Non-TransfusionDependent Thalassemia Ashutosh Lal, MD Northern California Thalassemia Center UCSF Benioff Children’s Hospital Oakland Thalassemia Syndromes: Many diagnoses ATRX 0.5% ß Thal Major 38% Hb H 19% Hb H/CS 8% A Thal Major 0.5% Cß0 0.5% • Oakland Data (n=203) ß Thal Int 11% Eß+ 6% Eß0 14% A Trip/ß Thal Trait 2% What is the proportion of non-transfusion-dependent thalassemia Non-transfused 27% Intermittentlytransfused 17% Transfusiondependent 56% • Not a true representation of NTDT • The real number of non-transfused patients is likely 5-10 times n=203, Oakland data Age profile of patient population in Oakland • Many non-transfusion-dependent patients stop regular follow up 30 50 Transfusion-Dependent 20 10 Female Male 40 Number of patients Number of patients Female Male Non-Transfusion-Dependent 30 20 10 0 0 5 15 25 35 45 Age (years) 55 65 5 15 25 35 45 55 Age (years) Oakland data Thalassemia Syndromes: a continuum No Transfusions Trait Occasional Transfusions Intermedia Regular Transfusions for Symptoms Regular Transfusions for Survival Major Causes of Non-Transfusion Dependent Thalassemia Beta Thalassemia Intermedia • • • • • Beta 0 and mild beta mutation Two mild beta mutations Two beta mutations plus high fetal hemoglobin One beta mutation plus increased alpha genes Single unstable beta mutation E-Beta Thalassemia • Hb E mutation with beta mutation • E and beta mutations with alpha deletion • E and beta mutations with high fetal hemoglobin Alpha Thalassemia • Deletion of three alpha genes • Deletion of 2 alpha genes plus mutation in one alpha gene Transfusion Requirement N u m b e r o f p a tie n ts N ever 80 I n te r m itt e n t R e g u la r 60 40 20 0 T h a l H e te ro E 0 E + In te r m e d ia T h a l T h a l T h a l H bH H b H /C S Oakland data Beta-thalassemia intermedia: Evolving Management Thal Int, 42 years old Thal Int, 16 years old • • • • • • • • • • • • Diagnosed at 1 year Hemoglobin 6-7 g/dL No blood transfusions At 10 years: heart failure Splenectomized Hemoglobin increased to 8 Intermittent transfusions At 30 years: Pulmonary hypertension, right heart failure • Started on regular transfusions Diagnosed at 2 years Hemoglobin 6-7 g/dL No blood transfusions Good energy level, participates in sports, swimming and soccer • At 14 years: Fatigue, splenomegaly, growth delay • No response to hydroxyurea • Started on regular transfusions E-Beta0 Thalassemia • 11 years old, diagnosed with E beta thalassemia at 2 years • Well during infancy • Sick as toddler frequent ER visits for fever; pneumonia • School: Tired compared with peers, needs frequent rest, chooses less active play • 3 transfusions in one winter: fall in hemoglobin during infections • Baseline hemoglobin from 5.8 to 6.5 g/dL • No response to HU • Started on regular transfusions at 6 years E-Beta0 Thalassemia plus alpha0 trait • • • Younger brother 8 years old Hemoglobin level 9.8 g/dl Asymptomatic Heterozygous Beta thalassemia intermedia • Now 36 years old: Dx at 8 months: a little pale, fatigued, poor appetite • Baseline hemoglobin level 7.5 to 9 g/dL • First transfusion at 18 years for aplastic crisis. • Cholecystectomy at 22 years, transfusion prior to surgery • Pregnancy at 34 years, hemoglobin dropped from 7 to 4 g/dL, transfused intermittently during pregnancy • • • • Liver 4 cm, Spleen 8 cm Hemoglobin 6.9 g/dL Ferritin 1830; Liver iron concentration 31.2 mg/g dry-wt Electrophoresis: Hb A2: 4.8%, Hb F 1.2% β Globin gene: • Heterozygous IVSI-1, G->A (β0/βA) α Globin gene: • Heterozygous alpha anti-3.7 triplication (ααα/αα) HbH Constant Spring Age in years Transfusion Events Comparison with Thalassemia Major More in thalassemia major More in non-transfusion dependent thalassemia • Iron overload • Early need for effective chelation • Consequences of iron overload • Anemia • • • • • • Endocrinopathies Hypogonadism Osteoporosis Heart disease Liver disease Transfusion-transmitted infections • Hospital visits • Sudden fall in hemoglobin • Extramedullary masses • Splenomegaly • Pulmonary hypertension • Thrombosis • Leg ulcers • Silent Cerebral Infarction Clinical Management Guidelines Oakland Standards of Care The initial clinic visit Review laboratory results • Hematological • Electrophoresis • DNA tests Counseling • Discuss probable outcome and uncertainties • Stress that close follow up is essential to make informed decisions Introduce the care team • Physician, Nurse Practitioner, Social Worker, Clinic Coordinator, Dietician, Genetic Counselor • Provide support Montioring • Frequency of visits • Initially every month, then 2 months, then 3-12 months • Growth • Height and weight, pubertal development • Nutrition • Folate, vitamin D, avoiding supplemental iron • Counseling for risk during infections • Building relationship • Accessibility, social work assessment, diagnosis card Management of Fever Two major risks during fever • Worsening of anemia • Serious sepsis Patients with fever >100.4 F seen on the same day • Exception – Deletional hemoglobin H disease – can be seen next day During the clinic or ER visits • Check hemoglobin, reticulocyte count and bilirubin • Admit for observation or transfusion if the hemoglobin low • Antibiotic treatment may be needed Splenectomized patients with a fever • Should be seen on the same day • Given a dose of intravenous antibiotic, admission recommended Options for treatment Observation and supportive care • Folate, nutrition, regular monitoring Hydroxyurea • Other experimental agents to increase fetal hemoglobin Splenectomy Regular transfusions Alternatives to long-term transfusions • Bone marrow transplantation • Gene Therapy Splenectomy • Splenectomy is NOT recommended as a means to delay or prevent the need for regular transfusions • Hb H Constant Spring is an exception Number of Patients 30 Splenectomized Non-Splenectomized 25 20 15 10 5 0 Never Occasional Regular E Beta Thalassemia Oakland data Hydroxyurea • Benefit of Hydroxyurea is uneven • Certain mutations predict better response to hydroxyurea • XmnI polymorphism • Lepore or δβ-thalassemia • Patients with extramedullary pseudotumors • Hydroxyurea starting dose of 10 mg/kg/day, not exceeding 20 mg/kg/day • Response evaluated after 3 and 6 months of therapy • Hemoglobin level increase of >1 g/dl at 6 months • Discontinue in patients not showing response Specific Management: Assessing the need for transfusions Growth problems Fatigue Quality of life Splenomegaly Extramedullary hematopoiesis Pulmonary Hypertension Pain Intermittent transfusions • Recommended when hemoglobin falls <6 g/dL • Frequent episodes mean regular transfusions needed Transfusion therapy: When to transfuse Beta thalassemia • Patients with hemoglobin consistently <7 g/dL should start regular transfusions E Beta Thalassemia • Many patients do well with hemoglobin 6-7 g/dL • Consider growth, fatigue, splenomegaly Hemoglobin H Disease • Transfusions are not necessary for management Hemoglobin H Constant Spring • Intermittent transfusions are generally needed • Regular transfusions are usually not recommended Iron Overload • Development of iron overload is inevitable, irrespective of transfusion status • • • • Extra iron is absorbed from food Iron deposition is cumulative and age-dependent Serum ferritin under-estimates the liver iron Cardiac iron deposition less common L iv e r Ir o n C o n c e n tr a tio n • Liver damage • Hormone deficiencies 40 m g /g 30 20 10 U p p e r L im it 0 Oakland data: NTDT patients Thal Thal Thal Thal N o Tx In t T x N o Tx In t T x Assessment of Iron Overload • Measure serum ferritin • Measure liver iron concentration when ferritin >300 ng/mL • Measure cardiac iron if LIC >15 mg/g • Evaluate for hormone deficiencies Treatment of Iron Overload • Non-transfused patients >10 years with ferritin >300 ng/mL and LIC >5 mg/g • Earlier for intermittently transfused patients • Deferasirox is the preferred chelators • Dose is 50% of that used for thalassemia major • Goal: reduce ferritin <200 ng/mL, LIC <5 • Stop therapy, resume monitoring Fertility • Fertility is usually not affected • Genetic Counseling: Partner testing is essential Pregnancy • Pregnancy: Consider transfusions during pregnancy when hemoglobin <8 g/dL Quality of Life • Monitored for deterioration in QOL with age • Chronic fatigue, difficulty in coping at work • Family stress • Chronic pain • Psychosocial assessment, support and counseling Barriers to Care • Lack of regular follow up • Lack of evaluation at Comprehensive Thalassemia Center • Lack of medical insurance Elliott Vichinsky, MD Medical Director of Hem/Onc Ashutosh Lal, MD Director of Thalassemia Program Lynne Neumayr, MD Administrative Director Sylvia Titi Singer, MD Associate Hematologist Carolyn Hoppe, MD Director, Hemoglobin Ref Lab Drucilla Haines, PNP Clinical Nurse Practitioner Wendy Murphy, MSW Thalassemia Social Work Laurice Levine, MA, CCLS Thalassemia Outreach Shanda Robertson Database Manager Ellen Fung, PhD Nutrition Scientist Marcela Weyhmiller, PhD Iron Program