Plant Cell
Mitochondria
organelles where cellular
respiration occurs
Respiration of Germinating Seeds
012-10975 r1.04
Respiration of Germinating Seeds
Introduction
Journals and Snapshots
The Snapshot button is used to capture the
screen.
The Journal is where snapshots are stored
and viewed.
The Share button is used to export
or print your journal to turn in your
work.
Each page of this lab that
contains the symbol
should be inserted into your
journal. After completing a
lab page with the snapshot
symbol, tap
(in the
upper right hand corner) to
insert the page into your
journal.
Note: You may want to take a
snapshot of the first page of
this lab as a cover page for
your journal.
Respiration of Germinating Seeds
Lab Challenge
When plant seeds carry out "cellular respiration" they
produce carbon dioxide.
• Does temperature affect the rate at which seeds
produce carbon dioxide (CO2)?
• Do dormant seeds or geminating seeds produce
more CO2?
Respiration of Germinating Seeds
Background
• All living organisms need energy to survive, grow,
and reproduce.
• Organisms get their energy in the form of ATP
(Adenosine Triphosphate), a small molecule packed
with energy.
• Organisms convert sugars and other
macromolecules into ATP molecules through a
process called cellular respiration.
Respiration of Germinating Seeds
Self-Check
1. What is the main function of ATP in living
organisms?
a) respiration
b) triphosphate
c) motion
d) energy
This image is a reminder to
tap
to take a snapshot
of the page after you have
entered your response.
Respiration of Germinating Seeds
...Background
•
The chemical reaction for cellular respiration is:
C6H12O6 + 6O2 ----> 6CO2 + 6H2O + Energy (ATP molecules)
•
CO2 is a measurable end product of the reaction.
The amount of CO2 created indicates the rate of
cellular respiration occurring inside an organism.
•
The process of cellular respiration occurs in the
mitochondria of all eukaryotic cells.
Respiration of Germinating Seeds
Self-Check
2. Why does this lab involve measuring the amount
CO2 produced by seeds?
a) indicates rate of velocity
b) indicates rate of mortgage interest
c) indicates rate of cellular respiration
d) indicates heart-rate
Respiration of Germinating Seeds
Pre Lab Set Up
(Your teacher may have already completed these steps for you)
• 24 hours prior to the lab:
• Place 20 dry seeds in a beaker and fill the beaker with tap water.
• Put the beaker in a dark location for 24 hours. The seeds will begin to
germinate.
• 30 minutes prior to the lab:
• Place half of the germinating seeds into an ice water bath.
• Allow them to cool before starting the experiment.
Respiration of Germinating Seeds
Safety
• Use all standard laboratory safety procedures.
• DO NOT allow the Carbon Dioxide Gas Sensor to get wet.
Respiration of Germinating Seeds
Materials and Equipment
Collect all of these materials before beginning the lab.
•
•
•
•
•
PASPORT Carbon Dioxide Gas Sensor
PASPORT Extension cable
Sampling bottle (included with sensor)
1 Beaker (1000 mL)
10 dry seeds
CO2 Gas Sensor
• Ice (cubes or crushed)
• Paper towels
• Tap water
• 10 germinating seeds at room
temperature
• 10 germinating seeds in ice water
PASPORT Extension Cable
Respiration of Germinating Seeds
Sequencing Challenge
A. Analyze the data.
B. Measure the CO2
produced by
dormant seeds.
C. Measure the CO2
produced by
germinating seeds.
D. Measure the CO2
produced by cold
seeds.
The steps to the left are part
of the procedure for this lab
activity. They are not in the
right order. Determine the
correct sequence of the
steps, then take a snapshot
of this page.
Respiration of Germinating Seeds
Setup: Dormant Seeds
1. Connect a PASPORT extension cable to
the SPARK Science Learning System.
Plug the CO2 Gas Sensor in the cable
and then press the green button on
the sensor to calibrate it.
2. Place 10 dormant (dry) seeds into the
sampling bottle.
3. Put the end of the CO2 Gas Sensor into
the sampling bottle. Firmly plug the
end of the sampling bottle with the
rubber stopper. Do not let the bottle
tip over!
Q1: Why is it important to
firmly insert the sensor
into the bottle and
create a seal?
Respiration of Germinating Seeds
Q2: What will happen to the
carbon dioxide level inside
the bottle? Draw your
prediction in the graph.*
*To Draw a Prediction:
1. Tap
to open the tool
palette.
2. Tap
then use your
finger to draw your
prediction.
3. Tap
when finished.
4. If you make a mistake, tap
to clear your prediction.
Respiration of Germinating Seeds
Collect Data: Dormant Seeds
Note: Avoid jarring the sample bottle during data
collection, as this can cause erratic results.
1. Tap
to begin collecting data.
2. Record data for 10 minutes, then tap
data collection.
to end
3. Remove the sensor and the dormant (dry) seeds
from the sample bottle.
4. Rinse the sample bottle out with tap water.
Continue to the next page.
Q3: Why do you need to
rinse out the sample
bottle?
Respiration of Germinating Seeds
Q4: What happened to the
amount of CO2 produced
by the dry seeds over
time?
Respiration of Germinating Seeds
Q5: What will happen to the
CO2 level when germinating
seeds are placed in the
bottle?
Draw your prediction in the
graph.*
*To Draw a Prediction:
1. Tap
to open the tool
palette.
2. Tap
then use your
finger to draw your
prediction.
3. Tap
when finished.
4. If you make a mistake, tap
to clear your prediction.
Respiration of Germinating Seeds
Collect Data: Room-Temp Seeds
1. Place ten of the room-temperature germinating seeds into the sample bottle.
2. Re-insert the sensor into the bottle.
3. Tap
to begin collecting data.
4. Record data for 10 minutes, then tap
to end data collection.
5. Remove the sensor and the dormant (dry) seeds from the sample bottle.
6. Rinse the sample bottle out with tap water.
Respiration of Germinating Seeds
Q6: What happened to the
amount of CO2 produced
by the germinating
seeds over time?
Respiration of Germinating Seeds
Q7: How did your predicted
graph line compare with
the actual line?
Respiration of Germinating Seeds
Q8: What will happen to the
CO2 level when
germinating seeds are
placed in the bottle?
Draw your prediction in
the graph.*
*To Draw a Prediction:
1. Tap
to open the tool
palette.
2. Tap
then use your
finger to draw your
prediction.
3. Tap
when finished.
4. If you make a mistake, tap
to clear your prediction.
Respiration of Germinating Seeds
Collect Data: Chilled Seeds
1. Fill the 1000 mL beaker with ice and water.
2. Place ten of the chilled germinating seeds into the sample bottle. Place the
bottle into the icy beaker so the seeds stay cold!
3. Re-insert the sensor into the bottle.
1. Tap
to begin collecting data.
2. Record data for 10 minutes, then tap
to end data collection.
3. Remove the sensor and the chilled seeds from the sample bottle.
4. Rinse the sample bottle out with tap water.
Respiration of Germinating Seeds
Q9: What happened to the
amount of CO2 produced
by the chilled
germinating seeds over
time?
Respiration of Germinating Seeds
Q10: How did your predicted
graph line compare with
the actual line?
Respiration of Germinating Seeds
Data Analysis
1. Determine the change in
CO2 concentration for all
runs, and record the
information in the data
table on the next page.
* To Find the Difference
Between Two Data Points:
1. Tap
to open the tools
palette.
2. Tap
and then tap two
points on the data run.
3. Adjust using both
buttons and then tap
4. Tap
to display the
differences.
Respiration of Germinating Seeds
Data Analysis
2. Record the initial, final,
and change in CO2
concentration for all runs,
in the data table to the
left.
*To Enter Data into a Table:
1. Tap
to open the tool
palette.
2. Tap
then tap a cell in
the data table to highlight
it in yellow.
3. Tap
to open the
Keyboard screen.
Respiration of Germinating Seeds
Analysis
1. How does the rate of CO2 production for dormant seeds compare to the
rate of CO2 production for room-temperature germinating seeds? What
does this information indicate?
Respiration of Germinating Seeds
Analysis
2. How does the rate of CO2 production for room-temperature geminating seeds
compare to the rate of CO2 production for chilled germinating seeds? What
does this information indicate?
Respiration of Germinating Seeds
Analysis
3. How did your predictions compare to the actual data collected? What did
you learn?
Respiration of Germinating Seeds
Synthesis
1. If cellular respiration occurs during seed germination, what would
happen to CO2 levels if you added water to a sample bottle filled with dry
peas?
Respiration of Germinating Seeds
Synthesis
2. What is the chemical equation for cellular respiration? Where does this
process occur in the cell?
Respiration of Germinating Seeds
Synthesis
3. What gaseous molecule is released during cellular respiration?
Respiration of Germinating Seeds
Synthesis
4. Explain why plants need to perform cellular
respiration even though they are photosynthetic.
Respiration of Germinating Seeds
Synthesis
5. What environmental factors can affect the rate of cellular respiration in an
organism?
Respiration of Germinating Seeds
Multiple Choice
1. Cellular respiration takes place in what part of the
cell?
a) chloroplasts
b) nuclei
c) lysosomes
d) mitochondria
Respiration of Germinating Seeds
Multiple Choice
2. In cells, the energy stored in glucose is used to
make what energy rich compound?
a) water
b) ATP
c) ADP
d) DNA
Respiration of Germinating Seeds
Multiple Choice
a) 5-10 minutes
3. According to the graph, the rate of CO2 production
was highest at what time?
b) 180-240 seconds
c) 1200-1800
seconds
d) 25-30 minutes
Respiration of Germinating Seeds
Congratulations!
You have completed the lab.
Please remember to follow your teacher's instructions for cleaning-up and submitting
your lab.
Respiration of Germinating Seeds
References
Images are taken from PASCO documentation, public domain clip art, or Wikimedia Foundation Commons.
http://www.openstockphotography.org/image-licensing/cell-biology/Plant_cell_structure.png
http://commons.wikimedia.org/wiki/File:CDC_limabean.jpg
http://commons.wikimedia.org/wiki/File:ATP-3D-sticks-rotate90.png
http://commons.wikimedia.org/wiki/File:Diagram_of_an_animal_mitochondrion.svg
http://commons.wikimedia.org/wiki/File:Soda_bubbles_macro.jpg
http://commons.wikimedia.org/wiki/File:Pro-Atomo.gif
http://commons.wikimedia.org/wiki/File:Mitochondria,_mammalian_lung_-_TEM.jpg