Image The orange sections summarize key information and vocabulary terms are underlined. All life depends on energy, or the ability to do work, in order to function. The original source of all energy on Earth is the sun. All cells are constantly needing and using energy. Energy is stored in the chemical bonds of compounds such as carbohydrates [sugars] and lipids [fats] found in living things. http://www.noaanews.noaa.gov/stories2005/images /sun-soho011905-1919z.jpg The ATP created during cellular respiration is one of the most important compounds within cells because it is used to store/release energy. Adenosine Tri Phosphate (AKA ATP) Think of ATP as a fully charged battery, ready to do work for the cell. When the last phosphate bond in the compound are broken in ATP, energy is released. http://www.ustboniface.m b.ca/cusb/abernier/Biologi e/Module1/Images/atp.jpg Observe the simulation; breaking off the last phosphate creates ADP (Adenosine Di Phosphate) and releases energy to be used in the cell. Sketch it! http://student.ccbcmd.edu/biotutorials/en ergy/images/atp.gif two energy equations that relate to energy for living things are photosynthesis in autotrophs, which makes glucose [C6H1206], and cellular respiration, which converts glucose to ATP. http://desertbruchid.net/Scanned_download_f_Fall2010_f/0 5_Photo_CellResp_Relation.GIF The Draw a plant in your notebook. Take a minute to brainstorm anything and everything that you know about photosynthesis, adding arrows to what goes “in” and what comes “out”. Photosynthesis is the process by which autotrophs create glucose using carbon dioxide, water, and energy from the sun. Animation Scene 1 The chemical equation for photosynthesis: 6CO2 + 6H2O + Sunlight a C6H12O6 + 6O2 (Carbon Dioxide) (Water) (Energy) (Glucose) http://www.ap.stmarys.ca/~ishort/Images/Earth/Atmos/pho tosynthesis.jpg (Oxygen) Chloroplasts are the organelles where photosynthesis occurs in eukaryotes, prokaryotes photosynthesize differently – we will review that later. Chlorophyll is the main pigment that absorbs light energy from the sun within the chloroplasts. Animation Scene 2 http://botit.botany.wisc.edu/images/13 0/Ferns/Gametophyte_Images/Chloropl asts_MC.low.jpg Turn and talk to your table mates: How has the artist represented the equation for photosynthesis in this image? Image Sketch a simplified diagram of a chloroplast in your notebooks, include the following labels: Thylakoid Stroma Image Light Dependent Reaction: (Requires water and sunlight) Takes place in a the thylakoid, a sac-like membranes within the chloroplast where chlorophyll absorbs the light energy Light energy transferred to electrons in the electron transport chain Move to the stroma; uses NADP+ and 2 electrons to make NADPH One water molecule is split to produce ATP and release oxygen from the chloroplast Animation Scene 3 Image Light Independent Reaction: Calvin Cycle (Without light) Takes place in the stroma (gel like material outside the thylakoid membranes in the chloroplast) Series of reactions that use CO2 + ATP to make glucose to use for energy Overview Animation http://www.chimicare.org/curiosita/wpcontent/uploads/2011/11/schemasemplificato-fotosintesi-in-un-cloroplasto.gif Cyanobacteria are photosynthetic prokaryotes – but how do they photosynthesize without chloroplasts? oldest known fossils, 3.5+ billion years old contain chlorophyll and have thylakoid-like plasma membranes with multiple layers for photosynthesis http://photosynthesis.sbcs.qmul.ac.uk/ mullineaux/CellBiol.html Cellular respiration occurs in the mitochondria of ALL eukaryotes, while photosynthesis occurs only in plants and other photosynthetic life. It releases energy by breaking down glucose and other food molecules, most often in the presence of oxygen. Animation Scene 1 http://en.wikipedia.org/wiki/File :Auto-and_heterotrophs.png The chemical equation for cellular respiration: C6H12O6 + 6O2 a 6H2O + 6CO2 + ATP (Glucose) (Oxygen) (Water) (Carbon Dioxide) (Energy) http://en.wikipedia.org/wiki/File:Autoand_heterotrophs.png Sketch a simplified diagram of a mitochondrion in your notebooks, include the following labels: Cristae Matrix (L) Image ; (R) Image Glycolysis: Takes place in the cytoplasm Reaction converts glucose (using ATP) in to 2 molecules of pyruvic acid [C3H4O3]. Pyruvic acid then moves through the mitochondrial membrane, converts to acetyl-CoA and gives off CO2. Animation Scene 2 Biology – The Dynamics of Life, pg. 232 Biology – The Dynamics of Life, pg. 232 Citric Acid Cycle [AKA Kreb’s Cycle]: Takes place in the matrix of the mitochondrion Complicated reactions convert acetyl-CoA to ATP and CO2 Uses multiple enzymes and ATP to carry out the process Uses NAD+ and FAD as electron carriers to create NADH and FADH2 Animation Scene 3 Image Electron Transport Chain: Takes place in the folds of the cristae of the mitochondrion Complicated reactions move NADH and FADH2 through transport proteins within the membrane to create ATP and H2O End result = 36 ATP molecules for every glucose Animation Scene 4 http://www.teachersdomain.or g/assets/wgbh/tdc02/tdc02_im g_electronchai/tdc02_img_elect ronchai.jpg Prokaryotes need ATP to fuel cell processes – but how do they create ATP without mitochondria? process occurs in specialized membranes and the cytoplasm http://textbookofbacteriology.net/the microbialworld/Structure.html There are times when mitochondria within your cells cannot get enough O2 to support the cells’ energy needs. Anaerobic respiration is respiration to create ATP in the absence of oxygen, known as fermentation. Fermentation produces many items found in the home Types of Fermentation: Lactic acid fermentation Alcoholic fermentation http://visionhelp.files.wordpress.com/20 12/01/no-symbol.jpeg O2 Lactic Acid Fermentation Fermentation that mainly occurs in the muscle cells of animals and a few other types of cells Lactic acid is produced during this form of respiration; it is filtered out in the liver of animals Causes muscles to be sore after a hard workout Dairy industry uses LAF in bacteria to make cheese Other microbe fermentation creates soy sauce from soy beans End result = 2 ATP molecules for every glucose Alcoholic Fermentation Fermentation that mainly occurs in yeast and a few types of bacteria Ethanol [a type of alcohol] is produced during this form of respiration; it is toxic waste released by the yeast in to their surroundings Wine industry uses AF to make wine; yeasts die in the wine vat when the alcohol concentration reaches 14% End result = 2 ATP molecules for every glucose Video http://academic.pgcc.edu/~kroberts/Lecture/Chapter%205/0523_ProductsOfFerment_L.jpg Fossil record indicates that eukaryotes first appeared ~2 billion+ years ago – how did these cells containing complex organelles, evolve? Endosymbiotic Theory, first proposed by Lynn Margulis of the University of Massachusetts, suggests that chloroplasts and mitochondria [w/ their own DNA & ribosomes] – historically, were prokaryotic organisms that lived within [“living together”] other, larger cells, slowly evolving over time to live as one organism. Video 1 Video 2 Dr. Lynn Petra Margulis, Evolutionary Biologist, 1938-2011 Image http://dontdontoperate.files.wordpress.com/2011/03/endosymb.gif