Magnus Pro Training Presentation
Image analysis Software
Note: The contents of this presentation are only showing the procedure for
calibration, counting & generating reports. The dummy data have been shown just
for training purpose
Calibration
• For any measurements calibration is required to be
•
•
•
performed through standard stage micrometer.
Formulae : Micrometer scale 1mm = 1000 Micron
(e.g. 0.01mm= 10 micron)
It should be performed on all objectives of microscope
where digital camera or MIPS are installed.
In case of readjustments or new replacement of any part
of microscope or camera, the calibration should be done
again.
(contd.)
Select Magnification before
calibration
Before going to measurement,
one has to select appropriate
magnification of calibration
Calibration (contd.)
procedure
• Focus stage micrometer under the first objective
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•
•
•
of microscope in X direction.
Capture image with proper names.
Focus stage micrometer under the first objective
of microscope in Y direction.
Capture image with proper names.
Repeat above procedure for any other objectives
like 10X, 40X, 100X etc. (as per availability)
(contd.)
Screen Dumps (calibration)
For objective 100X in X and Y
direction
Give the
suitable name
of calibration
and save
Load
image
captured
in X
axis for
100X
obj.
Move line
slowly with
the help of
Zoom
Preview and
release the
line once it
reaches to the
other line
If line is for
e.g.. 60
pixels
Then write
down same
reading in the
Scale X
value
Zoom
Preview
Calibration Procedure for X direction
Give the
suitable name
of calibration
and save
Load
image
captured
in Y
axis for
100X
obj.
Move line
slowly with
the help of
Zoom
Preview and
release the
line once it
reaches to the
other line
Zoom
Preview
If line is for
e.g.. 60
pixels
Then write
down same
reading in the
Scale Y value
Calibration Procedure for Y direction
Measurement
• The measurements can be obtained after
pixel calibration.
• The units of measurements depends on
the currently selected spatial calibration
(for e.g. micron or inches or millimeter
etc.)
Measurements of
selected images
Images on
which
measurements
are calculated.
Selected calibration
Count
• Used to automatically identify objects in
an image.
• To get the several feature of the
measurements.
Automatically
identified objects
in an image
Click Count to
count the no. of
objects
obtained the
several
features of the
measurements
Automatic Report Generation
Select File for
report to be
generated
Click Report data
and select
appropriate data on
which report is to
be generated