Suppl. Fig. 1 Cellular-growth in Raji cells treated by OLL and SAL
Growth curves of Raji cells treated with OLL or SAL. Raji cells were treated with
OLL 10 µg/mL ( ■ ), 50 µg/mL ( ▲ ), 100 µg/mL ( ♦ ), 200 µg/mL ( ● ), and
SAL 10 µg/mL (×), 100 µg/mL ( + ), respectively. After 6h, 12h, 24h, 48h incubation,
the cells were counted. ◊ , control.
Cytokine gene / GAPDH
2.5
SAL-
2
SAL+
1.5
1
low
low
0.5
N.D.
0
INF-γ
TNF-α
IL-1β
IL-6
N.D.
IL-10
IL-12B
Suppl. Fig. 2 Expression of cytokine genes in Raji cells treated by SAL
Total RNAs were extracted from SAL-treated and –untreated Raji cells, and then subjected to
quantitative RT-PCR performed with primers specific for IFN-γ, TNF-α, IL-1β, IL-6, IL-10,
IL-12B and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (control). The copy numbers
of candidate gene mRNA were standardized against that of GAPDH in each sample. Data, expressed
as relative amounts, represents the mean value ±S.E. for two independent experiment.
Suppl. Table 1
Genes
Crossing Point Values of Detection of TNFR1 / 2 genes in Raji cells
Average of crossing point valuea)
TNFR1
26.84 ± 0.76
TNFR2
31.18 ± 0.17
GAPDHb)
20.01 ± 0.61
a) Crossing point values are obtained from the Light cycler 480 software Ver. 1.2, representing
the PCR cycle at which an increase in fluorescence intensity above a base line signal is first
detected. Each value represents the mean value ± S.E. for three different experiments performed
in duplicate. b) Control gene: glyceraldehyde 3-phosphate dehydrogenase (GAPDH).