SureSelect Decission Tree
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XT SureSelect™ Decision Tree Focus your next-gen sequencing on DNA that matters All Exon v1-38Mb Kinome X All Exon v2-44Mb Whole Exome Human SureSelectTM Decision Tree All Exon 50Mb Custom Capture Custom Capture Click Here Human Kinome Chromosome DNA Custom Capture Non Human What do you want to capture? Mouse Exome RNA Non Human Custom Capture SureSelect All Exon Kits Whole Exome in one capture! Crack the Codes of Mendelian and other Diseases Less sequencing to results Up to 20x more samples per run – compared to Whole Genome Sequencing All Exon 50Mb Performance 100% 90% 80% 70% 60% 50% 40% 30% 20% 10% 0% 76.3% 85.1% 96.7% 87.9% 77.5% Most comprehensive coverage in the market Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect Human All Exon Kits Comprehensive coverage Target Size CCDS (Nov. 2010) RefSeq Genes (Nov.2010) miRNA (miRBase v14) Ensembl (Aug.2010) GENCODE v4 Addition of Custom Exome Developed with All Exon v1 All Exon v2 All Exon 50Mb CCDS Sept. 2008 CCDS Sept. 2008 + additional RefSeq content including CCDS Sept. 2009 exons GENCODE and Sanger (includes CCDS and Broad defined v2 content as well) 38Mb 89.6% 85.9% 90.0% 79.9% 80.9% 44Mb 98.2% 96.9% 90.0% 90.9% 92.9% 50Mb 99.5% 99.0% 92. 8% 96.2% 97.0 Yes Yes Yes Broad Broad Sanger Human All Exon kits can be customized (PLUS) with up to 6.8Mb additional custom content Human All Exon kits can be multiplexed on SOLiD4 and HiSeq2000 Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect All Exon 50Mb Reactions SureSelect Human SureSelect XT Human All Exon Kit 50Mb All Exon 50Mb SureSelect Human All Exon Kit 50Mb PLUS 5 rxns G3370A G7544A x 10 rxns G3370B G7544B x 25 rxns G3370C G7544C x 50 rxns G3370D G7544D x 100 rxns G3370E G7544E x 250 rxns G3370F G7544F x 500 rxns G3370G G7544G x 1000 rxns G3370H G7544H x 2000 rxns G3370J G7544J x 5000 rxns G3370K G7544K x 10000 rxns G3370L G7544L x •The PLUS option allows addition of up to 6.8Mb of custom content using the eArray tool •The XT option includes also kits for gDNA prep and library prep Add the following Sequencing Platform extension to the Part Number Illumina GA/HiSeq 001 SOLiD 002 454 003 Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect All Exon v1 Kits – 38Mb Reactions SureSelect Human SureSelect XT Human SureSelect Human All Exon Kit All Exon All Exon Plus Kit SureSelect XT Human All Exon Plus Kit2 5 rxns G3362A G7540A G3363A G7541A 10 rxns G3362B G7540B G3363B G7541B 25 rxns G3362C G7540C G3363C G7541C 50 rxns G3362D G7540D G3363D G7541D 100 rxns G3362E G7540E G3363E G7541E 250 rxns G3362F G7540F G3362F G7541F 500 rxns G3362G G7540G G3363G G7541G 1000 rxns G3362H G7540H G3363H G7541H 2000 rxns G3362J G7540J G3363J G7541J 5000 rxns G3362K G7540K G3363K G7541K 10000 rxns G3362L G7540L G3363L G7541L •The PLUS option allows addition of up to 6.8Mb of custom content using the eArray tool •The XT option includes also kits for gDNA prep and library prep Add the following Sequencing Platform extension to the Part Number Illumina GA/HiSeq 001 SOLiD 002 454 003 Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect All Exon v2 Kits – 44Mb Reactions 5 rxns 10 rxns 25 rxns 50 rxns 100 rxns 250 rxns 500 rxns 1000 rxns 2000 rxns 5000 rxns 10000 rxns SureSelect Human SureSelect XT SureSelect Human All Exon v2 Kit Human All Exon v2 All Exon v2 Plus Kit G3353A G3353B G3353C G3353D G3353E G3362F G3353G G3353H G3353J G3353K G3353L G7542A G7542B G7542C G7542D G7542E G7542F G7542G G7542H G7542J G7542K G7542L x x x x x x x x x x x •The PLUS option allows addition of up to 6.8Mb of custom content using the eArray tool •The XT option includes also kits for gDNA prep and library prep Add the following Sequencing Platform extension to the Part Number Illumina GA/HiSeq 001 SOLiD 002 454 003 Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect X-Chromosome Reactions 5 rxns SureSelect Target Enrichment System, Demo Kit (XChromosome) SureSelect XT Human X-Chr G4459A G7560A Add the following Sequencing Platform extension to the Part Number Illumina GA/HiSeq 001 SOLiD 002 454 003 •The XT option includes also kits for gDNA prep and library prep Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect Kinome Discovery and Profiling of Biomarkers for Disease and Drug Response Definition of SureSelect Human Kinome Kit: 3.2Mb (incl. UTRs): 518 putative kinases 12 PI3K domain-containing genes 13 diglyceride kinases 6 PI3K regulatory components 9 inositol polyphosphate Kinases 9 PIP4/PIP5 Kinases 28 genes frequently mutated in human cancer 19 genes specifically known to be mutated in breast cancer 612 genes total G. Manning et al Science (2002) Original content defined by Prof. René Bernards – NKI Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect Human Kinome Kit – DNA Reactions SureSelect Human All Kinome Kit SureSelect XT Human Kinome 5 rxns 10 rxns 25 rxns 50 rxns 100 rxns 250 rxns 500 rxns 1000 rxns 2000 rxns 5000 rxns G3365A G3365B G3365C G3365D G3365E G3365F G3365G G3365H G3365J G3365K G7562A G7562B G7562C G7562D G7562E G7562F G7562G G7562H G7562J G7562K 10000 rxns G3365L G7562L •The XT option includes also kits for gDNA prep and library prep Add the following Sequencing Platform extension to the Part Number Illumina GA/HiSeq 001 SOLiD 002 454 003 Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect Custom Kits Capture any custom genomic regions (introns, exons, UTRs, regulatory, etc.) Ideal for biomarkers discovery and profiling (e.g. cancer) Ideal for custom SNP follow-up Ideal for characterization of large sample cohorts Only pay what you capture, scalable from 0.2 to 6.8Mb (sweet spot for 3rd Gen Seq) o <0.2 Mb o 0.2 – 0.5Mb o 0.5 – 1.5Mb o 1.5 – 3Mb o 3 – 6.8Mb COMING SOON! – Custom Kits up to 34Mb IMAGE: Nature Immunology Volume: 11, Pages: 197–206 (2010) Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect Custom Kits – DNA Add the following Sequencing Platform extension to the Part Number Illumina GA/HiSeq 001 SOLiD 002 454 003 Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow Expanding Exome Design to Animal Models Mouse All Exon 100% 90% 80% 70% 60% 50% 40% 30% 20% 10% 0% Exon definition derived from Ensembl + RefSeq Designed against mm9 reference from UCSC Number of genes covered: 24,306 Number of exons: 221,784 Total size of design: 49.6 MB Performance Mouse All Exon Design with 5Gb sequencing % Reads OnUniformity (3/4 Target +/- 200bp mean with upper tail) Promega C3H DBA % Bases 1X Coverage PWK % Bases 10X Coverage 15NIH36a % Bases 20X Coverage 15NIH49a Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect Mouse All Exon Kits Reactions SureSelect XT SureSelect Mouse Mouse All Exon All Exon Kit Kit 5 rxns G7500A G7550A 10 rxns G7500B G7550B 25 rxns G7500C G7550C 50 rxns G7500D G7550D 100 rxns G7500E G7550E 250 rxns G7500F G7550F 500 rxns G7500G G7550G 1000 rxns G7500H G7550H 2000 rxns G7500J G7550J 5000 rxns G7500K G7550K 10000 rxns G7500L G7550L •The XT option includes also kits for gDNA prep and library prep Add the following Sequencing Platform extension to the Part Number Illumina GA/HiSeq 001 SOLiD 002 454 003 Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow Performance of SureSelect RNA Target Enrichment Enriched library Gene Expression Levels R2=0.885 Unenriched library High correlation to unenriched RNA library = enrichment process doesn’t create any bias – reliable gene expression results! Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow Performance of SureSelect RNA Target Enrichment Enriched Library 1 Gene Expression Levels R2=0.998 Enriched library 2 High Reproducible process = reliable gene expression results Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect Custom Kits - RNA SureSelect Reactions <200kb (MP1) 10 rxns 25 rxns 50 rxns 100 rxns 250 rxns 500 rxns 1000 rxns 2000 rxns 5000 rxns 10000 rxns G7581B G7581C G7581D G7581E G7581F G7581G G7581H G7581J G7581K G7581L Add the following Sequencing Platform extension to the Part Number 200kb - 499kb 500kb-1.49Mb 1.5Mb-2.99Mb 3.0Mb-6.8Mb (MP2) (MP3) (MP4) (MP0) G7582B G7582C G7582D G7582E G7582F G7582G G7582H G7582J G7582K G7582L G7583B G7583C G7583D G7583E G7583F G7583G G7583H G7583J G7583K G7583L G7584B G7584C G7584D G7584E G7584F G7584G G7584H G7584J G7584K G7584L G7585B G7585C G7585D G7585E G7585F G7585G G7585H G7585J G7585K G7585L Illumina GA/HiSeq 001 SOLiD 002 Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow SureSelect RNA Capture Human Kinome Kit Reactions SureSelect RNA Capture Human Kinome Kit 5 rxns 10 rxns 25 rxns 50 rxns 100 rxns 250 rxns 500 rxns 1000 rxns 2000 rxns 5000 rxns G7580A G7580B G7580C G7580D G7580E G7580F G7580G G7580H G7580J G7580K 10000 rxns G7580L Add the following Sequencing Platform extension to the Part Number Illumina GA/HiSeq 001 SOLiD 002 Click to go back to the decision tree Agilent SureSelect™ Platform Enabling Products for the Next-Generation Sequencing Workflow All Exon v1 (38Mb) Kinome X All Exon v2 (44Mb) Whole Exome All Exon 50Mb Custom Capture Human SureSelectTM Decision Tree Custom Capture Human Kinome Chromosome DNA Custom Capture Non Human What do you want to capture? Mouse Exome RNA Non Human Custom Capture
