The study of heredity, transfer of characteristics from parent to offspring. A. The Father of Genetics: Gregor Mendel is known as the Father of Genetics. He studied pea plants, because they were easy to observe and reproduce. Knowing that the male part of the plant produced sperm, and that the female part produced the egg, he was able to join the two reproductive cells. This is called fertilization. B. Inheritance of chromosomes Egg + sperm (meiosis) (fertilization) zygote (earliest stage of development) (mitosis and development) fertilization zygote egg meiosis mitosis & development sperm B. Inheritance of Chromosomes Chromosomes exist in pairs, one from sperm and one from egg. Known as homologous pairs A section of DNA within the chromosome that contains the info to make proteins called a gene. Genes determine our traits. A trait is any physical or physiological characteristics. Ex: eye color, blood type We will all receive the same type of genes but we can get different versions of a gene called an Allele. Ex: Gene: thumb type. Alleles: Hitchhikers thumb or straight thumb. Alleles are represented by letters. A dominant allele is written with a capital. A recessive allele is written with a lowercase. Ex: Hitchhikers thumb (A) or straight thumb (a). *Dominant genes masks/hides recessive. Genotype describes the 2 alleles you received from mom and dad. Homozygous means you received identical copies (purebred) AA – homozygous dominant aa - homozygous recessive Heterozygous means you got one dominant and one recessive (hybrid); Aa. ○ 2 people can have the same appearance but have different genetics: AA vs Aa. Phenotype describes how the alleles are portrayed or look physically. Warm-up Oct. 17th #33 Allele For any trait, such as hair color, there are different forms of the protein that create the different hair colors. Each different form is called an allele. We use capital letters to represent dominant alleles and lower case letters to represent the recessive alleles. Genotype vs. Phenotype Genotype is the genetic makeup (AA, Aa, or aa) Phenotype is the physical trait, a result of the genotype From gene to protein protein transcription translation Warm-up Oct. 18th Mendel’s Principles of Inheritance: Principle of: Dominance Segregation Independent assortment #34 GENOTYPES Homozygous Dominant= BB Heterozygous= Bb Homozygous recessive= bb D. Mendel’s Three Principles 1. Principle of Dominance: dominant alleles can mask/ hide recessive alleles Genotypes: Phenotypes: Homozygous dominant:(AA) Dominant trait is expressed Heterozygous: (Aa) Dominant trait is expressed Homozygous recessive:(aa) Recessive trait is expressed D. Mendel’s Principles 2. Principle of Segregation: during meiosis the alleles separate so each parent can only pass on one allele per trait. We get half of our genes from each parent. D. Mendel’s Principles 3. Principle of Independent Assortment: genes on separate chromosomes are passed on independently (randomly) from one another. E. Genetic Probability Punnett squares are used to predict the outcome of fertilization between the gametes. The sides of the Punnett square are labeled with the genotype from each parent (sperm and egg). Monohybrid Cross- involves only 1 trait. Dihybrid Cross- involves 2 traits. Mendelian Genetics • • What is the phenotypic ratio (compare the result of the genes)? What is the genotypic ratio (compare the genetic combinations)? Father ? ? Ex: Huntington’s disease breaks down the nervous system. It is a dominant trait. If a heterozygous woman marries a man without Huntington's what is the chance their kids will have the disease? _______% Mother H h Genetics Group Practice10 punnett squares Draw a card from the gene stack and one from the parent stack- WRITE THIS DOWN before working the problem Complete the cross by doing a punnett square (alleles are already assigned, see your instruction sheet) Complete the Genotype and phenotype ratios Write what the children will look like: 25% blond hair 75% brown hair Ticket Out the Door Cross a heterozygous man who has dimples with a woman who has no dimples. Set up alleles (letters) Work punnett square Genotype ratio: Phenotype ratio: What are the chances of their child having dimples? Mendel Song http://www.youtube.com/watch?v=mx6m aAOOfxQ October 19th Warm-Up # 35 Trait: Free earlobes- dominant = F Attached earlobes- recessive =f 1. A woman has free earlobes, what are two possible genotypes she might have? 2. The genotype for a man is Ff, what is his phenotype? 3. What is a gene? 5 types Non-Mendelian Inheritance These traits are NOT just dominant or recessive, there for they do not follow the Mendelian genetics. 1. Codominance: Both alleles are equally dominant and will be expressed (in heterozygote). o Phenotype: both versions of the trait show in the heterozygote o Genotype: use 2 different letters. o Ex: Sickle Cell Anemia is a codominant disorder. This means that you will have normal disk shaped cells (N), and sickle shaped cells (S). o 3 Possibilities: Genotype Phenotype NN Normal cells only NS Normal & Sickle cells SS Sickle cells only * Heterozygous genotype is resistant to malaria.* Codominant Example: Cross a black cow with a white cow. Both hair colors are dominant. 2. Incomplete Dominance- neither allele is completely dominant to mask the other. ○ Phenotype: the heterozygote results in a blended phenotype. ○ Genotype: Capital letters for both alleles and use a prime (‘) symbol. ○ Ex: In humans this happens in the genes for curly hair. H= curly, H’= straight. So… Genotype Phenotype HH Curly H H’ Wavy H’ H’ Straight Incomplete Dominance Example Cross a white flowered plant with a pink flower. Both white and red are incompletely dominant. Warm-up Oct. 22nd #36 Codominant If two alleles are codominant, they are equally strong. Both alleles would be seen in the phenotype. An example is crossing a black chicken with a white chicken and the offspring having both black and white feathers. Incompletely Dominant Neither allele is dominant, so when both alleles are present they mix or blend. These alleles are written with ONE letter that has a prime ‘ Non Mendelian Genetics: Codominance: Both alleles are equally dominant and will be expressed Phenotype: 3 possibilities Genotype: 2 Capital letters; BB, WW, or BW Red hair is dominant RR Roan is a mixture of red and white hairRW White hair is dominant WW Incomplete Dominance Phenotype: neither allele is completely dominant to mask the other Genotype: heterozygous RR’ results in a blended phenotype , capital letters for both traits and use a prime ‘ symbol for one RR RR’ R’R’ 3. Multiple Alleles: more than two alleles exist for a trait Blood Types: A, AB, B, O Genotype Phenotype IAIA A or IAi AB IAIB IBIB or IBi (ii)- recessive B O Blood Type Example Cross a heterozygous type A mom with a Type O dad. What is the chance they have a child with Type O blood? 4. Sex-linked Traits: Are you colorblind?? 4. Sex-Linked: trait found on the X sex chromosome. Hemophilia and Color blindness are recessive disorders. Phenotype: Female – less likely but can express a sex-linked trait Male –express sex-linked traits more often because they have only one X sex chromosomes Female Male Genotype Phenotype Genotype Phenotype XA XA Unaffected XA Y Affected XA Xa Carrier No male-carriers for a sex linked disorders. XaXa Affected XaY Unaffected Sex-linked Example Cross a colorblind dad with a mom who is a carrier. What is the chance of this couple having a son who is not colorblind? 5. Polygenetic- Traits that depend on many genes and create a range of phenotypes. NO PUNNETT SQUARE can be used. Instead these traits can be graphed using a bell-shaped curve. There is a wide range of differences. Ex. Height, skin color, weight Warm-up th #37 Oct.25 Sex chromosomes For humans, the last set of chromosomes are the sex chromosomes that determine the gender. Females have two X chromosomes and males have one X and one Y. Examples of sex-linked traits are colorblindness and hemophilia, these traits are carried on the X chromosome. Polygenic trait trait determined by many genes, results in a wide range of phenotypes Examples include height, skin color, hair color Warm-up #38 October 1. th 26 What is a genetic disease? Write down your thoughts 2. From the diseases listed below,. Make three columns on your paper and place each into either the genetic, environmental, or infectious disease column. Cancer heart disease Color-blindness Down’s syndrome Malaria Sickle Cell Anemia Stroke Flu Strep Throat Cystic Fibrosis Hemophilia Klinefelters Type II Diabetes Muscular Dystrophy High Blood pressure Lyme disease HIV Common Cold Warm-up #39 Oct. 30th 1. Achondroplasia is a form of dwarfism inherited as a completely dominant trait. Two achondroplastic dwarfs have a dwarf child; later, they have a second child who is normal. What are the chances of their third child having achondroplasia? 2. Barbara, who is not colorblind, and her husband Bill had a colorblind baby girl. Which statement about this situation is true? FIRST DETERMINE WHAT THE GENOTYPES OF THE PARENTs WOULD BE! a. Barbara’s father must have been colorblind b. Barbara’s mother must have been colorblind c. Bill’s mother must have been colorblind d. Bill must be colorblind Warm-up Oct. 31st #40 Gene Disorders vs. chromosomal disorders Gene disorders: Diseases inherited as a single gene on a single chromosome Arise from mutations on within a single gene Chromosomes disorder: caused by receiving a missing or extra chromosome--- like Down Syndrome Often caused by NONDISJUNCTION during Meiosis Called ANEUPLOIDY Can be detected on a KARYOTYPE: a picture of the chromosomes Peer Assessment Use your rubric to give each group a score for each category (1-4) Add up all the scores for a total BE HONEST!! Warm-up Nov. 1st #41 Autosomal Aneuploidy Having too many or too few chromosomes Caused by mistakes in Meiosis--nondisjunctionhomologous pairs do not separate during meiosis Example: DOWN’s SYNDROME Trait Trait inherited on one of the first 22 pairs of chromosomes… …. NOT sex-linked What environmental and lifestyle factors determine human characteristics? Environmental factors include things like lack of access to clean water, living in an area with air pollution, or living in a densely-populated area with little or no sanitation. Lifestyle factors are the choices people make that impact their health. They include things like smoking, drinking, doing drugs, or eating unhealthy foods. People who have healthy lifestyles and live in unpolluted environments are more likely to be healthy and free of disease than people who have unhealthy lifestyles or who are exposed to lots of pollution. Do genes or the environment influence your traits? Use examples from this activity to defend your view point. EXPLAIN the evidence. H. Genetic Disorders 1. Huntington’s disease- found on chromosome 4, causes brain to break down, loss of muscle coordination, and symptoms often appear in your 40’s. 2. Pattern of Inheritance: Autosomal Dominant Sickle-cell Anemia- leads to misshapen red blood cells, leads to poor circulation and pain. Pattern of Inheritance: Autosomal Codominant (both versions of trait are equally dominant) Heterozygous individuals are resistant to malaria Primarily in African-Americans 3. Cystic fibrosis- increased mucus in lungs and digestive tract,. Pattern of Inheritance: Autosomal Recessive Mostly affects Caucasians. 4. Tay-Sach’s disease- breaks down central nervous system leading to premature death (die before 2). Pattern of Inheritance: Autosomal Recessive Found in Jewish and Pennsylvania Dutch populations. 5. PKU (phenylketonuria)- Inability to break down the amino acid phenylalanine, Can build up in brain and lead to decreased mental function Pattern of Inheritance: Autosomal Recessive Can be controlled by diet 6. Hemophilia- blood does not clot normally Pattern of Inheritance: Recessive sex-linked disorder Mainly affects males. Carriers are females only. 7. Colorblindness Pattern of Inheritance: Recessive sex-linked disorder Mainly affects males. Female carriers only. I. Chromosomal Disorders Inherited due to problems with the ENTIRE chromosome. Caused by a mistake in MEIOSIS called nondisjunction. Nondisjunction is when chromosomes fail to separate properly. This mistake leads to Aneuploidy- an incorrect (extra or missing) number of chromosomes in a fertilized zygote. Non disjunction causes aneuploidy 1. Down’s Syndrome- is caused by an extra chromosome #21 (trisomy 21). Causes mental retardation, heart defects, and an enlarged tongue. 2. Klinefelter’s Syndrome- male has an extra X-chromosome (XXY). Male but develops female secondary sex characteristics, and usually sterile. Use testosterone therapy to treat. 3. Turner’s Syndrome- female with ONLY one X-chromosome (XO). Female with underdeveloped gonads, infertile. J. Nature vs. Nurture: Nature is heredity (your DNA). Nurture is your environment Scientist debate: Which has a greater influence? Example: We use identical twins to study the effects of the environment on genes. Diet: can stop the progression of PKU, and limit the risk for genetic predispositions such as diabetes, heart disease, and certain cancers. Environmental Toxins: environmental factors such as UV radiation or tobacco can directly change our genes (lung/mouth cancer, skin cancer)