Microbial Nutrition and Growth Nutrition = Obtaining Required Substances from the Environment Essential Nutrients Must be Provided for an Organism to Survive and Reproduce Nutrients • Inorganic nutrients– atoms, ions or molecules that contains a combination of atoms other than carbon and hydrogen – metals and their salts (magnesium sulfate, ferric nitrate, sodium phosphate), gases (oxygen, carbon dioxide) and water • Organic nutrients- contain carbon bonded to hydrogen and are usually the products of living things – methane (CH4), carbohydrates, lipids, proteins, and nucleic acids Macronutrients • Required in Large Quantities • Play principle roles in cell structure and metabolism • Proteins (source of amino acids) • Carbohydrates Micronutrients Needed in Small Amounts – like Minerals Points about Bacterial Cytoplasm • • • • Mostly water Large proportion of protein 97% of dry weight is organic matter 96% of bacterial cell is composed of C, H, N, O, P and S Challenge for Bacteria How to get enough nutrients in forms that they can use to make cell components Bacteria Must Make • • • • Proteins Carbohydrates Lipids Nucleic Acids Sources of Essential Nutrients • Carbon – obtain in organic form, or reduce CO2 • Nitrogen – Fix N2 or obtain as NO3-NO2-, or NH3 • Oxygen – Atmospheric or dissolved in water • Hydrogen – Minerals, water, organic compounds Nutrient Sources - Continued • Phosphorous – Mineral deposits • Sulfur – Minerals, H2S • Metal Ions - Minerals Mineral Nutrients Important in Microbial Metabolism • Potassium – essential to protein synthesis and membrane function • Sodium – used in some types of cell transport • Calcium – cell wall and endospore stabilizer • Magnesium – component of chlorophyll; membrane and ribosome stabilizer • Iron – component of proteins of cellular respiration • Zinc, copper, nickel, manganese, etc. Growth Factors • Organic compounds that cannot be synthesized by an organism & must be provided as a nutrient – essential amino acids, vitamins Nutritional Types • Autotrophs - use CO2, an inorganic gas as carbon source • Heterotrophs - obtain carbon in an organic form made by other living organisms Autotrophs – “Self-Feeding” • Phototrophs use light energy to reduce carbon or make ATP • Chemotrophs use energy stored in inorganic chemical bonds to reduce carbon or make ATP Heterotrophs • Obtain reduced carbon compounds made by another organism • Chemoheterotrophs – oxidize reduced carbon to make ATP Two Kinds of Bacterial Heterotrophs • Saprobes – Obtain nutrients from dead, decaying matter • Parasites – Feed off a host organism Environmental Influences on Microbial Growth • • • • Temperature Oxygen requirements pH Barometric pressure 3 Cardinal Temperatures • Minimum temperature • Maximum temperature • Optimum temperature 3 Temperature Adaptation Groups 1. Psychrophiles – optimum temperature below 15oC, capable of growth at 0oC 2. Mesophiles – optimum temperature 20o-40oC, most human pathogens 3. Thermophiles – optimum temperature greater than 45oC Ecological Groups by Temperature of Adaptation Oxygen in the Microbial Environment • Oxygen required by aerobic species (Bacillus, Pseudomonas) but produces toxic by-products; these species have efficient de-tox enzymes • Facultative anaerobes can exist in presence of oxygen but have no requirement for it (E. coli, Staphylococcus, etc.) Anaerobes – no Need for Oxygen • Strict anaerobes cannot tolerate oxygen (Clostridium sp.) • Aerotolerant anaerobes have atypical oxygen detox systems (Lactobacillus sp.) • Capnophiles require higher CO2 pressures (Neisseria, Brucella, S. pneumoniae) pH Effects on Growth • Acidophiles require low pH (Thermoplasma) • Alkalinophiles require high pH (Proteus) Osmotic Effects • Most microbes exist under hypotonic or isotonic conditions • Halophiles – require a high concentration of salt • Osmotolerant – do not require high concentration of solute but can tolerate it when it occurs Miscellaneous Environmental Factors • Barophiles require high environmental pressure (like deep sea Archae) • Dehydrated Cell Stages – Spores • Extreme radiation conditions Dinococcus radiodurans Ecological Relationships • Symbiosis – existing together • Mutualism – both parties benefit • Commensalism – one party benefits without impacting the other • Parasitism – one party benefits at expense of the other • Synergism – multiple organisms working together Microbial Biofilms • Biofilms result when organisms attach to a substrate by some form of extracellular matrix that binds them together in complex organized layers • Dominate the structure of most natural environments on earth • Microorganisms communicate and cooperate in the formation and function of biofilms – quorum sensing Ecological Relationships Continued • Antagonism – one party acts to inhibit or eliminate the other • Example of antagonism: Antibiosis – producing substances toxic to other organisms Microbial Growth Growth of a population at an exponential rate under optimal conditions Stages in Normal Growth • • • • Lag phase Exponential/log phase Stationary phase Death or decline Mathematics of Population Growth Growth Curve Methods for Monitoring Growth • Dilution Plating • Turbidity analysis using spectrophotometer • Direct count with hemacytometer • Optical detection – Coulter Counter Turbidity Direct Microscopic Count Electronic Counting It is Ideal to Perform Most Manipulations of a Culture When it is Growing in Log Phase