Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice

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Review Meeting of the CAS-Croucher Funding Scheme for Joint Laboratories
中科院—裘槎基金联合实验室资助计划项目总结会
Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice
Joint Laboratory for Plant Cell Biology & Rice Genetics
Professor LIN Hong-Xuan
(SIPPE, Chinese Academy of Science)
Professor JIANG Liwen
(The Chinese University of Hong Kong)
Kunming (18th October 2012)
Prof. Lin (Rice Genetics)
• Rice Genetics
• Rice breeding
• Rice Molecular biology
Prof. Lin (Rice Genetics)
Prof. Jiang (Cell Biology)
• Plant Cell Biology
• Protein trafficking and localization
• Organelle biogenesis and function
Prof. Jiang (Plant Cell Biology)
The two PIs
• Genetics vs. Cell Biology
• Excellent track records in respective research areas
• Have on-going collaborative research
• Complement and syngenism
Our goals and research plan
• To understand the mechanisms of yield traits and
stress tolerance in rice
• Potential application
• Important biological questions on rice biology and
biotechnology
• Training of students and researchers
Research Plan
Yield traits of Rice
Mapping and cloning of genes/QTLs
related to yield traits
Functional analysis of
yield trait genes/QTLs
Cell biological analysis of
yield trait genes/QTLs
Molecular mechanisms of yield traits
FAZ1
FAZ1
NIL(GW2)
NIL(GW2)
S-7
S-65
NIL(GL3.1)
S-85
Molecular breeding for
high-yield crops
Research Plan
Stress tolerance traits of Rice
Mapping and cloning of genes/QTLs
related to stress tolerance
Functional analysis of stress
tolerance genes/QTLs
Cell biological analysis of stress
tolerance genes/QTLs
Molecular mechanisms of stress tolerance
Molecular breeding for crops
with improved tolerance
Progress of on-going collaboration
Mapping based cloning and functional analysis of grain length QTL
GL3.1
Cloning of GL3.1
GL3.1
GL3.1 is predicted to
encode a Ser/Thr
phosphatase with kelch
domain
Overexpress GL3.1-WY3 increase the grain length
GL3.1 increase the grain length, weight and yield
The plot grain yield was significantly increased in NIL (+11.1%)
Subcellular location of GL3.1
GL3.1 in both parents was detected throughout the entire cell
Subcellular location of Cyclin-T1;3
When GL3.1 was coexpressed with CyclinT1;3, increased
accumulation of GL3.1
was observed in the
nuclei
• The Croucher Foundation
• The CAS-Croucher Funding Scheme for Joint
Laboratories
• CAS/SIPPE and CUHK
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