Review Meeting of the CAS-Croucher Funding Scheme for Joint Laboratories 中科院—裘槎基金联合实验室资助计划项目总结会 Molecular Mechanisms of Yield & Abiotic Stress Tolerance in Rice Joint Laboratory for Plant Cell Biology & Rice Genetics Professor LIN Hong-Xuan (SIPPE, Chinese Academy of Science) Professor JIANG Liwen (The Chinese University of Hong Kong) Kunming (18th October 2012) Prof. Lin (Rice Genetics) • Rice Genetics • Rice breeding • Rice Molecular biology Prof. Lin (Rice Genetics) Prof. Jiang (Cell Biology) • Plant Cell Biology • Protein trafficking and localization • Organelle biogenesis and function Prof. Jiang (Plant Cell Biology) The two PIs • Genetics vs. Cell Biology • Excellent track records in respective research areas • Have on-going collaborative research • Complement and syngenism Our goals and research plan • To understand the mechanisms of yield traits and stress tolerance in rice • Potential application • Important biological questions on rice biology and biotechnology • Training of students and researchers Research Plan Yield traits of Rice Mapping and cloning of genes/QTLs related to yield traits Functional analysis of yield trait genes/QTLs Cell biological analysis of yield trait genes/QTLs Molecular mechanisms of yield traits FAZ1 FAZ1 NIL(GW2) NIL(GW2) S-7 S-65 NIL(GL3.1) S-85 Molecular breeding for high-yield crops Research Plan Stress tolerance traits of Rice Mapping and cloning of genes/QTLs related to stress tolerance Functional analysis of stress tolerance genes/QTLs Cell biological analysis of stress tolerance genes/QTLs Molecular mechanisms of stress tolerance Molecular breeding for crops with improved tolerance Progress of on-going collaboration Mapping based cloning and functional analysis of grain length QTL GL3.1 Cloning of GL3.1 GL3.1 GL3.1 is predicted to encode a Ser/Thr phosphatase with kelch domain Overexpress GL3.1-WY3 increase the grain length GL3.1 increase the grain length, weight and yield The plot grain yield was significantly increased in NIL (+11.1%) Subcellular location of GL3.1 GL3.1 in both parents was detected throughout the entire cell Subcellular location of Cyclin-T1;3 When GL3.1 was coexpressed with CyclinT1;3, increased accumulation of GL3.1 was observed in the nuclei • The Croucher Foundation • The CAS-Croucher Funding Scheme for Joint Laboratories • CAS/SIPPE and CUHK