Foreign Body Reaction The presence of the implant changes the healing response, and this is called the Foreign Body Reaction. FBR consists of: protein adsorption multinucleated foreign body giant cells fibrous encapsulation B. Amsden CHEE 340 Components of Plasma Component Typical Concentration (mg/100 ml) protein 7250 salts 325 lipids 828 glucose 90 urea 25 carbohydrates 85 B. Amsden CHEE 340 Proteins are polyamides, composed of amino acids B. Amsden CHEE 340 Soluble Proteins (A) A space-filling model of myoglobin with hydrophobic amino acids shown in yellow, charged amino acids shown in blue, and others shown in white. The surface of the molecule has many charged amino acids, as well as some hydrophobic amino acids. (B) A cross-sectional view shows that mostly hydrophobic amino acids are found on the inside of the structure, whereas the charged amino acids are found on the protein surface. B. Amsden CHEE 340 albumin Protein Adsorption CHEE 872 B. Amsden Protein Adsorption and Cell Response Vroman Effect From: An Introduction to Tissue-Biomaterial Interactions, Wiley, 2002. B. Amsden CHEE 340 Wound Healing in Presence of Biomaterial protein adsorption mediates neutrophil and macrophage response Den Dunnen et al. J Biomed Mater Res 36 (1997) 337 B. Amsden CHEE 340 Frustrated Phagocytosis B. Amsden CHEE 340 Foreign Body Response continuing presence of an implant may result in the attainment of a final steady-state condition called resolution there are 3 possible outcomes for the implant : » resorption » integration » encapsulation (fibrosis) B. Amsden CHEE 340 Example in vivo response to implanted biodegradable elastomer B. Amsden CHEE 872 Experimental Results poly(trimethylene carbonate) elastomer implanted s.c. in rats B. Amsden CHEE 872