How much money we need for a
research project ?
• Financial support is a must
for undertaking any research
work.
• Unjustified request for fund
may lead to rejection of the
project
• Too less of finance may lead
to difficulty in completion of
a proposed project.
• Budget is a rational approach
towards requirement of fund
for research.
What is budget?
• A budget ensures that sufficient resources
(financial,
personnel,
and
infrastructure
/equipment) are available to complete the
research work that is being proposed.
• It should be based on a full analysis of research
project costs, and aim to get the sponsor to pay
for as many of those costs as possible, while
ensuring that both the sponsoring agency and
Institutional guidelines are followed.
Justification
• The budget justification narrative, required by
most agencies, is an important opportunity to
describe exactly how budget items will be used to
complete the study.
• A carefully constructed detailed budget increases
the likelihood of securing funding because it
documents the research team’s expertise to the
funding agency. An accurate budget is essential to
provide the necessary resources to conduct the
study.
At what stage it should be done?
• Preparing a research
proposal budget can seem
like an overwhelming
responsibility, especially
for new investigators.
• Preparing a detailed
budget during the
proposal stage can
minimize budget
management difficulties
once the project is funded.
Budget of a research project
Budget
Recurring
Expenditure
Non—
recurring
Expenditure
Nonrecurring Expenditure
Refer to one time expenditure
Infrastructure
Buliding
Animal
facility
Equipment
Equipment for Sample processing
Laboratory
(6-50K)
Refrigerated Micro, table
top (1-5L)
Centrifuge
Refrigerated heavy duty
(5-15L)
Ultracentrifuge
(> 50L)
Equipment for Storage
Common ( 30K)
Refrigerator
o
-20 C ( 1-1.5 L)
o
- 80 C
( 5-6 L)
Instruments for Quantification
Filter based
( 1-2 L)
ELISA reader
Filter based +
washer ( 3-4 L)
Wavelength
( 5-6 L)
Instruments for Quantification
Colorimeter (20-50K)
Photometry
Spectrocolorimeter
( 1-3 L)
Spectrophotometer
( 5-10 L)
Instruments for Quantification
• Spectro flurometer (8-10L): Measurement of
fluroescence.
• Nephlometer (2-3L) : Immuno-turbidimetry
• Chemiluminiscence (4-5L) :
• Multimode reader (12-15L)
Instruments for Quantification
Costly and big instruments
• Gas Chromatography (15-20L)
• HPLC (30-50L)
• Atomic absorption spectrophotometer (3040L)
• Flowcytometer (50-60L)
• Flowcytometer with cell sorter ( 75- 100L)
Equipments for Molecular Biology research
• Thermo cycler
•
•
•
•
personal (2-3L)
gradient ( 5-6L)
Gel Electrophoresis
(2-3 L)
Gel documentation System (5-6L)
Real time PCR (20-25 L)
Gene analysis system (40L)
Binocular +
camera ( 1-2L)
Microscopy
Fluorescent
microscopy
(>12L)
BUDGET FOR EQUIPMENT*
Generic name of equipment
S.No. with make and model
1.
2.
3.
4.
Real Time PCR (2 plex
with HRM)
Estimated cost
Imported /
Spare time for
(in foreign
Indigenous
other users (in %)
currency also)
Imported
Inverted microscope with
digital camera and
Imported
computer
Deep freezer (minus 20º C) Indigenous
Liquid nitrogen containers
Indigenous
(35 L x 2)
Rs 16.3 lakhs
(US$32,000)
50%
Rs 5 lakhs
(US$10,100)
50%
Rs 75,000
20%
Rs 80,000
20%
Justification for the proposed equipment
• The Department of Biochemistry, University College of Medical
Sciences, Delhi, where the proposed research project work will
be executed, has all the necessary equipments as mentioned in
“facilities/equipments being extended by parent institution”
except the above mentioned equipment.
• A Real time PCR machine (Q-PCR), critical for quantifying gene
expression patterns, is essential for achieving the objectives.
• The department is initiating cell / tissue culture facility and have
all the other equipments such as Laminer flow, CO2 incubator
except an inverted microscope with a digital camera attached to
a computer, for detailed image analysis.
• We will also require liquid nitrogen containers that will be used
for storing the cell lines.
• A dedicated deep freezer will be essential for storing tissue
culture material, temperature sensitive reagents and samples
for the project as the existing freezers are overloaded.
Recurring Expenditure
Repeating Expenditure
 Manpower
 Consumable
 Travel
 Contingency
 Overhead
• Expenditure to be mentioned Year wise separately for all the
above mentioned items depending on the experimental
protocol.
Manpower
Research Personnel
Help in planning, execution & data collection
• Research associate ( 23-24 K*)
• Medical officer ( 20K*)
• Senior research fellow ( 18-20 K* )
• Junior Research Fellow ( 16-18 K* )
• Research Assistant ( 21K *)
* + HRA @ as admissible
Technical Personnel
• Technical Officer/ Computer programmer
(29.5K*)
• Data entry operator/ Statistician (26.5 K*)
• Technician/ Data entry operator/ Nurse / Field
worker ( 17.5*)
• Lab. Assistant/ data entry operator Jr ( 15.5K*)
• Office assistant/ Driver (11.9 K*)
• Lab. Attendant/ Animal attendant ( 11K*)
* Consolidated salary
BUDGET FOR SALARIES / WAGES
BUDGET
Monthly
Designation
emolument
and no. of
s (in
persons
Rupees)
JRF (one)
15,600
Lab.
Attendant
(one)
6520
Total
–
(In rupees)
1st year
(m.m*)
2nd year
(m.m*)
3rd year
(m.m*)
Total
(m.m.)
–
–
–
–
1,87,200
(12)
1,87,200
(12)
1,87,200
(12)
5,61,600
(36)
78,240 (12) 78,240 (12) 78,240 (12)
2,65,440
2,65,440
2,65,440
2,34,720
(36)
7,96,320
Justification for the manpower requirement
(i) Junior Research Fellow (One) : The experiments and estimations
planned in the proposed research project will be carried out by the
investigators themselves / their post-graduate students. However,
to assist in the basic work like isolation of cells / DNA, preparation
of reagents, sample collection and processing and data
computation, a skilled JRF is required. Therefore, we propose to
recruit one JRF with M.Sc./M.Tech degree in Biochemistry/Life
Sciences/Medical Laboratory Technology/ Biotechnology (NET
qualified) with at least one year work experience in a
Biochemistry/Molecular biology laboratory and in-hand
experience in molecular biology techniques; pay as per DST rules.
(ii) Laboratory attendant : The proposed project involves collection of
large number of human samples. Transportation of samples from
O.P.D. to lab, processing, storage, analysis, and disposal of
samples, assisting tissue culture procedures like washing of
plasticware and glassware, autoclaving and cleaning of lab
accessories demands a full time helping hand. Therefore, we felt
the need of a laboratory attendant with some work experience in
laboratory work; consolidated pay as per DST rules.
Consumables
Consumables
To be calculated year wise as per sample size and other
variables.
•
•
•
•
Drugs /Medicines
Chemicals
Plastic wares/ glass wares
Disposables
Justification for consumables
The budget for biological reagents and chemicals constitute a major
expenditure for this project. Many of the reagents are not manufactured
in India and have to be imported either directly or through their
distributors. We will require reagents for
(i) Gas chromatography (GC) based analysis, that include HPLC grade
solvents, high quality nitrogen gas and standerds and GC vials. ECD
column used for GC can cater to approximately 300 – 400 samples. As
we will process more than seven hundred samples, we will require two
such ECD columns
(ii) Molecular biology work with special requirements for Real time PCR
(Taqman master mix and real time tubes), reagents / kits for DNA and
RNA isolation and PCR based reagents such as dNTPs, restriction
enzymes, Taq polymerase for gene polymorphism studies
(iii) Cell culture. For in vitro work, two cell lines will be commercially
procured.
(iv) As Real time PCR and cell culture are sensitive areas of research, we will
require at least two sets of dedicated autopipettes exclusively for each
area.
Plastic wares
• Autopipette : Routine use (3-5K)
For small volume (7-10K)
Multichannel (20K)
Electronic (50K)
• Other commonly required plastic wares
– ELISA plates, Microcentifuge tubes (2/1.5/0.5,0.2mL
@ 1K/1000) , Microtips (1000/100/10uL @ 1k/1000),
Tip boxes (1000/100/10uL @3K/12), centrifuge tubes
(15/50mL @ 1K/100 ), sample storage boxes
(2/1.5/0.5/0.2mL @ 3k/12), etc.
BUDGET FOR CONSUMABLE MATERIALS
S. No.
ITEM
1st year
BUDGET
2nd year
(In Rupees)
3rd year
Total
1.
Taqman master mix for
Real time PCR
40,000
40,000
30,000
1,10,000
2.
Taq polymerase, dNTPs,
Primers, etc for PCR
35,000
35,000
20,000
90,000
3.
Renal Proximal tubular
epithelial cell lines
60,000
40,000
–
1,00,000
30,000
30,000
20,000
80,000
30,000
30,000
20,000
80,000
4.
5.
Cell culture reagents like
FBS, RPMI, antibiotics
mixtures, etc
DNA / RNA isolation kits /
reagents
6.
Glass / Plastic ware
20,000
20,000
10,000
50,000
7.
Automatic pipette sets
(P1000, P200, P5-50, P220, P2.5)
40,000
40,000
–
80,000
8.
GC reagents (GC
standard, Hexane, Florisil)
70,000
70,000
40,000
1,80,000
9.
GC vials, Nitrogen gas
20,000
20,000
10,000
50,000
10.
ECD columns
50,000
90,000
–
1,40,000
3,95,000
(US$5000)
4,15,000
(US$5000)
1,50,000
(US$1500)
9,60,000
(US$11,500)
TOTAL B**
F***
Travel
• Expenditure for travel include :
i. Sample collection ( particularly for survey/
epidemiologic/ multi centric sampling).
ii. Travel expenditure for presenting research
work in scientific meeting ( inland only).
iii. Attending meeting for presenting annual
reports to funding agency.
BUDGET FOR TRAVEL
BUDGET
Travel (Only
inland travel)
(In rupees)
1st year
2nd year
3rd year
Total
10,000
20,000
20,000
50,000
Justification for travel
The budget for travel will be utilized for presentation of research findings in
different scientific meetings and DST grant monitoring meetings.
Contingency
• Expenditure under contingency include
i. Maintenance of equipment
ii. Stationary
iii. Printing material
iv. Petty expenditure
v. Postage
BUDGET FOR OTHER COSTS /
CONTINGENCIES
1st year
(In
rupees)
2nd year 3rd year Total
10,000
20,000
BUDGET
For
equipment
maintenance
and
contingency
20,000
50,000
Overhead
• A budgetary provision by the funding authority for
infrastructural expenses to the Investigator &
Project executing Institute.
• Electrical fixture, AC, refrigerator, furniture,
telephone/ internet bill, common minor equipment,
contingent salary for manpower assistance, gas etc.
• Calculated in terms of percentage of the total
budget excluding equipments.
BUDGET ESTIMATES: SUMMARY
Item
1st year
A
BUDGET
2nd year
3rd year
(In Rupees)
Total
2,65,440
2,65,440
7,96,320
Recurring
1.
Salaries/Wages
2. Consumables
2,65,440
3,95,000
(US$5000)
4,15,000
(US$5000)
1,50,000
(US$1500)
9,60,000
(US$11,500)
3. Travel
4. Other costs
(Maintenance of
equipment and
contingency)
10,000
10,000
20,000
20,000
20,000
20,000
50,000
50,000
22,85,000
(US$42,100)
–
–
22,85,000
(US$42,100)
Total (A+B)
FEC*
29,65,440
(US$47,100)
7,20,440
(US$5000)
4,55,440
(US$1500)
41,41,320
(US$53,600)
C. Overhead (20%)
Grand total
5,93,088
35,58,528
1,44,088
8,64,528
91,088
5,46,528
8,28,264
49,69,584
B. Equipment
Some funded projects by various funding agency
• The effect of low glycemic composite flour on
diabetic and normal subjects
• Pharmacokinetic Profile of Nasal
Corticosteroid and Oral antiHistaminic used in
combination for treatment of Allergic Rhinitis
in rural India.
• Genotype phenotype correlation in Wilson's
Disease within families
• Study of New Gemostatic Gene Polymorphisms in
Young Patients from North India with acute
Myocardial Infraction
• Genetic, Metabolic and Hormonal Profile in
Obstructive Sleep Apnea Syndrome
• Invitro inhibition of T cell Cytokines production by
methotrexate: Can it predict response to
methotrexate in patients with rheumatoid
arthritis
• Gene-Environment interactions and susceptibility
to breast cancer.
Some ICMR funded Project
• MOLECULAR BASIS OF AGE-RELATED DECLINE IN LEARNING AND
MEMORY ;M.S.KANUNGO
BANARAS HINDU UNIVERSITY, VARANASI
• SELENOPROTEINS AND ANTIOXIDANTS IN PATHOGENESIS OF
ALZHEIMER'S DISEASE : P.RANI, P.S.G.COLLEGE OF TECHNOLOGY,
COIMBATORE
• PATTERNS OF FOOD ALLERGEN- SPECIFIC CYTOKINE PRODUCTION BY T
LYMPHOCYTE SUBSETS OF ATOPIC CHILDREN : B.P.CHATTERJEE
INDIAN ASSOCIATION FOR CULTIVATION OF SCIENCE ,KOLKATA
• A STUDY ON THE NOVEL ANALGESIC EFFECT OF INTRASPINAL LOPERAMIDE
IN A MURINE MODEL OF POSTSURGICAL PAIN : S.K.B.RAY, ALL INDIA
INSTITUTE OF MEDICAL SCIENCES ,NEW DELHI
• IS OXIDATIVE STRESS AND LIPID PEROXIDATION AN VIABLE MECHANISM
OF PERPETUATION OF VASCULAR PATHOLOGY DUE TO
HYPERHOMOCYSTEINEMIA? SEEMA BHARGAVA, SIR GANGARAM
HOSPITAL , NEW DELHI
ICMR funded project
• EVALUATION OF PLATELET RECEPTOR POLYMORPHISM IN PATIENTS WITH
CORONARY ARTERY DISEASE, ENU SAXENA, ALL INDIA INSTITUTE OF
MEDICAL SCIENCES , NEW DELHI
• ESTABLISHMENT OF MOLECULAR CHARACTERIZATION OF HEMOLOGLOBINOPATHIES AND PRENATAL DIAGNOSIS OF THALASSEMIA AND SICKLE
CELL DISEASE AT REGIONAL CENTRES ,M.K.GHOSH,N.R.S.MEDICAL
COLLEGE AND HOSPITAL,KOLKATA
• EVALUATION OF THE BENEFICIAL EFFECTS OF TAURINE SUPPLEMENTATION
IN PATIENTS WITH DIABETES MELLITUS ,S.SETHUPATHY,RAJAH MUTHIAH
MEDICAL COLLEGE AND HOSPITAL, ANNAMALAI NAGAR
•
Title of the Project :
Association of Common Variants of Inflammatory
Cytokine Genes with Risk of Nephropathy in Type 2
Diabetes among Asian Indians
Objective :
Eight single nucleotide polymorphisms (SNPs) of
pro-inflammatory cytokine genes (CCL2, TGFB1,
IL8, CCR5, and MMP9) were to be genotyped.
Sample :
Two independently ascertained type 2 diabetic
cohorts with nephropathy (n=200), without
nephropathy (n=200) and control ( n=200).
Methodology:
• DNA will be isolated by DNA isolation kit.
• Genotyping will be carried out by PCR- RFLP
using a thermocycler.
• hsCRP will be estimated by ELISA using
commercially available kit.
( Note: Collaborative dept. have ELISA reader &
do not have a thermocycler. 50 sample DNA
isolation kit costs = Rs 5 K, 96 well hsCRP kit
costs Rs 12K)