CURRICULUM
VITAE
EUROPEAN FORMAT
PERSONAL INFORMATION
Name
Address
MYRIAM ALCALAY
VIA ADAMELLO 16, 20139, MILANO, ITALIA
Telephone
02 94375203
Fax
02 94375990
E-mail
myriam.alcalay@unimi.it
myriam.alcalay@ieo.eu
Italian, Chilean
Date and Place of Birth MAY 23 1961, SANTIAGO, CHILE
Citizenship
WORK EXPERIENCE
• Dates (from – to)
• Name and address of employer
• Type of business or sector
•Position held
• Main activities and responsibilities
• Dates (from – to)
• Name and address of employer
• Type of business or sector
•Position held
• Main activities and responsibilities
• Dates (from – to)
• Name and address of employer
• Type of business or sector
•Position held
2006 - present
Università degli Studi di Milano
Dipartimento di Scienze della Salute (DISS)
Via A. Di Rudinì 8, 20142, Milano
University – School of Medicine
Associate Professor – General Pathology (Mechanisms of Disease)
Teaching
2005 - present
Istituto Europeo di Oncologia, Experimental Oncology
c/o IFOM-IEO Campus, Via Adamello 16, 20139, Milan, Italy
Research Laboratory
Group Leader – Functional Genomics
Directs her own research group. The main projects include:
 Analysis of transcriptional networks underlying normal hematopoietic differentiation
and their subversion in the pathogenesis of acute myeloid leukemias (AML).
 Identification of somatic mutations with prognostic/predictive relevance.
See http://www.ifom-ieo-campus.it/groups/alcalay for a more detailed description.
2007 - 2011
Cogentech – Consortium for Genomic Technologies
IFOM-IEO Campus, Via Adamello 16, 20139, Milan
Core facilities for biomedical research
Scientific Director
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• Main activities and responsibilities
Coordination, organization and scientific direction of all core facilities for the IFOM-IEO Campus,
including:
 RT-PCR Facility
 Sequencing Facility
 Microarray Unit
 Molecular Pathology Unit
 Imaging Unit
 Protein Biochemistry Unit
 Proteomics Unit
 Crystallization Unit
 Mouse Genetics Facility
 Transgenic Facility
 Zebrafish Facility
Cogentech included commercial services available for outside users.
• Dates (from – to)
• Name and address of employer
2003 - 2007
Cogentech – Consortium for Genomic Technologies
IFOM-IEO Campus, Via Adamello 16, 20139, Milan, Italy
Microarray Unit
Director
Coordinated and directed the activity of the microarray facility that serves the IFOM and IEO
research groups. The facility performed:
 Gene expression profiling experiments using Affymetrix technology (for both internal
and external users - see http://services.ifom-ieo-campus.it/).
 SNP analysis using Affymetrix technology,
 Exclusive distributor of Nimblegen technology for Italy
Dr. Alcalay was personally responsible for choosing experimental procedures, and protocols
used by the facility. She interacted directly with the users in counseling for optimal experimental
design, and in data analysis and elaboration.
• Type of business or sector
•Position held
• Main activities and responsibilities
• Dates (from – to)
• Name and address of employer
• Type of business or sector
• Position held
• Main activities and responsibilities
• Dates (from – to)
• Name and address of employer
• Type of business or sector
•Position held
1995 - 2005
Department of Experimental Oncology
European Institute of Oncology
Via Ripamonti 435, 20141, Milan, Italy
Research Laboratory
Senior staff scientist
Supervision of selected trainees and individual research projects including:
 Identification of a functional interaction between PML and pRB proteins
 Analysis of gene expression patterns in AML using microarray technology.
 Analysis of the molecular basis of Retinoic Acid therapy.
 Analysis of NPM mutations in AML with normal karyotype, and of the associated
expression profile.
1987 - 1995
Molecular Biology Lab – Istituto di Clinica Medica I
University of Perugia
Policlinico Monteluce, 06100, Perugia, Italy
Molecular Biology Laboratory
Research Fellow
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• Main activities and responsibilities
Independent research projects, including:
 Characterization of the myeloid specific human c-fes oncogene.
 Cloning of the t(15;17) translocation of acute promyelocytic leukemia (APL).
 Characterization of the PML/RAR fusion gene.
 Cloning and characterization of the PML gene
 Analysis of expression of the RAR/PML fusion gene in APL patients
 Molecular analysis of APLs lacking the t(15;17) translocation
and collaborative research projects, including:
 Characterization of the PML/RAR fusion transcript.
 Development of PCR-based assays for diagnosis and evaluation of disease in APL
 Analysis of the effects of PML/RAR on differentiation and survival of myeloid
precursors
• Dates (from – to)
• Name and address of employer
1986 - 1987
Istituto Internazionale di Genetica e Biofisica (IIGB), CNR
Viale Marconi 10, 80125, Naples, Italy
Human Genetics & Molecular Biology Laboratory
Research Fellow
Basic training in Molecular Biology techniques. Research projects included:
 Characterization of a group of genes located in the long arm of the X chromosome.
 Molecular characterization of the G6PD gene mutations in a group of Sardinian
patients with G6PD deficiency.
• Type of business or sector
• Position held
• Main activities and responsibilities
• Dates (from – to)
• Name and address of employer
• Type of business or sector
• Occupation or position held
• Main activities and responsibilities
EDUCATION
1985 - 1986
Istituto di Clinica Medica I
University of Perugia
Policlinico Monteluce, 06100, Perugia, Italy
Clinical Hematology
Medical Intern
Clinical training in the filed of hematology/oncology.
• Dates (from – to)
• Name and type of organisation
providing education and training
• Principal subjects covered
• Title of qualification awarded
1986 - 1990
University of Perugia
• Dates (from – to)
• Name and type of organisation
providing education and training
• Principal subjects covered
• Title of qualification awarded
1979 - 1985
University of Perugia
• Dates (from – to)
• Name of School
• Principal subjects covered
• Title of qualification awarded
• Dates (from – to)
Hematology
PhD
Medical School
Medical Degree – 110/110 cum laude
1978 - 1979
New School, Rome, Italy
Mathematics, Biology, Physics
U.K. General Certificate of Education (GCE) of the University of London, Advanced Level
degree.
1977
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• Name of School
• Principal subjects covered
• Title of qualification awarded
TEACHING EXPERIENCE
New School, Rome, Italy
Mathematics, Biology, Physics, History, English Language, Spanish, French, Italian, English
Literature
U.K. General Certificate of Education (GCE) of the Universities of London or Cambridge,
Ordinary Level degree
• Dates (from – to)
• Name and type of teaching activity
2006 - present
Associate Professor of General Pathology (Mechanisms of Disease), School of Medicine,
Università degli Studi di Milano, Milan.
• Dates (from – to)
• Name and type of teaching activity
2005 - present
Faculty member of the European School of Molecular Medicine (SEMM) PhD programme in
Molecular Oncology, Organizer of the Genomics and Proteomics course.
• Dates (from – to)
• Name and type of teaching activity
2005-2007
Teaching assignment in "Gene Expression". Biotechnology Course, University “Vita Salute, San
Raffaele”, Milan.
• Dates (from – to)
• Name and type of teaching activity
2003-2004
Teaching assignment - Master in “Molecular Immunopathology”, Department of Biotechnology
and Bioscience, University of Milan “Bicocca”.
• Dates (from – to)
• Name and type of teaching activity
1997
ICGEB International Course "Molecular Techniques of Genome Mapping and Screening",
Santiago, Chile.
• Dates (from – to)
• Name and type of teaching activity
1992-1996
Teaching assignment in the Internal Medicine School, University of Perugia.
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ACTIVITY AS PEER REVIEWER
Journals:
Funding agencies:
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American Journal of Hematology
BBA – Molecular Cell Research
Blood
BMC Bioinformatics
BMC Genomics
Cell Death and Differentiation
Clinical Chemistry
ecancermedicalscience
Haematologica
International Journal of Biochemistry and Cell Biology
Leukemia
Molecular and Cellular Biology
Molecular and Cellular Endocrinology
Molecular Cancer
Nature
PLoS Genetics
PLoS ONE
Redox Reports
Associazione Italiana per la Ricerca sul Cancro (AIRC)
Dutch Cancer Society (KUN)
European Research Council (ERC)
Fondazione Umberto Veronesi (FUV)
French National Cancer Institute (INC)
Italian Ministry of University and Research (MIUR)
Swiss National Science Foundation (SNF)
United States – Israel Binational Science Foundation (BSF)
Wellcome Trust
LANGUAGES
MOTHER TONGUE
SPANISH
OTHER LANGUAGES
• Reading skills
• Writing skills
• Verbal skills
ENGLISH
excellent
excellent
excellent
• Reading skills
• Writing skills
• Verbal skills
ITALIAN
excellent
excellent
excellent
• Reading skills
• Writing skills
• Verbal skills
SERBO-CROATIAN
basic
basic
good
• Reading skills
• Writing skills
• Verbal skills
FRENCH
basic
basic
basic
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SUMMARY OF RESEARCH INTERESTS
Prof. Alcalay has been actively involved in the study of the molecular defects underlying acute myeloid leukemia (AML) since
1987. Major achievements in this area include: cloning of the 15;17 translocation characteristic of acute promyelocytic
leukemia, functional analysis of the PML/RAR fusion protein, analysis of transcriptional regulation associated to expression of
the leukemogenic fusion proteins PML/RAR, PLZF/RAR and AML1/ETO, identification of mutations in the nucleophosmin
(NPM) gene in AML patients with normal karyotype, identification of a specific expression profile associated to AML with NPM
mutations, analysis of AML1/ETO genomic binding pattern and identification of the cell-cycle inhibitor p21 as a crucial player
in the maintenance of self-renewal of leukemia stem cells. She has solid experience in the use of functional and genomic
approaches, and has focused much of her interest in the past decade on exploiting an integrated genomic approach to study
the molecular basis of acute leukemias.
Her current interests include analyses of transcriptional networks underlying normal hematopoietic differentiation and their
subversion in the pathogenesis of leukemogenesis through the integration of data deriving from mRNA and miRNA
expression profiling, chromatin immunoprecipitation coupled to deep sequencing (ChIP-seq), identification of histone
modifications and proteomic analysis of transcriptional complexes containing leukemia-associated oncoproteins. Other
projects addressing the discovery of somatic mutations with prognostic/predictive relevance in AML, and oncogene-induced
activation of stem-cell signalling pathways in leukemia are ongoing in her laboratory.
Prof. Alcalay has always supported her independent research through funding obtained as Unit Leader from the Italian
Association for Cancer Research (AIRC), the Ministry of University and Research (MIUR), the Italian Ministry of Health, the
Cariplo Foundation and the Umberto Veronesi Foundation (FUV).
May 2014
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COMPLETE LIST OF PUBLICATIONS
ORIGINAL ARTICLES
1. L. Riva, C. Ronchini, M. Bodini, F. Lo-Coco, S. Lavorgna, T. Ottone, G. Martinelli, I. Iacobucci, C. Tarella, A. Cignetti, S.
Volorio, L. Bernard, A. Russo, G.E. Melloni, L. Luzi, M. Alcalay, G.I. Dellino, P.G. Pelicci: Acute promyelocytic leukemias
share cooperative mutations with other myeloid-leukemia subgroups. Blood Cancer J. 2013 Sep 13;3:e147.
2. Gambino V, De Michele G, Venezia O, Migliaccio P, Dall'olio V, Bernard L, Minardi SP, Fazia MA, Bartoli D, Servillo G,
Alcalay M, Luzi L, Giorgio M, Scrable H, Pelicci PG, Migliaccio E.: Oxidative stress activates a specific p53
transcriptional response that regulates cellular senescence and aging. Aging Cell. 2013 Feb 28. doi: 10.1111/acel.12060.
[Epub ahead of print]
3. E. Pelosi, G. Castelli, I. Martin-Padura, V. Bordoni, S. Santoro, A. Conigliaro, A.M. Cerio, M De Santis Puzzonia, P.
Marighetti, M. Biffoni, T. Alonzi, L. Amicone, M. Alcalay, F. Bertolini, U. Testa, M. Tripodi.: Human haemato-endothelial
precursors: cord blood CD34+ cells produce haemogenic endothelium. PLoS One, 2012;7(12):e51109. doi:
10.1371/journal.pone.0051109. Epub 2012 Dec 4.
4. C. Giampietro, A. Taddei, M. Corada, G.M. Sarra-Ferraris, M. Alcalay, U. Cavallaro, F. Orsenigo, M.G. Lampugnani, E.
Dejana: Overlapping and divergent signalling pathways of N- and VE-cadherin in endothelial cells. Blood, 2012, Mar
1;119(9):2159-70.
5. S. Licciulli, C. Luise, G. Scafetta, M. Capra, G. Giardina, P. Nuciforo, S. Bosari, G. Viale, C. Tonelli, L. Lanfrancone and
M. Alcalay: Pirin inhibits cellular senescence in melanocytic cells. American Journal of Pathology, 2011, 178(5):2397406.
6. M. Fanelli, S. Amatori, I. Barozzi, M. Soncini, R. Dal Zuffo, G. Bucci, M. Capra, M. Quarto, G. I. Dellino, C. Mercurio, M.
Alcalay, G. Viale, P. G. Pelicci, S. Minucci: PAT-ChIP-Seq: a tool for the epigenetic profiling of formaldehyde fixed and
paraffin embedded (FFPE) pathology samples. P Natl Acad Sci Usa, 2010, 14;107(50):21535-40.
7. S. Licciulli, C. Luise, A. Zanardi, L. Giorgetti, G. Viale, L. Lanfrancone, R. Carbone and M. Alcalay: Pirin delocalization in
melanoma progression identified by high content immuno-detection based approaches. BMC Cell biology, 2010, 11:5.
8. S. Licciulli, V. Cambiaghi, G. Scafetta, A. M. Gruszka and M. Alcalay: Pirin downregulation is a feature of AML and leads
to impairment of terminal myeloid differentiation. Leukemia, 2010 Feb;24(2):429-37.
9. N. Meani, F. Pezzimenti, G. Deflorian, M. Mione, M. Alcalay: The tumor suppressor PRDM5 regulates Wnt signaling at
early stages of zebrafish development. PLoS ONE, 2009, 4(1):e4273.
10. A. Viale, F. De Franco, A. Orleth, V. Cambiaghi, V. Giuliani, D. Bossi, C. Ronchini, S. Ronzoni, I. Muradore, S.
Monestiroli, A. Gobbi, M. Alcalay, S. Minucci and P. G. Pelicci: Cell-cycle restriction limits DNA damage and maintains
self-renewal of leukaemia stem cells. Nature. 2009, Jan. 1, 457, 51-56.
11. A. Gardini, M. Cesaroni, L. Luzi, S. P. Minardi, E. Venturini, P. G. Pelicci and M. Alcalay: AML1/ETO oncoprotein is
directed to AML1 binding regions and draws E-protein HEB onto its targets. PLoS Genetics, 2008, Nov;4(11).
12. I. Fumasoni, N. Meani, D. Rambaldi, G. Scafetta, M. Alcalay, and F. D. Ciccarelli: Family expansion and gene
rearrangements contributed to the functional specialization of PRDM genes in vertebrates. BMC Evol. Biol., 2007; Oct 4;
7: 187-198.
13. S. Senese, K. Zaragoza, S. Minardi, I. Muradore, S. Ronzoni, A. Passafaro, L. Bernard, G.F. Draetta, M. Alcalay, C.
Seiser, S. Chiocca: A role for histone deacetylase 1 in human tumor cell proliferation. Mol Cell Biol. 2007 Jul;27(13):478495.
14. B. Falini, M. P. Martelli, N. Bolli, R. Bonasso, E. Ghia, M. T. Pallotta, D. Diverio, I. Nicoletti, R. Pacini, A. Tabarrini, B.
Verducci Galletti, R. Mannucci, G. Roti, R. Rosati, G. Specchia, A. Liso, E. Tiacci, M. Alcalay, L. Luzi, S. Volorio, L.
Bernard, A. Guarini, S. Amadori, F. Mandelli, F. Pane, F. Lo Coco, G. Saglio, P. G. Pelicci, M. F. Martelli, and C. Mecucci:
Immunohistochemistry predicts nucleophosmin (NPM) mutations in acute myeloid leukemia. Blood, 2006 Sep
15;108(6):1999-2005.
15. A.R. Mariano, E. Colombo, L. Luzi, P. Martinelli, S. Volorio, L. Bernard, N. Meani, R. Bergomas, M. Alcalay and P.G.
Pelicci: Cytoplasmic localization of NPM in myeloid leukemias is dictated by gain-of-function mutations that create a
functional nuclear export signal. Oncogene, 2006, Jul 20;25(31):4376-80
16. M. S. Carro, F. M. Spiga, M. Quarto, V. Di Ninni, S.Volorio, M. Alcalay and H. Müller: DEK expression is controlled by
E2F and deregulated in diverse tumor types. Cell Cycle, 2006, Jun;5(11):1202-7.
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17. M. Ceribelli, M. Alcalay, MA Vigano, and R. Mantovani: Repression of new p53 targets revealed by ChIP on chip
experiments. Cell Cycle. 2006 May;5(10):1102-10.
18. E. Colombo, P. Martinelli, R. Zamponi, D. C. Shing, P. Bonetti, L. Luzi,, S. Volorio, L. Bernard, G. Pruneri, M. Alcalay,
and P. G. Pelicci: Delocalization and Destabilization of the Arf Tumor Suppressor by the Leukemia-Associated NPM
Mutant. Cancer Res. 2006 Mar, 66, 3044-3050.
19. Doumont G, Martoriati A, Beekman C, Bogaerts S, Mee PJ, Bureau F, Colombo E, Alcalay M, Bellefroid E, Marchesi F,
Scanziani E, Pelicci PG, Marine JC.: G1 checkpoint failure and increased tumor susceptibility in mice lacking the novel
p53 target Ptprv. EMBO J. 2005 Sep 7;24(17):3093-103.
20. M. Alcalay, E. Tiacci, R. Bergomas, B. Bigerna, E. Venturini, S. P. Minardi, N. Meani, D. Diverio, L. Bernard, L. Tizzoni,
S. Volorio, L. Luzi, E. Colombo, F. Lo Coco, C. Mecucci, B. Falini and P. G. Pelicci: Acute Myeloid Leukemia bearing
cytoplasmic nucleophosmin (NPMc+ AML) shows a distinct gene expression profile characterized by up-regulation of
genes involved in stem cell maintenance. Blood. 2005 Aug 1;106(3):899-902..
21. N. Meani, S. P. Minardi, Silvia Licciulli, V. Gelmetti, F. Lo Coco, C. Nervi, P. G. Pelicci, H. Müller, and M. Alcalay:
Molecular Signature of Retinoic Acid Treatment in Acute Promyleocytic Leukemia. Oncogene, 2005 May 5;24(20):335868.
22. F. Fazi, L. Travaglini, D. Carotti, F. Palitti, D. Diverio, M. Alcalay, S. McNamara, W.H. Miller Jr, F. Lo Coco, P.G. Pelicci
and C. Nervi: Retinoic acid targets DNA-methyltransferases and histone deacetylases during APL blast differentiation in
vitro and in vivo. Oncogene, 2005 Mar 10;24(11):1820-30.
23. B. Falini, C. Mecucci, E. Tiacci, M. Alcalay, R. Rosati, L. Pasqualucci, R. La Starza, D. Diverio, E. Colombo, A. Santucci,
B. Bigerna, R. Pacini, A. Pucciarini, A. Liso, M. Vignetti, P. Fazi, N. Meani, V. Pettirossi, G. Saglio, F. Mandelli, F. LoCoco, P.G. Pelicci, and M. F. Martelli, for the GIMEMA Study Group: Cytoplasmic Nucleophosmin in Acute Myelogenous
Leukemia with a Normal Karyotype. New Engl J Med 2005;352:254-266.
24. A. Martoriati, G. Doumont, M. Alcalay, E. Bellefroid, P. G. Pelicci & J.C. Marine: DAPK1, an activator of a p19ARF-p53
mediated apoptotic checkpoint, is a transcription target of p53. Oncogene, 2005 Feb 17;24(8):1461-6.
25. G. Finocchiaro, P. Parise, S.P. Minardi, M. Alcalay, and H. Müller: GenePicker: Replicate analysis of Affymetrix gene
expression microarrays. Bioinformatics, 2004 Dec 12;20(18):3670-2.
26. M. Masseroli, A. Stella, N. Meani, M. Alcalay, and F. Pinciroli: MyWEST: a new personal Web Extraction Software Tool
for effective mining of web-interfaced biomolecular databanks. Bioinformatics, 2004 Dec 12;20(18):3326-35.
27. A. Guffanti, G. Finocchiaro, J.F. Reid, L. Luzi, M. Alcalay, S. Confalonieri and H. Müller: Automated DNA chip annotation
tables at IFOM: the importance of sequence databases synchronization and cross-references. Appl Bioinformatics, 2003:
2(4)245-249
28. M. Alcalay, N. Meani, V. Gelmetti, A. Fantozzi, M. Fagioli, A. Orleth, D. Riganelli, C. Sebastiani, E. Cappelli, C.Casciari,
M.T. Sciurpi, A.R. Mariano, S.P. Minardi, L. Luzi, H. Muller, P.P. Di Fiore, G. Frosina and P.G. Pelicci: Acute Myeloid
Leukemia fusion proteins deregulate genes involved in stem cell maintenance and DNA repair. J Clin Invest, Dec 2003;
112 (11): 1751-1762.
29. M. Masseroli, P. Cerveri, P.G. Pelicci, and M. Alcalay: GAAS: Gene Array Analyzer Software for management, analysis
and visualization of gene expression data. Bioinformatics, 2003 Apr 12;19(6):774-775.
30. C. Labbaye, M. Valtieri, F. Grignani, R. Puglisi, L. Luchetti, B. Masella, M. Alcalay, U. Testa, and C. Peschle: Expression
and role of PML gene in normal adult hematopoiesis: functional interaction between PML and Rb proteins in
erythropoiesis. Oncogene, 1999 Jun 10;18(23):3529-40.
31. M. Ruthardt, A. Orleth, L. Tomassoni, E. Puccetti, D. Riganelli, M. Alcalay, R. Mannucci, I. Nicoletti, F. Grignani, M.
Fagioli, and P.G. Pelicci: The acute promyelocytic leukaemia specific PML and PLZF proteins localize to adjacent and
functionally distinct nuclear bodies. Oncogene, 1998 Apr 16;16(15):1945-53
32. M. Alcalay, L. Tomassoni, E. Colombo, S. Stoldt, Fr. Grignani, M. Fagioli, L. Szekely, K. Helin, and P.G. Pelicci:The
promyelocytic leukemia gene product (PML) functionally interacts with the retinoblastoma protein. Mol Cell Biol 1998
Feb;18(2):1084-93
33. M. Fagioli, M. Alcalay, L. Tomassoni, P.F. Ferrucci, A. Mencarelli, D. Riganelli, Fr. Grignani, T. Pozzan, I. Nicoletti, F.
Grignani, P.G. Pelicci: Cooperation between the RING+B1-B2 and coiled-coil domains of PML is necessary for its effects
on cell survival. Oncogene, 1998 Jun 4;16(22):2905-13.
34. D. Brown, S. Kogan, E. Lagasse, I. Weissman, M. Alcalay, P.G. Pelicci, S. Atwater, J.M. Bishop: A PML/RARα transgene
initiates murine acute promyelocytic leukemia. P Natl Acad Sci Usa, 94, 2551-2556, 1997.
35. G. Meroni, A. Reymond, M. Alcalay, G. Borsani, A. Tanigami, R. Tonlorenzi, C. Lo Nigro, S. Messali, M. Zollo, D.H.
Ledbetter, R. Brent, A. Ballabio, R. Carrozzo: Rox: a novel bHLHZip protein expressed in quiescent cells that
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36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46.
47.
48.
49.
50.
51.
52.
53.
heterodimerizes with Max, binds a non-canonical “E-box”, and acts as a transcriptional repressor. EMBO J, 16 (10), 28922906, 1997.
Fr. Grignani, U. Testa, D. Rogaia, P.F. Ferrucci, P. Samoggia, A. Pinto, D. Aldinucci, V. Gelmetti, M. Fagioli, M. Alcalay,
F. Grignani, I. Nicoletti, C. Peschle, P.G. Pelicci: Effects on differentiation by the promyeloctitic leukaemia PML/RARα
protein depend on the fusion of the PML protein-dimerization and RARa DNA binding domains. EMBO J, 15 (18),
p.4949-4958, 1996.
M. Lafage-Pochitaloff, M. Alcalay, V. Brunel, L. Longo, D. Sainty, J. Simonetti, F. Birg, and P.G. Pelicci: Acute
promyelocytic leukemia cases with non- reciprocal PML/RARα or RARα/PML fusion genes. Blood, 85: 1169-1174, 1994.
U. Testa, Fr. Grignani, T. Barberi, M. Fagioli, R. Masciulli, P.F. Ferrucci, D. Seripa, M. Alcalay, P. G. Pelicci, C. Peschle:
Impaired differentiation of U937 cell mutants expressing the PML/RARα fusion protein is unblocked by retinoic acid.
Cancer Res, 1994, 54:4508-4515.
Fr. Grignani, P.F. Ferrucci, U. Testa, G. Talamo, M. Fagioli, M. Alcalay, A. Mencarelli, F. Grignani, C. Peschle, I.
Nicoletti, P.G. Pelicci: The acute promyeloctitic leukaemia specific PML/RARα fusion protein inhibits differentiation and
promotes survival of myeloid precursor cells. Cell, 74, 423-431, 1993.
F. Lo Coco, D. Diverio, F. D'Adamo, G. Avvisati, G. Alimena, M. Nanni, M. Alcalay, P.P. Pandolfi and P.G. Pelicci:
RARα/PML rearrangments in acute promyelocytic leukemias apparently lacking the t(15;17) translocation. Eur J
Haematol 48, 173-176, 1992.
A. Biondi, A. Rambaldi, P.P. Pandolfi, V. Rossi, G. Giudici, M. Alcalay, F. Lo Coco, D. Diverio, E.M. Pogliani, E.M. Lanzi,
G. Masera, T. Barbui and P.G. Pelicci: Molecular monitoring of the PML/RARα fusion gene in acute promyelocytic
leukemia by the polymerase chain reaction. Blood, 80, 492-497, 1992.
M. Alcalay, D. Zangrilli, M. Fagioli, P.P. Pandolfi, A. Mencarelli, F. Lo Coco, A. Biondi and P.G. Pelicci: Expression
pattern of the RARα/PML fusion gene in acute promyelocytic leukemia. P Natl Acad Sci Usa, 89, 4840-4844, 1992.
M. Fagioli, M. Alcalay, P.P. Pandolfi, L. Venturini, A. Mencarelli, A. Simeone, D. Acampora, F. Grignani and P.G. Pelicci:
Identification of various PML gene isoforms and characterization of their origin and expression pattern. Oncogene, 7,
1083-1091, 1992.
P.P. Pandolfi, M. Alcalay, M. Fagioli, D. Zangrilli, A. Mencarelli, D. Diverio, A. Biondi, F. Lo Coco, A. Rambaldi, F.
Grignani, C. Rochette-Egly, M.-P. Gaub, P. Chambon and P.G. Pelicci: Genomic variability and alternative splicings
generate multiple PML/RARα transcripts that encode aberrant PML proteins and PML/RARα isoforms in acute
promyelocytic leukaemias. EMBO J, 11, 1397-1408, 1992.
P.P. Pandolfi, Fr. Grignani, M. Alcalay, A. Mencarelli, A. Biondi, F. LoCoco, F. Grignani and P.G. Pelicci: Structure and
origin of the acute promyelocytic leukemia myl/RARα cDNA and characterization of its retinoid-binding and transactivation
properties. Oncogene, 6, 1285-1292, 1991.
F. Lo Coco, G. Avvisati, D. Diverio, A. Biondi, P.P. Pandolfi, M. Alcalay, G. de Rossi, M.C. Petti, A. Cant˘-Rajnoldi, D.
Pasqualetti, M. Nanni, S. Fenu, M. Frontani, and F. Mandelli: Rearrangements of the RARα gene in acute promyelocytic
leukaemia: correlations with morphology and immunophenotype. Brit J Haematol, 78, 494-499, 1991.
F. Lo Coco, G. Avvisati, D. Diverio, M.C. Petti, M. Alcalay, P.P. Pandolfi, A. Biondi, A. Rambaldi, M.L. Moleti, F. Mandelli,
P.G. Pelicci: Molecular evaluation of response to all-trans-retinoic acid therapy in patients with acute promyelocytic
leukaemia. Blood, 77, 1657-1659, 1991.
A. Biondi, A. Rambaldi, M. Alcalay, P.P. Pandolfi, F. Lo Coco, D. Diverio, V. Rossi, A. Mencarelli, L. Longo, D. Zangrilli,
G. Masera, T. Barbui, F. Mandelli, F. Grignani, P.G. Pelicci: RAR-α gene rearrangements as a genetic marker for
diagnosis and monitoring in acute promyelocytic leukaemia. Blood, 77, 1418-1422, 1991.
M. Alcalay, D. Zangrilli, P.P. Pandolfi, L.Longo, A. Mencarelli, A. Giacomucci, M. Rocchi, A. Biondi, A. Rambaldi, F. Lo
Coco, D. Diverio, E. Donti, F. Grignani, P.G. Pelicci: Translocation breakpoint of acute promyelocytic leukemia lies within
the retinoic acid receptor alpha locus. P Natl Acad Sci Usa, 88, 1977-1981, 1991.
L. Longo, P.P. Pandolfi, A. Biondi, A. Rambaldi, A. Mencarelli, F. Lo Coco, D. Diverio, L. Pegoraro, G. Avanzi, A. Tabilio,
D. Zangrilli, M. Alcalay, E. Donti, F. Grignani, P.G. Pelicci: Rearrangements and aberrant expression of the RARα gene
in acute promyelocytic leukemias. J Exp Med, 172, 1571-1575, 1990.
M. Alcalay, F. Antolini, W.J. Van de Ven, L. Lanfrancone, F. Grignani, P.G. Pelicci: Characterization of human and
mouse c-fes cDNA clones and identification of the 5' end of the gene. Oncogene, 5, 267-275, 1990
G. De Vita, M. Alcalay, M. Sampietro, M.D. Cappellini, G. Fiorelli, and D. Toniolo: Two point mutations are responsible
for G6PD polymorphism in Sardinia. Am J Hum Genet, 44, 233-240, 1989.
M. Alcalay and D. Toniolo: CpG islands of the X chromosome are gene associated. Nucleic Acid Res, 16 (20), 95279543, 1988.
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54. D. Toniolo, M. Persico, and M. Alcalay: A housekeeping gene on the X chromosome encodes a protein similar to
ubiquitin. P Natl Acad Sci Usa, 85, 851-855, 1988.
REVIEW ARTICLES
1. E. Colombo, M. Alcalay and P.G. Pelicci: Nucleophosmin and its complex network: a possible therapeutic target in
hematological diseases. Oncogene, 2011, 2011 Jun 9;30(23):2595-609.
2. N. Meani and M. Alcalay: Role of nucleophosmin in acute myeloid leukemia. Expert Reviews in anticancer Therapy, 2009,
Sep;9(9):1283-94
3. Guffanti, J.F. Reid, M. Alcalay and G. Simon: The meaning of it all:web-based resources for large-scale functional
annotation and visualization of DNA microarray data. Trends Genet 2002 Vol.18 (11): 589-592.
4. M. Alcalay, A. Orleth, C. Sebastiani, N. Meani, F. Chiaradonna, C. Casciari, M. T. Sciurpi, V. Gelmetti, D. Riganelli, S.
Minucci, M. Fagioli and P.G. Pelicci: Common themes in the pathogenesis of acute myeloid leukemia. Oncogene 2001
Sep 10;20(40):5680-94.
5. V. Brunel, M. Lafage-Pochitaloff, M. Alcalay, P.G. Pelicci, and F. Birg: Variant and masked translocations in acute
promyelocytic leukemia. Leukemia Lymphoma, 22 p.221-228, 1996.
6. M. Fagioli, Fr. Grignani, P.F. Ferrucci, M. Alcalay, A. Mencarelli, I. Nicoletti, F. Grignani, P.G. Pelicci: Effect of the acute
promyelocytic leukemia PML/RARα protein on differentiation and survival of myeloid precursors. Leukemia, 8 Supplement
1 S7-S11, 1994.
7. Fr. Grignani, M. Fagioli, P.F. Ferrucci, M. Alcalay, P.G. Pelicci: The molecular genetics of acute promyelocytic leukemia.
Blood Rev, 7, 87-93, 1993.
8. Fr. Grignani, M. Fagioli, M. Alcalay, L. Longo, P.P. Pandolfi, E. Donti, A. Biondi, F. Lo Coco, F. Grignani, P.G. Pelicci:
Acute Promyelocytic Leukemia: from genetics to treatment. Blood, 83(1), 10-25, 1993.
9. A. Biondi, A. Rambaldi, P.P. Pandolfi, M. Alcalay, L. Longo, V. Rossi, G. Giudici, F. Lo Coco and P.G. Pelicci: Molecular
genetics of the t(15;17) translocation in acute promyelocytic leukemia. Recent Results in Cancer Research, 131, 345-359,
1993.
10. P.P. Pandolfi, M. Alcalay, L. Longo, M. Fagioli, D. Zangrilli, Fr. Grignani, A. Mencarelli, A. Biondi, A. Rambaldi, F. Lo
Coco, F. Grignani and P.G. Pelicci: Molecular genetics of t(15;17) of acute promyelocytic leukemia (APL). Leukemia, 6
supplement 3, 120S-122S, 1992.
11. G.B. Bolis, R.M. Laurenzi, M. Alcalay: Le neoplasie maligne primitive multiple nell’uomo. Rivista di Anatomia Patologica e
di Oncologia, vol. 45 (3) 235-256, 1986.
BOOK CHAPTERS
1. S. Licciulli and M. Alcalay (2014). Nuclear Protein Pirin Negates the Cellular Senescence Barrier Against Cancer
Development. Tumor Dormancy, Quiescence, and Senescence, Volume 2: Aging, Cancer, and Noncancer Pathologies.
Edited by M.A. Hayat. Springer Science+Business Media Dordrech. DOI 10.1007/978-94-007-7726-2_14.
2. A. M. Gruszka and M. Alcalay (2013). PML/RARα Fusion Gene and Response to Retinoic Acid and Arsenic Trioxide
Treatment. Handbook of Therapeutic Biomarkers in Cancer. Edited by Sherry X. Yang and Janet Dancey. Pan Stanford
Publishing, ISBN: 9789814364652.
3. A. M. Gruszka and M. Alcalay (2012). Clinical and Biological Relevance of Gene Expression Profiling in Acute Myeloid
Leukemia, Myeloid Leukemia - Clinical Diagnosis and Treatment, Steffen Koschmieder and Utz Krug (Ed.), ISBN: 978953-307-886-1, InTech, Available from: http://www.intechopen.com/articles/show/title/clinical-and-biological-relevance-ofgene-expression-profiling-in-acute-myeloid-leukemia.
4. H. Muller and M. Alcalay: Near Genome Wide Expression Screening of Tumor Suppressor Pathways using Model Cell
Lines with Inducible Transcription Factors - Data Acquisition and Analysis. Artificial Intelligence and Heuristic Methods in
Bioinformatics. Edited by Paolo Frasconi and Ron Shamir. 16 NATO Science Series, IOS Press, pp 208-223, 2003.
5. H. Muller and M. Alcalay: Identification of Statistically Robust Gene Expression Patterns in Model Cell Lines and Their
Use in Functional Expression Data Mining. Microarrays and Cancer Research. Edited by Janet A. Warrington, Randy
Todd, and David Wong. Biotechniques Press, p45-60, 2002.
6. A. Biondi, A. Rambaldi, V. Rossi, A. Luciano, M. Cavana, F. Lo Coco, D. Diverio, P.P. Pandolfi, M. Alcalay, C.R. Bartram
and P.G. Pelicci: PCR approach for the evaluation of minimal residual disease in acute leukemia. Molecular Dagnosis and
Monitoring of Leukemia and Lymphoma, Borden, Goldman and Grignani eds., 211-219, 1994.
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7. M. Alcalay, Fr. Grignani, P.F. Ferrucci, M. Fagioli, A. Mencarelli, F. Grignani, P.G. Pelicci: The acute promyelocytic
leukemia PML/RARα protein affects differentiation and survival of myeloid precursor cells. Retinoids. From basic science
to clinical applications, Livrea/Vidali eds., Birkhauser Verlag (Basel), p. 357, 1994.
8. F. Grignani, M. Fagioli, M. Alcalay, L. Tomassoni, D. Rogaia, Fr. Grignani, P.G. Pelicci: Molecular Pathogenesis of Acute
Promyelocytic Leukemia. Hematopoietic Growth Factors, Oncogenes and Cytokines in Clinical Hematology, E. Cacciola,
A.B. Deisseroth and R. Giustolisi eds.Basel, Karger, vol 106, p. 148-159, 1994.
9. Fr. Grignani, M. Fagioli, M. Alcalay, P.F. Ferrucci, L. Tomassoni, D. Rogaia, C. Liberatore, M. Ruthardt, A. Mencarelli, F.
Grignani, P.G. Pelicci: Pathogenetic relevance of the acute promyelocytic leukemia specific PML/RARα fusion protein.
Molecular Diagnosis and Monitoring of Leukemia and Lymphoma, Aeres-Serono Symposia - Series Challanges of Modern
Medicine vol.2. Edited by E.C. Borden, J.M. Goldman and F. Grignani, 1993.
10. M. Alcalay, M. Fagioli, Fr. Grignani, P.F. Ferrucci, L. Tomassoni, D. Rogaia, C. Liberatore, M. Ruthardt, A. Mencarelli, F.
Grignani, P.G. Pelicci: Molecular genetics of the t(15;17) of acute promyelocytic leukamia. Molecular Diagnosis and
Monitoring of Leukemia and Lymphoma, Aeres-Serono Symposia - Series Challanges of Modern Medicine vol.2. Edited by
E.C. Borden, J.M. Goldman and F. Grignani, 1993.
11. Fr. Grignani, M. Fagioli, M. Alcalay, P.F. Ferrucci, L. Tomassoni, D. Rogaia, F. Grignani, P.G. Pelicci: The molecular
genetics of acute promyelocytic leukemia. Molecular Bases of Human Diseases, Elsevier Science Publishers B.V., 83-90,
1993.
12. A. Biondi, A. Rambaldi, P.P. Pandolfi, M. Alcalay, V. Rossi, G. Giudici, F. Lo Coco and P.G. Pelicci: Molecular genetics of
the t(15;17) translocation in acute promyelocytic leukemia. "Recent Results in Cancer Research", Springer Verlag Ed.,
Heidelberg, Germany, 1992.
13. M. Alcalay, L. Lanfrancone, M. Montanucci, G. Talamo, F. Grignani, P.G. Pelicci: Structure and function of the c-fes
oncogene. Oncogenes and Growth Factors in Oncohematology, Edited by M. Aglietta, G. Saglio, F.Gavosto. 97-100,
1988.
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