The Endomembrane System
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Cellular Membranes
Endomembrane Structure
% total membrane in % total in Pancreatic
Hepatocyte
Exocrine Cell
Endoplasmic Reticulum
(smooth)
16
<1
Endoplasmic Reticulum
(rough)
35
60
Golgi
7
10
Plasma membrane
2
5
Lysosomes
0.4
N.D.
Peroxisomes
0.4
N.D.
Endosomes
0.4
N.D.
Mitochondria
39
21
Nuclear envelope (inner
only)
0.2
0.7
Pulse-Label Autoradiography
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3 minutes
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7 minutes
Pulse-Label Autoradiography
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37 minutes
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117 minutes
The Endoplasmic Reticulum
DiOC6(3)
ER dynamics
Video
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ER / Mitochondria
“Smooth”
ER
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Abnormal Pathology
sER from a chondrocyte
of a patient with
pseudoachondroplasia
RER
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SER
Transition zones
The rER
Supplying the NE
Movin’ on
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Note points of vesicle formation
Vesicular Traffic
donor
membrane
recipient
membrane
vesicle type
cell surface
endosomes
clathrin-coated
rER
Golgi
coatomerII-coated
Golgi
Golgi
coatomerI-coated
Golgi
lysosomes
clathrin-coated
Golgi
cell surface
coatomerI-coated
There are at least several (probably many more) distinct classes
of vesicle coats/sorting mechanisms that function at different
places in the cell
coatomer-,
smoothcoated
vesicles
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Biochemical isolation of
clathrin- coated vesicles
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Clathrin
Animation
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Breakin’ up
is hard to do
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200 nm
100 nm
Dynamin
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shits
wt
Fusion
Just as there are specific mechanisms for sorting and vesicle formation,
there are equally specific mechanisms for vesicle delivery
ts
VSVG ::GFP
transport
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Video
Vesicular stomatitis virus glycoprotein, temp-sensitive folding mutant
The Golgi
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Perinuclear Golgi
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Biochemical stratification
Post-Golgi transport tubules
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Coatamer I Dynamics at the Golgi
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Fluorescence Recovery After Photobleaching (FRAP)
of COPI::GFP
Lysosomes
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Exocytosis/Endocytosis
Peroxisomes
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