Chapter 5: DNA, Gene
Expression, and Biotechnology
Clicker Questions
by Kristen Curran, University of Wisconsin-Whitewater
Which answer will base pair with
the following sequence?
AGTTCTCATGT
1.
2.
3.
4.
AGTTCTCATGT
ACATGAGAACT
TCAAGAGTACA
UCAAGAGUACA
Different people can have free or attached
earlobes. The DNA that encodes for making
free or attached earlobes is called a(n)
________, and there are two different
versions of it, called __________.
1.
2.
3.
4.
allele; genes
trait; alleles
gene; trait
gene; alleles
Which molecule acts as a “middle man”
between the nucleus, where transcription
occurs, and the cytoplasm, where translation
occurs?
1.
2.
3.
4.
DNA
mRNA
Protein
Choices 1 and 3 are
correct.
Classroom Catalyst
Transcription Activity
Which answer shows the correct
orientation of the mRNA sequence
(Phosphate (5’)  Oxygen (3’)) given the
DNA sequence below?
5’-TCATCTATCT-3’
1.
2.
3.
4.
5’-UCAUCUAUCU-3’
5’-AGUAGAUAGA-3’
5’-AGAUAGAUGA-3’
5’-AGATAGATGA-3’
Classroom Catalyst
Translation Activity
Translate the following mRNA:
5’-CGAGCAUGGGCUUUAGG...
1.
2.
3.
4.
MET-GLY-PHE-ARG…
ARG-ALA-TRP-ALA-LEU…
GLY-PHE-ARG-VAL-ARG…
ARG-PHE-GLY-MET-SER…
Which answer shows the mRNA
transcribed from the DNA sequence
below?
5’-TTA TCC TTT ACT CAT-3’
1.
2.
3.
4.
5’-AUG AGU AAA GGA UAA-3’
5’-AAU-AGG-AAA-UGA-GUA-3’
5’- TTA-TCC-TTT-ACT-CAT-3’
5’-UUA-AGG-AAA-TGA-GUA-3’
Which of the following changes (base
substitutions and insertions are underlined
and in bold) would have the most severe
effect on the protein made from the mRNA in
the last question?
5’-AUG AGU AAA GGA UAA-3’
1.
2.
3.
4.
AUG-AGU-AAG-GGA-UAA
AUG-AGU-AAA-GGA-UAG
AUG-GUA-AAG-GAU-AA
AUG-AGU-AAA-GGA-GUA-A
Why can one change in a nucleotide
sequence of a gene cause a
nonfunctional protein to be produced?
1. The change can cause a stop codon to occur early in
translation and the full length protein is not
translated.
2. A substitution can result in one incorrect amino acid
being present in the protein which can affect the
shape of the protein and therefore its function.
3. An insertion or deletion can occur early in the gene
and result in an incorrect translation of the protein.
4. All of the above.
If you were trying to identify the gene for
insulin, which particular step of the
procedure outlined below would you use?
1. Cut human DNA into small pieces with a
restriction enzyme.
2. Amplify the DNA pieces with PCR.
3. Insert the gene into a plasmid and insert
into bacteria.
4. Probe a gene library.
I would want to know if my genome or
that of a loved one carries one or more
copies of a gene that can cause a
genetic disease.
1.
2.
3.
4.
5.
Strongly agree
Agree
Neutral
Disagree
Strongly disagree
Which food below does not
potentially contain GMOs?
1.
2.
3.
4.
5.
Nacho chips
Fruit juice
Corn flakes
Rice
All of the above.
Using the DNA fingerprint information
below, determine which suspect was
present at the crime scene?
1.
2.
3.
4.
Suspect #1
Suspect #2
Suspect #3
All of the above.
Crime
Scene
Suspect
#1
Suspect Suspect
#2
#3
We should pursue cloning in animals
but not humans.
1.
2.
3.
4.
5.
Strongly agree
Agree
Neutral
Disagree
Strongly disagree
We should pursue cloning in humans
for the purpose of developing or
performing medical treatments.
1.
2.
3.
4.
5.
Strongly agree
Agree
Neutral
Disagree
Strongly disagree