Postgraduate Quiz Instructions • All slides carry equal marks • There is no negative marking • Each slide will be given 30 seconds to 1 minute after reading out the question All the Best! Q1. • 18 years female presented with high grade fever • She was a known case of HIV PBF • Identify the abnormality • Advice Cytochemistry Q2. • 48 years ♀ presented with weakness & variable cytopenias • She was being treated for depression Trephine biopsy • Identify the abnormality • Advice Confirmatory test Q3. • 15 years male presented with fever with chills PBF • Identify the abnormality • Name the Vector Q4. HPLC graph from BioRad Classic • Identify the abnormality • What will be the expected clinical phenotype? Q5. • Identify the preparation • Enumerate 2 conditions where the test may be positive Q6. 64 year male with splenomegaly. • Interpret the flow cytometry • Suggest a molecular confirmatory test for this disease? Q8. Describe the morphology of the cell in the peripheral blood film. Mention 2 conditions where this can be seen Q9. Normal Patient Spot the abnormality A B Name 2 conditions where the abnormality can be seen Methemoglobin levels: 7.5% & after blood transfusion- 0.3% ? Father (Case II) Son (Case I ) OxyHb is capable of reversible oxygenation; its heme iron is in the reduced (or ferrous) state MetHb: Heme oxidizes spontaneously & is incapable of reversible oxygenation Q10. • 21 years ♀ presented with gum bleeds & skin nodule • Blood counts showed increased TLC with thrombocytopenia BM aspirate 1. Suggest possible diagnosis 2. Suggest associated cytogenetic abnormality • 2 year male presented with epistaxis • Coagulogram: PT- 13 sec, APTT-27 secs, N platelets Platelet aggregation Physiological Agonists ADP 10mM Collagen 2mg/ml Epinephrine 10mM Arachidonic acid 0.5mM • What is the diagnosis? • What will you find in a finger prick smear • What is the mode of inheritance of the condition? Q6. Q 10. • Name any 2 agents that can be used for decalcification of bone marrow biopsies. Q 11. Kidney biopsy from a 33 yr female with seizures and acute renal failure following fever for a day. • What is the diagnosis? • What will the PT & PTTK results? • Mention one therapeutic modality. Two siblings ages 3 & 5 years presented with pallor and mild hemolytic facies BM was done to investigate cause of anemia Q 12. BM aspirate Descibe the BM findings Any confirmatory tests? Q 13. 6 months child presented with progressive anemia and splenomegaly Screening for thalassemia was negative Trephine biopsy Descibe the findings Any special stains? Q 14. • 13 yrs male presented with fever & echymosis for 3 days • Blood cell counts showed anemia, leucocytosis and thrombocytopenia Describe the BM findings and interpret the MPO stain BM MPO Q 15. Describe the BM aspirate finding