Maria Chatzinikolaidou
Biomaterials
Dept. Materials Science and Technology
University of Crete, P.O. Box 2208 GR-
71303 Heraklio, Crete, Greece mchatzin@materials.uoc.gr

 In vitro biocompatibility study of biomaterials, including cell adhesion, viability, proliferation and morphology of mammalian cell lines and stem cells
 Investigation of the osteoinductive potential of implantable biomaterials  bone tissue repair
 Development of immobilization techniques for proteins on biomaterials surfaces
 Adsorption and desorption studies of proteins on biomaterials surfaces

Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest
Biomaterial: organic-inorganic composite material, structured by twophoton polymerization
After 4 hours After 3 days
In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki

Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest
Biomaterial: organic-inorganic composite material, structured by twophoton polymerization
After 6 days
In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki

Cell source: Bone marrow mesenchymal stem cells from donors’ iliac crest
Biomaterial: organic-inorganic composite material – meshes structured by two-photon polymerization
After 4 hours
In collaboration with Prof. Maria Vamvakaki, Dr. Maria Farsari and Prof. Eleni Papadaki

Early event Late event
Extracellular factors and integrin-derived signaling trigger activate actin polymerization at the leading-edge membrane (as an early event ) and the formation of focal adhesions (as a later event )
Biomaterials: The Intersection of Biology and Materials ScienceJohnna S. Temenoff, Antonios G. Mikos

Cell morphology of MC3T3-E1
Visualization of vinculin and actin cytoskeleton by means of confocal microscopy
Cell source: mouse pre-osteoblasts MC3T3-E1
Biomaterial: organic-inorganic composite material
Overlay of double staining with ΤRΙTC-conjugated falloifin and
FITC-conjugated anti-vinculin magnification 20x

Cell morphology
Visualization of vinculin and actin cytoskeleton by means of confocal microscopy
Cell source: mouse pre-osteoblasts MC3T3-E1
Biomaterial: organic-inorganic composite material
Overlay of double staining with ΤRΙTC-conjugated falloifin and
FITC-conjugated anti-vinculin magnification 40x

Collaborations
Prof. Maria Vamvakaki, UoC
Dr. Maria Farsari, Prof. Costas Fotakis, IESL-FORTH
Prof. Eleni Papadaki, UoC

http://anatquest.nlm.nih.gov

Methacryloxypropyl trimethoxysilane (MAPTMS),
(2-dimethylamino)ethyl methacrylate (DMAEMA) and methacrylic acid (MAA) as the polymerizable monomers
Zirconium n-propoxide (Zr(OPr)
4
, and the trimethoxysilane groups of MAPTMS serve as the inorganic network forming moieties