoculation or dip rooted cuttings at transplant
PREVENTATIVE MATERIAL AT LIGHT
TO MODERATE DISEASE PRESSURE NOT
CURATIVE
See below
See below
---
1 lb (200 ppm)
4-5 days
Apply as preventive or at first signs of watersoaked lesions
21-28 days
Apply as a drench
4 to 12 weeks
Apply as a drench
9
10
11
12
Standard Control 1*
Standard Control 2*
Untreated Uninoculated
Untreated Inoculated
a
AgriMycin
b
c
Aliette (fosetyl Al)
Segway (cyazofamid)
d
Subdue Maxx (mefenoxam)
e
Terrazole L (etridiazole)
1 oz for Herbaceous
2 oz for Woodies
7 fl oz
Draft 2
0.6 oz
p 4 of 6
Disarm 480 SC
(fluoxastrobin)
SP2770 10WP
SP2771
Heritage (azoxystrobin)
FenStop (fenamidone)
Pageant 38 WG
(pyraclostrobin + boscalid)
Title: Efficacy of Management Tools for Pythium Root Rot.
#
Protocol #: 13-009
Priority
Priority
Product
Heritage + Subdue
B
Insignia (Pyraclostrobin)
Magellan (mono- and dibasic
sodium, potassium and
ammonium phosphites)
Remedier (Trichoderma
harzianum & viride)
Rotation: BW240 / Phosphorous
acid generator
Vital (potassium phosphate)
B
B
B
0.5 oz + 1 oz
21-28 day
interval
Special Application Instructions
Apply as a drench
6 oz/100 gal
Use label rate
Title: Efficacy of Management Tools for Pythium Root Rot.
B
Reapplication
Interval
Protocol #: 13-009
B
C
Agri-Fos (dipotassium
phosphonate + dipotassium
phosphate)
Alude (potassium phosphate)
Captan
B
Rates
Draft 2
p 5 of 6
Title: Efficacy of Management Tools for Pythium Root Rot
Protocol #: 13-009
Draft 2
p 6 of 6
Sources for Products:
Product
Acibenzolar
Adorn
AgriFos
Agrimycin
Aliette
Alude
BW240 WP
Cg100
DisArm
FenStop
Heritage
Insignia
Magellan
Pageant
Remedier
Segway
SP2770
10WP
SP2771
SubdueMaxx
Terrazole L
Vital
Contact
Syngenta, Steve Cosky
Valent, Joe Chamberlin
AgBio, Jan Meneley
Nufarm, Tom Kroll
Bayer, Don Myers
Cleary Chemical, Rick Fletcher
BioWorks, Randy Martin
Summerdale, Bob Coleman
OHP, Jeff Dobbs
OHP, Jeff Dobbs
Syngenta, Steve Cosky
BASF, Kathie Kalmowitz
Nufarm, Tom Kroll
BASF, Kathie Kalmowitz
IsaGro, Dennis Krass
FMC, Bobby Walls
Phone
336-632-7148
770-985-0303
303-469-9221
715-386-1133
919-549-2529
732-329-8399
800-877-9443 x 307
608-826-6667
770-992-0121
770-992-0121
336-632-7148
919-270-4592
715-386-1133
919-270-4592
919-321-5201
919-735-3862
Email
steve.cosky@syngenta.com
jcham@valent.com
agbio@agbio-inc.com
tom.kroll@us.nufarm.com
don.myers@bayercropscience.com
rick.fletcher@clearychemical.com
rmartin@bioworksinc.com
colema95@msu.edu
jdobbs@OHP.com
jdobbs@OHP.com
steve.cosky@syngenta.com
kathie.kalmowitz@basf.com
tom.kroll@us.nufarm.com
kathie.kalmowitz@basf.com
dkrass@isagro-usa.com
Bobby.Walls@fmc.com
SePRO, Jessica Koczan
252-391-8382
jessicak@sepro.com
SePRO, Jessica Koczan
Syngenta, Steve Cosky
OHP, Jeff Dobbs
Luxembourg Industries, Vince
Morton
252-391-8382
336-632-7148
770-992-0121
jessicak@sepro.com
steve.cosky@syngenta.com
jdobbs@OHP.com
336-286-9714
mortv@aol.com
Reports:
Reports are due 60 days after last data collection date.
Submit reports electronically on the standard IR-4 Ornamental Horticulture Research Report Form. The
basic report can be sent in MS Word, the recordkeeping information as pdf or other electronic
documents, the raw data in MS Excel or other suitable program such as ARM, and photographs can be
submitted as picture embedded in the report or as separate jpg, bmp, or tiff files.
Please direct questions to:
Cristi Palmer, IR-4 HQ, Rutgers University, 500 College Road East, Suite 201W, Princeton, NJ 08540,
Phone 732-932-9575 x4629, palmer@aesop.rutgers.edu
OR
Ely Vea, 308 Aston Forest Lane, Crownsville, MD 21032, Phone & FAX#: 410-923-4880, E-mail:
evvea@comcast.net.
Draft Date: 10/23/2012
Revised By: CLP