Eka Susilawati Sukmawati Ayu Martalia Wahyu Hasan Ramdan Tiara Pelangi A Sri Aryani (10506106) (10507032) (10507034) (10507056) (10507066) (10507076) Three Glycolysis reactions have such a large negative DG that they are essentially irreversible. Hexokinase (or Glucokinase) Phosphofructokinase Pyruvate Kinase. Pyruvate Kinase (last step of Glycolysis) catalyzes: phosphoenolpyruvate + ADP pyruvate + ATP Piruvat Kinase adalah enzim yang mengkatalisis langkah irreversibel ketiga pada glikolisis Piruvat Kinase menghasilkan ATP dan Piruvat Bila muatan energi tinggi , ATP secara allosterik menginhibisi Piruvat Kinase untuk memperlambat glikolisis Piruvat Kinase terfosforilasi (kurang aktif) H2O Kadar glukosa darah merah - PI + ADP ATP Piruvat Kinase terdesfosforilasi (lebih aktif) Fosfoenolpiruvat + ADP +H+ + Fruktosa 1,6bisfosfat ATP Alanin Piruvat + ATP Alanin secara allosterik menghambat piruvat kinase Bila kadar glukosa darah menurun, glukagon memicu kaskade AMP siklik yang menyebabkan fosforilasi Piruvat Kinase, yang mengurangi aktivitasnya Fosforilasi ini seperti enzim bifungsi yang mengontrol kadar fruktosa 2,6-bisfosfat Fruktosa 2,6-bisfosfat dibentuk melalui fosforilasi senyawa fruktosa 6-fosfat oleh enzim fosfofruktokinase-2 dalam keadaan glukosa yang berlimpah, konsentrasi senyawa fruktosa 2,6-bisfosfat akan meningkat sehingga merangsang glikolisis dengan mengaktifkan fosfofruktokinase-1 dan menghambat fruktosa-1,6bisfosfatase. Dalam keadaan kekurangan glukosa, glukoneogenesis dirangsang oleh penurunan konsentrasi fruktosa 2,6-bisfosfat yang kemudian menghilangkan aktivitas fosfofruktokinase-1 dan meniadakan penghambatan kerja fruktosa-1, 6bisfosfatase. Pyruvate Carboxylase (pyruvate oxaloactate) is allosterically activated by acetyl CoA. [Oxaloacetate] tends to be limiting for Krebs cycle. G lucose-6-phosphatase glucose-6-P glucose G luconeogenesis G lycolysis pyruvate fatty acids acetyl C oA oxaloacetate ketone bodies citrate K rebs C ycle Enzymes relevant to these pathways that are phosphorylated by Protein Kinase A include: Pyruvate Kinase, a glycolysis enzyme that is inhibited when phosphorylated. CREB (cAMP response element binding protein) which activates, through other factors, transcription of the gene for PEP Carboxykinase, leading to increased gluconeogenesis. A bi-functional enzyme that makes and degrades an allosteric regulator, fructose-2,6-bisphosphate. PFK activity in the presence of the globally controlled allosteric regulator fructose-2,6-bisphosphate is similar to that at low ATP. Fructose-2,6-bisphosphate promotes the relaxed state, activating Phosphofructokinase even at high [ATP]. Thus activation by fructose-2,6-bisphosphate, whose concentration fluctuates in response to external hormonal signals, supersedes local control by [ATP].