Congenital cytomegalovirus: a treatable cause of sensorineural hearing loss Dr Simone Walter Consultant Audiovestibular Physician Cytomegalovirus (CMV) Herpes virus, may reactivate Transmitted via body fluids Children and adults: • Flu, cold or asymptomatic Fetus: • congenital infection spectrum: asymptomatic Cytomegalovirus (CMV) symptomatic severe disease 2 Definitions • Congenital CMV (cCMV) CMV infection before birth, diagnosed by testing body fluid samples taken prior to Day 21 …may cause childhood sensorineural hearing loss (SNHL) • Perinatal CMV • Acquired CMV 3 Classification 1. ‘Primary’ or (common) ‘Secondary/recurrent’ (rare) N.B fetus infected in only approx 40% of maternal infections 2. ‘Symptomatic’ or ‘Asymptomatic’ at birth 4 Symptom and Signs at Birth • Intrauterine growth retardation • Microcephaly • Jaundice • Hepatosplenomegaly • Thrombocytopenia • Petechial rash • Seizures • SNHL • Chorioretinitis 5 Burden of Disease • Congenital CMV: 3-5/1000 live births in UK 0.2-2% live births worldwide • Causes 10-20% of childhood SNHL Peckham 1987, Pass 2005, Barbi 2003 • Important cause of neuro-disability (7% cerebral palsy) and vestibular dysfunction 6 How common is hearing loss in cCMV? 2/3 of children with CMV-related SNHL are ‘asymptomatic’ at birth 7 When does the hearing loss occur? • 50% congenital • 50% post newborn hearing screen • most by age 4years Pass 2005 8 cCMV-related SNHL Epidemiology (England) Characteristics of CMV-related SNHL • Progressive >50% • Unilateral 30-50% • Fluctuates 30% • Improvement at one or more frequencies 20-40% • High Frequency 20-30% age 4 months age 15 months age 32 months Dahle 2000, Williamson 1992, Fowler 1997 10 Pathogenesis of CMV-related hearing loss • Cell damage and death • Inflammation • Reactivation • ‘CMV immune escape mechanisms’ • Genetic factors Large CMV infected cells in the Organ of Corti, courtesy of Prof Leslie Michaels Nystad 2007, Schraff 2007 11 Non-specific findings in cCMV • • • • • • • • • • Antenatal illness/fever Premature birth Intrauterine growth retardation Gastro-oesophageal reflux? Prolonged jaundice Microcephaly Hypotonia Delayed motor milestones Developmental delay Cerebral palsy 12 Imaging Cranial ultrasound CT: intracranial calcifications MRI: Static white matter changes Parietal lobe Anterior temporal lobe Polymicrogyra Multifocal lesions and cysts Van der knaap 2004, Manara 2011 13 Investigation for CMV: antibodies Guidelines for cCMV investigation Test maternal antibody (IgG): if negative, exclude cCMV < 1 yr urine/saliva x 2 occasions >1yr IgG antibody If either are positive: request DBS for CMV testing dried blood spot (DBS) (Guthrie card), taken in first week of life Saliva testing • Bottle fed babies-any time • Breast fed babies-1 hour after feed • Leave swab in baby’s mouth for 1 min 16 Testing ‘Guthrie’ cards for CMV DNA 1/2 Dried Blood Spot cut from card Nucleic acid extracted Sensitivity: 71-100% Specificity: 99-100% (Barbi 2006) PCR using primers specific for CMV gene sequences to amplify a specific area of CMV genome. 17 Testing ‘older’ Guthrie cards Guthrie cards received - age of children and proportion testing positive 100 23.2% +ve -ve No. of cards 22.2% 25.7% 26.3% 75 22.7% 50 25 0 <1 year 1-2 2-5 5-10 >10 Age (yrs) 18 Management of cCMV-related SNHL • Infants: refer to Paediatric infectious diseases ASAP • Regular review • Hearing aids • Early referral for cochlear implantation • Check vestibular function and vision • Precautions • Information: CMV Action, NDCS Cochlear implant Kadambari et al 2011 19 CMV and the Vestibular System Pappas 1983 • Hypofunction/failure in 6/11 • Unilateral/bilateral • Independent of SNHL Zagolski 2007 • Vestibular dysfunction more common than SNHL • Gross motor delay 20 Visual complications • • • • • • Symptomatic group mod/severe VI 22% optic atrophy 37% macular scars 13% cortical VI 50% squint 29% • • • • Asymptomatic group mild unilateral VI 1% macular scar squint 1.2% CMV Coats 2000 retinitis 21 Precautions • Handwashing/gloves • Wash toys, eating and drinking utensils, dummies • Avoid kissing mouth-mouth • Condoms • Take care re immuno-compromised e.g. transplant patients • Wait one year before conceiving again Adler et al 2004, Stowell 2014 22 Treatment: Rationale Risk factors for SNHL: • Central nervous system involvement, petechiae, IUGR Williamson 1992, Rivera 2002 • Viraemia Bradford 2005 • Viral load Rivera 2002, Boppana 2005, Walter 2007, Arav-Boger 2007 Boppana 2005 23 Antiviral Therapy • IV ganciclovir or oral valganciclovir • Prevents CMV replication ganciclovir • Side effects: platelets and/or white cells, anaemia, kidney function Valganciclovir: vomiting and diarrhoea Randomised Controlled Trial, Kimberlin 2003: ‘Ganciclovir prevents hearing deterioration at 6 months and may prevent hearing deterioration at > or =1 year’ (CNS affected symptomatic neonates) 24 Duration of therapy Randomised controlled trial CASG 112 All symptomatic neonates: 6 weeks v 6 months valganciclovir Abstract: 6 months of oral VGCV treatment of infants with symptomatic congenital CMV disease improves audiologic and neurodevelopmental outcomes to at least 2 years of age. 25 Can we diagnose CMV-related SNHL in time for treatment? • Integrating CMV screening with newborn hearing screen • CMVBEST study 1: CMV tests offered for ‘referred’ children • Testing kits and questionnaires posted to parents • Outcomes: parental anxiety, comparison of diagnostic tests 26 Newborn Hearing Screening Programme (NHSP) 27 CMV BEST 1 Results Saliva is more feasible than urine Parental anxiety scores not significantly different All cCMV infections detected within 28 days 6/411 referred babies (1.5%) positive for congenital CMV 3/24 (12.5%) infants with SNHL had cCMV All 3 had unilateral SNHL and parents accepted oral valganciclovir Williams et al 2014 28 CMVBEST2: Enhancing Clinical Integration with the Newborn Hearing Screening Programme • Screeners take saliva samples at the point of referral • Aims: 1. Recruit >90% eligible infants 2. Complete cCMV screening, return result to family and GP and commence treatment, as necessary, by 28 days of life 3. Assess feasibility of screeners performing CMV swab (Education package; designed with CMV Action) 29 Future studies: • CMVBEST 3: can targeted screening for cCMV be integrated into the NHSP in 5 regions in England? • CASG 403: ‘Toddler Valgan’ Children age 1 month-4 years with congenital CMV and SNHL 6 weeks valganciclovir v 6 weeks placebo • Vaccines • Antenatal therapy • Avoidance Conclusions CMV causes 10-20% of childhood hearing loss (SNHL) SNHL: any pattern, progresses in 50% Antiviral treatment is available for neonates CMV-related hearing loss is treatable… if diagnosed in time 31 Useful Websites • • • • newborn screening laboratory address: www.newbornscreening.org/laboratories.asp cmvaction.org.uk www.ndcs.org www.ecci.ac.uk 32 Acknowledgements Professor Paul Griffiths Prof Mike Sharland Prof Adrian Davies Dr Sue Luck Dr Seilesh Kadambari Ms Claire Atkinson Dr Eleri Williams Mrs Carmen Burton CMV Action (Proceeds to Royal Free Charity) Thank you for your attention 33 References Peckham CS (1987) Stark O, Dudgeon JA, Martin JA, Hawkins G. Congenital cytomegalovirus infection: a cause of sensorineural hearing loss. Arch Dis Child. Dec;62(12):1233-7 Pass RF (2005) Congenital Cytomegalovirus Infection and Hearing Loss. Herpes Oct; 12(2): 50-5 Williamson WD (1992) Demmler GJ, Percy AK, Catlin FI. Progressive hearing loss in infants with asymptomatic congenital cytomegalovirus infection. Pediatrics. Dec;90(6):862-6 Dahle AJ (2000) Fowler, KB Wright JD et al Longitudinal investigation of hearing disoders in children wih congenital cytomegalovirus. J Am Acad Audiol; 11:283-290 Rivera LB (2002) Boppana SB, Fowler KB, Britt WJ, Stagno S, Pass RF. Predictors of hearing loss in children with symptomatic congenital cytomegalovirus infection. Pediatrics. Oct;110(4):762-7 Zagólski O (2007) Vestibular-evoked myogenic potentials and caloric stimulation in infants with congenital cytomegalovirus infection.J Laryngol Otol. Sep 21;:1-6 Kadambari S (2011), Luck, S, Griffiths, P, Sharland, M, Evidence based management guidelines for the detection and treatment of congenital CMV, Early Human Development 87 (2011) 723–728 34 References Hicks T (1993) Fowler K, Richardson M, Dahle A, Adams L, Pass R Congenital cytomegalovirus infection and neonatal auditory screening. J Pediatr. Nov;123(5):77982 Fowler KB (1997) McCollister FP, Dahle AJ, Boppana S, Britt WJ, Pass RF. Progressive and fluctuating sensorineural hearing loss in children with asymptomatic congenital cytomegalovirus J Pediatr. Apr;130(4):624-30 Fowler KB (1999), Dahle AJ, Boppana SB, Pass RF J Newborn hearing screening: will children with hearing loss caused by congenital cytomegalovirus infection be missed? Pediatr. Jul;135(1):60-4 Lee DJ, Lustig L, Sampson M, Chinnici J, Niparko JK (2005) Effects of cytomegalovirus (CMV) related deafness on pediatric cochlear implant outcomes Otolaryngol Head Neck Surg. Dec;133(6):900-5. Kimberlin DW et al (2008) Pharmacokinetic and Pharmacodynamic Assessment of Oral Valganciclovir in the Treatment of Symptomatic Congenital Cytomegalovirus Disease.J Infect Dis. Feb 15 Eleri J Williams, Kadambari S, Berrington JE, Luck S, Atkinson C, Walter S, Embleton ND, James P, Griffiths P, Davis A, Sharland M, Clark JE. Feasibility and acceptability of targeted screening for congenital CMV-related hearing loss, Arch Dis Child Fetal Neonatal Ed 2014;0:F1–F7 35 References Kimberlin DW et al (2003) Effect of ganciclovir therapy on hearing in symptomatic congenital cytomegalovirus disease involving the central nervous system: a randomized, controlled trial J Pediatr. Jul;143(1):16-25. Boppana SB (2005) Fowler, KB, Pass RF, Rivera LB, Bradford RD, Lakeman FD, Britt WJ Congenital cytomegalovirus infection: association between virus burden in infancy and hearing loss. J Pediatr. Jun;146(6):817-23 Shibata M (1994) Takano H, Hironaka T, Hirai K. Detection of human cytomegalovirus DNA in dried newborn blood filter paper. J Virol Methods. Feb;46(2):279-85 Barbi M (2006) Binda S, Caroppo Diagnosis of congenital CMV infection via dried blood spot Rev Med Virol 16;6:385-92 Barbi M (2003) Binda S, Caroppo S, Ambrosetti U, Corbetta C, Sergi P A wider role for congenital cytomegalovirus infection in sensorineural hearing loss. Pediatr Infect Dis J. Jan;22(1):39-42 Smets K et al (2006)Selecting neonates with congenital CMV infection for ganciclovir therapy Eur J Pediatr Dec;165(12):885-90 36