Section 10.1 Mendel’s Laws of Heredity pg. 253 WHY MENDEL SUCCEEDED Gregor Mendel (1822-1884) Austrian Monk known as the “father of modern genetics” Found that inheritance follows certain laws later known as Mendel’s Laws of Inheritance Heredity is the passing on of characteristics from parent to offspring Inherited characteristics are called traits (factors) The branch of biology that studies heredity is called genetics – which was founded upon the rediscovery of his work. WHY MENDEL SUCCEEDED Mendel’s pea plant collection contained around 28,000 plants Pea plants reproduce sexually by producing male and female sex cells (gametes) Male gamete forms in pollen Female gamete formed in ovary Fertilization occurs when the male gamete unites with the female gamete Pollination is the transfer of pollen to ovary in a plant – Remove male parts normal reproduction. Snipping the stamen could prevent self-pollination Flower parts A similar figure is in the book on pg. 642 WHY MENDEL SUCCEEDED Cross-pollination is transferring pollen of one plant to the ovary of another plant • Mendel did this to get certain plants to breed with others to be sure of the parents • Mendel was very careful with all of his work Figure 10.1 MENDEL’S MONOHYBRID CROSSES Mendel was able to create tall Figure 10.2 plants and short plants (purebreds) P1 • He referred to the offspring of a purebred tall and a purebred short as a hybrid Short pea plant Tall pea plant • Crossing a 6’ tall plant with a 2’ tall (short) plant resulted in all 6’ tall plants F1 • Crossing the hybrid offspring All tall pea plants resulted in 75% tall and 25% short • P1 refers to the “Parental F2 generation” • F1 (“Filial”) refers to the 3 tall: 1 short offspring MENDEL’S MONOHYBRID CROSSES So what does MONOHYBRID refer to? Referring to figure 10.3: • When Mendel crossed a purebred tall with a purebred short he got all tall plants • When he crossed a purebred purple flower with a purebred white flower he got all purple flowers • He referred to the trait that was observed in these cases as ___________. • The trait that seemed to “disappear” he called _____________. • Mendel concluded that these plants have “factors” that control each of the traits (color, shape, height) • We call these factors genes (parts of DNA) • Alternative forms of genes (tall vs. short or yellow vs. green) are known as alleles. Figure 10.3 Seed color Pod color Pod shape Flower color Flower position round yellow purple axial (side) green inflated terminal (tips) yellow constricted Seed shape Plant height Dominant trait tall Recessive trait wrinkled green white short MENDEL’S MONOHYBRID CROSSES These two alleles for each P1 trait can be expressed as a single letter For plant height we can use the letters “T” & “t” Dominant allele is ___. Tall plant Short plant Recessive is ___. Mendel’s purebred tall F1 plants were “TT” His purebred recessive plants were “tt” Fill in the blanks in the figure to the right which t All tall plants goes where? T TTT t t t MENDEL’S MONOHYBRID CROSSES Mendel concluded that the allele (gene form) of tall plants was dominant to the allele for short plants Confirming that the plants had two alleles for each trait (TT = Tall, Tt = Tall, or tt = short) Knowing that traits are inherited from parents, he also concluded that these alleles are inherited However a plant can only get one allele from each parent The gametes (sex cells) contained either one or the other form of the gene (T or t) The Law of _________________ states that every individual has two alleles of each gene and when gametes are produced, each gamete receives one of these alleles. T PHENOTYPES AND GENOTYPES It’s vocab time… Phenotype refers to the organism’s physical characteristic (what you can see) Ex: Tall Genotype refers to the organism’s genetic makeup (what you can’t see) Ex: TT or Tt Homozygous/purebred represents two alleles that are the same (TT or tt) Heterozygous/hybrid organisms have different alleles (Tt) How are we going to distinguish Homo and Hetero? Law of Dominance states that hybrid organisms (Tt) will express the dominant allele (ex: tall). PUNNETT SQUARES 1905 - Reginald Punnett devised an easy way to find expected genotype proportions of offspring from known parent genotypes based off Mendel’s laws Monohybrid Cross (one trait) Heterozygous tall parent T T T T t T t Heterozygous tall parent t T t t t T TT Tt t Tt tt G= 50% Tt : 25% TT : 25% tt P= 3 Tall : 1 short PUNNETT SQUARES Monohybrid crosses are easy to separate alleles according to Mendel’s Law of Segregation If we have heterozygous parents (Tt X Tt) we can just separate the T from the t For Dihybrid crosses, the gamete separation is a little tricky If we have two parents that are heterozygous for seed shape (Rr) and seed color (Yy) their genotype is RrYy To separate alleles into gametes we use the FOIL method from algebra Round Yellow Round green wrinkled Yellow RrYy makes four different gametes Using the FOIL method we get… RY Ry rY ry wrinkled green MENDEL’S DIHYBRID CROSSES Mendel also crossed plants with two different traits Round=R, wrinkled=r & Yellow=Y, green=y What is the genotype of a purebred (homozygous) plant with Round Yellow seeds? RRYY What is the genotype of a purebred (homozygous) plant with wrinkled green seeds? rryy Purebred (homozygous) Round Yellow seeds X Purebred (homozygous) wrinkled green seeds Result of F1…All plants had Round Yellow seeds However crossing the Dihybrid F1 gives a ratio of 9:3:3:1 Which leads us to Mendel’s second law… The Law of __________________ states that genes for different traits are inherited independently of each other. Round Yellow (RRYY) X wrinkled green (rryy) P1 wrinkled green Round Yellow F1 All Round Yellow F2 9 Round Yellow R_Y_ 3 Round green R_yy 3 wrinkled Yellow rrY_ 1 wrinkled green rryy Gametes from RrYy parent Starting here what are the gametes? rY ry RY Ry RRYY RRYy RrYY RrYy RRYy RRyy RrYy Rryy RrYY RrYy rrYY rrYy RrYy Rryy rrYy rryy RY Ry Gametes from RrYy parent rY ry PROBABILITY Knowing the parents genotype we can predict the probable offspring genotype and phenotype What is the probability of having Rr offspring? 50% What is the probability of having Round offspring? 75% r R RR Rr Rr rr R r PROBABILITY Given the parents genotype and number of offspring, you should be able to predict the number of each genotype and phenotype. PROBABILITY PROBLEM R=Round seeds & r=wrinkled seeds 1. P1 genotype: RR X rr 2. All of the F1 offspring will be ______. 3. Assume 140 F2 offspring are created from F1. 4. ________ will have their parents (F1) genotype. 5. ________ will have Round seeds. 6. ________ will have wrinkled seeds. 7. ________ will have the same genotype as the P1. PROBABILITY PROBLEM (#2) 2. All of the F1 offspring will be ______. P1=RR X rr (always put first parent on top of square) R R Rr Rr Rr Rr r r Rr (Round) 2. All of the F1 offspring will be ___________. PROBABILITY PROBLEM (#4) 4. ________ will have their parents (F1) genotype. F1= All Rr R RR r Rr R Rr rr # of F2 Offspring = _____ 140 Expected % of genotype (Rr) that is same as 50% parents= ______ 70 50% (2/4) of 140 = _____ 140 X .5 = 70 r 70 will have their parents (F1) genotype. 4. ________ PROBABILITY PROBLEM (#5) 5. ________ will have Round seeds. R r R RR Rr r Rr rr # of F2 Offspring = _____ 140 Expected % of Round 75% phenotype = ______ 105 75% (3/4) of 140 = _____ 140 X .75 = 105 105 will have Round seeds. 5. ________ PROBABILITY PROBLEM (#6) 6. ________ will have wrinkled seeds. R r R RR Rr r Rr rr # of F2 Offspring = _____ 140 Expected % of wrinkled 25% phenotype = ______ 35 25% (1/4) of 140 = _____ 140 X .25 = 35 35 will have wrinkled seeds. 6. ________ PROBABILITY PROBLEM (#7) 7. ________ will have the same genotype as the P1 (RR or rr). R r R RR Rr r Rr rr # of F2 Offspring = _____ 140 Expected % of RR or rr 50% genotype = ______ 70 50% (2/4) of 140 = _____ 70 will have the same genotype as the P1. 7. ________ Actual Results of Mendel’s Work