Genetics The study of potentials of passing information from one generation to the next Gregor Mendel • • • • • • “Father” of genetics Studied pea plants Used contrasting features Parents “bred true” Kept good records Studied one trait at a time Mendel’s pea experiment • • • • True breeding purple flower plants True breeding white flower plants F1 generation F2 generation Terminology • Gene – unit of inheritance made of DNA • Genes occur in pairs except in the egg and sperm • An allele is a different form of the same gene • Black hair and brown hair are alleles of the hair color gene Genes • Dominant gene is one that can overshadow another gene. Signified with capital letter, e.g. “A” • Recessive gene is the subordinate gene that can be suppressed by the dominant gene. Signified with a lower-case letter, e.g. “a” Genes • Homozygous – since genes occur in pairs, this is when both genes for the trait are the same • AA is homozygous dominant • aa is homozygous recessive Genes • Heterozygous – genes are alleles or different for a given trait • Aa is heterozygous • Carrier is sometimes used to indicate that someone is heterozygous Genes • Genotype is your genetic makeup • For example, you might be heterozygous for hair color, Bb, one gene for black hair, and one for blonde hair Genes • Phenotype is your physical appearance • Your phenotype for hair color may be brown One gene problem • Let P = Purple flower plants, or • Let p = white flower plants, or • The parent generation included true breeding purple flower plants that must have been PP (homozygous dominant) in genotype • The parent generation included true breeding white flower plants that must have been pp (homozygous recessive) in genotype Parents • PP plant can only make a gamete with the first “P” gene, or the second “P” gene • (See example using E instead of P) • pp plant can only make a gamete with its first “p” gene, or the second “p” gene • (See example using e instead of P) F1 generation • If these two parents fertilize to create offspring, F1, or first generation, what genotypes and phenotypes would be possible in the F1 generation? • Use a mathematical Punnet square to find the probabilities F1 generation Gamete with “P” gene – parent 1 Gamete with “P” gene – parent 1 Gamete with “p” Offspring is Offspring is gene – parent 2 heterozygous – heterozygous – “Pp” “Pp” Gamete with “p” Offspring is Offspring is gene – parent 2 heterozygous – heterozygous – “Pp” “Pp” F1 generation • 100% of the offspring would have a genotype of Pp, or be heterozygous • 100% of the offspring would have a phenotype of purple flowers, since purple is dominant over white F2 generation • If you take two plants from the F1 generation (Pp) and cross them, what would be the genotypic and phenotypic ratios of the next, or F2 generation? F2 generation Gamete with “P” gene – parent 1 Gamete with “P” gene – parent 2 Offspring is homozygous dominant – “PP” Gamete with “p” Offspring is gene – parent 2 heterozygous – “Pp” Gamete with “p” gene – parent 1 Offspring is heterozygous – “Pp” Offspring is homozygous recessive – “pp” F2 generation • Note that the four squares in the Punnet square do not represent four different offspring, but rather the statistical chances that any ONE offspring has a given trait • Genotypic ratios are 1 homozygous dominant : 2 heterozygous : 1 homozygous recessive • Phenotypic ratios are 3 purple : 1 white Try this problem • E = unattached earlobes • e = attached earlobes • Problem one: Both parents have unattached earlobes. However, they had a son with attached earlobes. What are the chances that their next child will have unattached earlobes? Try this problem • E = unattached earlobes • e = attached earlobes • Problem two: One parent has unattached earlobes and the other has attached earlobes. They had a daughter with attached earlobes. What are the genotypes of the parents? What are the chances that their next child will have unattached earlobes? Autosomal Dominant • The trait or disease is the result of a dominant gene Autosomal Recessive • The trait or disease is the result of a recessive gene (e.g. cystic fibrosis) Incomplete dominance • The dominant gene is not strong enough to completely overshadow the recessive gene • Example: If a red flower plant (dominant) crosses with a white flower plant (recessive) and the offspring have pink flowers (heterozygous). Codominance • No clear dominant or recessive gene • Genes are equal, or codominant • If genes for blood type A and blood type B are codominant and you have one gene of each, your blood type is AB Blood typing practice • Genes for blood types A and B are dominant over type O • Genes for blood types A and B are codominant with each other • If mom has blood type A and dad has blood type B, and they have a son with blood type O, what are the genotypes of these 3 people? What are the statistical chances that their next child has blood type AB? Sex-Linked traits • The sex of the person has not mattered up until this point working on the genetics problems. Now it does. • These sex-linked problems are such that the gene for the particular trait is located on the X chromosome only. • Recall that males are XY and females are XX. Colorblindness is sex linked • • • • Colorblind is a recessive trait Carried on the X chromosome only Let B = normal vision and b = color blind Mom and dad both have normal vision. However, their second child was a colorblind son. What are the genotypes of the parents and what are the chances that their next son/daughter is colorblind?